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Gokil Prasad Gangwar

Abstract

In the present investigation, fungal and bacterial bioagents which were effective against pathogen of bacterial leaf blight disease of rice (Xanthomonas oryzae pv. oryzae) was tested for their effect on germination of rice seeds and growth of nursery seedlings under laboratory and glasshouse conditions. Two isolates of fluorescent pseudomonas isolated from rice phylloplane (Pf 83 and FLP 88), Trichoderma species were isolated from soil of rice field (isolate 40) and Trichoderma harzianum isolated from rice phylloplane. Two commercial formulations (PBA-1 and PBA-2) were tested in the present investigation and compared with chemical treatment (0.76 g Emison + 0.18 g Streptocycline / kg seeds) and untreated check. Under laboratory conditions, chemical treatment was found most effective in increasing seed germination (43.90%) followed by Pf 83 and PBA-1 (34.15 %). Bioagent formulations were significantly effective in increase in root and shoot length as compared to check and chemical treatment. Under glasshouse conditions, maximum increase (29.42%) in seedlings emergence was exhibited by chemical treatment. Maximum increase in root length (100.15%) and shoot length (50.06%) was recorded with FLP 88 and T. harzianum, respectively. Bioagent formulations also increased fresh and dry root weight over check and chemical treatment. All bioagents formulations were effective over chemical treatment and check in increasing fresh shoot weight. Results of the present study revealed that in the absence of pathogen of bacterial leaf blight of rice, fungal and bacterial bioagents significantly enhanced germination of rice seeds and growth of nursery seedlings.

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Keywords

Bacterial leaf blight of rice, Growth promotion, Pseudomonas spp., Rice, Trichoderma harzianum

