The present study aimed to evaluate the antibacterial potency of grinded crude material (root of Glycyrrhiza glabra) against some gram-positive and gram-negative bacterial strains. Two solvents (methanol and acetone) were used to extract the phytochemicals from the test material. Four different concentrations (100%, 75%, 50% and 25%) of methanolic and acetonic extract were used to investigate the inhibiting properties against Salmonella typhi, Escherichia.coli, Vibrio cholerae, Staphylococcus aureus, Bacillus cereus and Bacillus subtilis strains. Among methanol and acetone extracts, later exhibited low antibacterial activity. The 100% (w/v) concentration of both extracts showed maximum inhibition against B. subtilis followed by E. coli, S. aureus, B. cereus, S. typhi and V. Cholerae. Maximum activity in acetonic extract was obtained against B. cereus followed by S. typhi, E. coli, V. cholerae and S .aureus and minimum in B. subtilis. A reverse pattern of inhibition activity was found in both extacts (methanolic and acetonic) against B. subtilis. Maximum activity was found in methanolic extract against B. subtilis (18.6 mm) but it was only 14.3 mm against this strain in acetonic extract. The antibacterial activity of the crude samples corresponded to that of concentration. Hence there was positive correlation of antibacterial activity with the test material.
Antibacterial actvity, Glycyrhiza glabra, Gram-positive bacteria, Gram-negative bacteria
Biondi, D.M., Rocco, C and Ruberto, G. (2003). New Dihydrostillbene derivatives from leaves of Glycyrrhiza glabra and evaluation of their antioxidant activity. Journal of Natural Products, 66:477-480.
Freedman, Samuel O., Shulman, Robert., Krupey, John and Sehon, A.H. (1964). Antigenic properties of chlorogenic acid. Journal of allergy, 35(2):97
Fukai, T., Tantai, L and Nomura, T. (1996). Isoprenoid substitute flavonoid from Glycyrrhiza glabra. Phytochemistry, 43:531-532.
Fukai,T., Ali, M., Kaitou, K., Kanda, T., Terada, S and Nomura, T. (2002a). Anti-Helicobacter pylori flavonoid from licorice extract. Life Sciences, 71:1449-1463.
Fukai, T., Marumo, A., Kaitou, K., Kanda, T., Terada, S and Nomura T. (2002b). Antimicrobial activity of licorice flavonoids against methicillin-resistant Staphylococcus aureus. Fitoterapia, 73: 536-539.
Fukai, T., Satoh, K., Nomura, T., Sakagami, H. (2003). Preliminary evaluations of antinephritis and radical scavenging activities of glabridin from G. glabra. Fitoterapia, 74: 624-629.
Gatto, Maria Teresa., Serena Falcocchio., Eleonora Grippa., Gabriela Mazzanti., Lucia Battinelli., Giovanni Nicolosi., Daniela Lambusta and Luciano saso. (2002).Antimicrobial and Antilipase activity of quercitin and its C2-C16 3-OAcyl-Esters. Journal of bioorganic and medicinal chemistry, 10:269-272.
Glenn, Asaeda., Caicedow, Gilbert and Swanson, Christopher (2005). Fried Rice Syndrome. Journal of Emergency Medical Services, 30 (12): 30â€“32
Gupta, V.K., Fatima, A., Faridi, U., Negi, A.S., Shanker, K., Kumar, J.K., Rahuja, N., Luqman, S., Sisodia, B.S., Saikia, D., Darokar, M.P. and Khanuja, S.P.S. (2008). Antimicrobial potency of Glycyrrhizaglabra roots. Journal of Ethnopharmacology, 116: 377-380.
Kaul, T.N., Middleton Jr, E and Ogra, P.L. (1985). Antiviral effect of flavonoids on human viruses. J. Med. Virol, l 15, 71-79.
Kinoshita, T., Saitoh, T and Shibata, S. (1976). Flavonols of licorice root. Chem Pharma Bull, 24: 991-994.
Laxmi, Ahirwal., Singh, Siddhartha and Mehta, Archana (2011). Antimicrobial Screening of Methanol and Aqueous extracts of SwertiaChirata. Int J PharmaSci, 3: 142-146.
Mahmood, N., Piacente, S., Pizza, C., Burke, A., Khan, A.I and Hay, A.J. (1996). Biochem. Biophys. Res. Commun. 229 :73
Makky, E.A., Yusoff Mashitah, M and Ibrahim M.M. (2012). Impact of medicinal plants phytocomponents against antibiotic resistant bacteria. Journal of Chemical and Pharmaceutical Research, 4(1): 881-893.
Meena, Ajay Kumar., Singh, Arjun., Sharma, Kiran., Kumari, Suman and Rao M.M. (2010). Physicochemical and preliminary phytochemical studies on the rhizome of Glycyrrhiza glabra. Int. J. of Pharmacy and Pharmaceutical Science, 02:48-50.
Mitscher, L.A., Park, S., Omota, S., Clark, G.W. and Clark, D. (1978). Antimicrobial agents from higher plants, Glycyrrhiza glabra L. I. some antimicrobial isoflavans, isoflavenes, flavanones and isoflavones. Heterocycles, 9: 1533-1537.
Mitscher, L.A., Park, Y.H., Clark, D and Beal, J.L. (1980). Antimicrobial agents from higher plants, Antimicrobial, isoflavanoids and related substances from Glycyrrhiza glabra L. var. typical. Journal of Natural Products, 43: 259-269.
Mou-Tuan Huang., Robert C. Smart. and Ching-Quo Wong. (1988). Inhibitory Effect of curcumin, chlorogenic acid, caffeic acid and ferulic acid on tumor promotion in mouse skin by 12-o-Tetradecanoylphorbol-13-acetate. American Aassociation for Cancer Research, 48: 5941-5946.
Nakane, H. and Ono, K. (1990). Mechanism of inhibition of various cellular DNA and RNA polymerases by several flavonoids. J. Biochem.,108(4):609-13.
Rigano, D., Formisano, C., Basile, A., Lavitol, A., Senatore, F., Rossellis, S and Bruno, M. (2007). Antibacterial activity of flavonoids and phenyl propanoids from Marrubium globosum ssp. Libanoticum. Phytother Res,21(4): 395-7.
Saitoh, T., Noguchi, H and Shibata, S. (1978). A new isoflavone and the corresponding isoflavanone of licorice root. Chem Pharma Bull, 26: 144-147.
Tang, W and Eisenbrand G. (1992). Chinese drugs of Plant Origin. Springer-Verlag, Berlin, Germany; pp: 567-588.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)