References
Bailey, B.A. and Lumsden, R.D. (1998). Direct effect of Trichoderma and Gliocladium on plant growth and resistance to pathogens. In : G.E. Harman and C.P. Kubicek (eds.), Trichoderma and Gliocladium Vol. 2. Taylor and Francis, London, pp. 185-204.
Baker, R. (1986). Biological control: an overview. Can. J. Pl. Path., 8: 218-221.
Baker, R. and Scher, F.M. (1987). Enhancing the activity of biological control agents. In Chet, I. (ed.), Innovative Approaches to Plant Disease Control. Wiley Intersci. New York, U.S.A. pp. 1-18.
Chao, W.L., Nelson, F.B., Harman, G.E., and Hoch, H.C. (1986). Colonization of the rhizosphere by biological control agents applied to seeds. Phytopathol., 76: 60-65.
Dubey, S.C. (1995). Evaluation of fungal antagonistic against Thanatephorus cucumeris causing banded blight of rice. Paper presented at the first International symposium on Rhizoctonia, ISR’95 at Leeuwenhorst Congress Centrum Noordwijkerhout, Netherland. pp. 27-30.
Gangwar, G.P. (2012). Efficacy of commercial formulations of bioagent against bacterial leaf blight of rice. Ann. Pl. Protec. Sci., 20(2): 389-391.
Gangwar, G.P. (2013). Efficacy of different isolates of fluorescent pseudomonads against bacterial leaf blight of rice. Afr. J. Agric. Res., 8(37): 4588-4591.
Gangwar, G.P. and Sinha, A.P. (2010a). Comparative antagonistic potential of Trichoderma spp. against Xanthomonas oryzae pv. oryzae. Ann. Pl. Protec. Sci., 18: 458-463.
Gangwar, G.P. and Sinha, A.P. (2010b). Evaluation of fluorescent pseudomonads against Xanthomonas oryzae pv. oryzae causing bacterial leaf blight of rice. Ann. Pl. Protec., Sci. 18: 532-534.
Gangwar, G.P. and Sinha, A.P. (2012a). Comparative antagonistic potential of fungal and bacterial bioagents against isolates of Xanthomonas oryzae pv. oryzae. Ann. Pl. Protec. Sci., 20: 154-159.
Gangwar, G.P. and Sinha, A.P. (2012b). Evaluation of Trichoderma spp. and fluorescent pseudomonads for the management of bacterial leaf blight of rice. Indian Phytopath., 65(1): 89-91.
Harman, G.E. (2000). Myths and dogmas of Biocontrol: Changes in the perceptions derived from research on Trichoderma harzianum T22. Plant Dis., 84: 377-393.
Harman, G.E., Chet, I. and Baker, R. (1980). Trichoderma hamatum effects on seed and seedling disease induced in radish and pea by Pythium spp. and Rhizoctonia solani. Phytopathol., 70: 1167-1172.
Imam, M.K., Badawy, F.H. and El-Hudairy, A. (1977). Response of tomato and two other vegetable crops to inoculation with Azotobacter. The Libyan J. Agric., 6: 156-167.
Kempe, J. and Sequeira, L. (1983). Biological control of bacterial wilt of potatoes, attempts to induce resistance by treating tubers with bacteria. Plant Dis., 67: 499-503.
Kloepper, J.W., Schroth, M.N. and Miller, T.D. (1980). Effects of rhizosphere colonization by plant growth promoting rhizobacteria on potato plant development and yield. Phytopathol., 70: 1078-1082.
Mishra, D.S. and Sinha, A.P. (1998). Plant growth promotion by some biocontrol agents. In: Fiftieth Annual Meeting of Indian Phytopathological Society and National Symposium on “Present Scenario in Diseases of Oilseeds and Pulses” held at Aurangabad, Maharastra, India. (Abstr.).
Mishra, D.S. and Sinha, A.P. (2000). Plant growth-promoting activity of some fungal and bacterial agents on rice seed germination and seedling growth. Trop. Agric., 77: 188-191.
Mukherjee, P.K. and Mukhopadhyay, A.N. (1995). In situ mycoparasitism of Gliocladium virens on Rhizoctonia solani. Indian Phytopath., 48: 101-102.
Mukhopadhyay, A.N. (1996). Recent innovations in plant disease control by ecofriendly biopesticides. In Presidential address, Agriculture Science section, 83th Indian Science Congress, Patiala, India.
Mukhopadhyay, A.N., Shrestha, S.M. and Mukherjee, P.K. (1992.). Biological seed treatments for control of soil borne plant pathogens. FAO Plant Prot. Bull., 40: 1-10.
Prikryl, Z. and Vancura, V. (1980). Root exudates of plants IV. wheat root exudation as dependent on growth concentration gradient of exudates and the presence of bacteria. Plant and Soil, 57: 69-83.
Rangeshwaran, R. and Prasad, R.D. (2000). Isolation and evaluation of rhizospheric bacteria for biological control of chickpea wilt pathogen. J. Biol. Control., 14: 9-15.
Sakthival, N., Sivamani, E., Unnamalai N. and Gnanamanickam, S.S. (1986). Plant growth-promoting rhizobacteria in enhancing plant growth and suppressing plant pathogens. Curr. Sci., 55(1): 22-25.
Schipper, B., Baker, A.W. and Baker, P.A.H.M. (1987) Interactions of deleterious and beneficial rhizosphere microorganisms and the effect of cropping practice. Ann. Rev. Phytopath., 25: 339-358.
Singh, U.S., Mishra, D.S., Zaidi, N.W., Varshney, S., Sharma, R. and Singh, N. (2005). Potential and effectiveness of fungi and bacteria as biocontrol agents for plant disease management. In Integrated pest management: principles and application Volume 1: Principles (Singh, A., Sharma, O.P. and Garg, D.K. ed.), CBS, New Delhi, India.
Van Peer, R., and Schippers, B. (1988). Plant growth responses to bacterization with selected Pseudomonas spp. strains and rhizosphere microbial development in hydroponic cultures. Can. J. Microbiol., 35: 456-463.
Vidhyasekaran, P. and Muthamilan, M. (1995). Development of formulations of Pseudomonas fluorescens for control of chickpea wilt. Plant Dis., 79: 782-786.
Voisard, C., Keel, C., Hars, D. and Defago, G. (1989). Cyanide production by Pseudomonas fluorescens helps suppress black root rot of tobacco under genotobiotic conditions. EMBO J., 8: 351-358.
Windham, M.T., Elad, Y. and Baker, R. (1986). A mechanism for increased plant growth induced by Trichoderma spp. Phytopathology, 76: 518-521.
Yehia, A.H., EI-Hassan, S. A. and EI-Bahadli, A.H. (1985). Biological seed treatment to control Fusarium root rot of broad bean. Egyptian. J. Phytopathol., 14:59-66.
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Gangwar, G. P. (2013). Growth promotion of rice seedlings by fungal and bacterial bioagents effective against bacterial leaf blight of rice. Journal of Applied and Natural Science, 5(2), 430-434. https://doi.org/10.31018/jans.v5i2.348
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