The aim of present study was to compare the phytochemical composition of different extracts of bee pollen, propolis and honey and to analyse the best solvent for maximum phytochemicals extraction. These preliminary studies are significant in locating the pharmacological bioactive compounds in different extracts of bee products. The tests showed greater number of constituents present in bee pollen and propolis as compared to honey. The concentration of these bioactive constituents was greater in ethanolic extract of propolis and water extract of bee pollen and honey. Ethanolic extract of propolis showed higher concentration of tannins and glycosides. Bee pollen had more concentration of saponins, flavonoids and proteins in water extract. Reducing sugars and carbohydrates were present in larger amount in water extract of honey. These studies will be useful to select the best solvent for honey, pollen and propolis for various research purposes.
Honey, Phytochemical, Pollen, Propolis
Duraipandiyan, V., Ayyanar, M. and Ignacimuthu, S. (2006). Antimicrobial activity of some ethnomedical plants used by Paliyar tribe from Tamil Nadu, India. BMC Complementary and Alternative Medicine, 6: 35.
Ferreira, I.C.F.R., Aires, E., Barreira J. C. M., Estervinho, L.M (2009). Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food Chemistry, 114: 1438-1443.
Harborne , J.B. (1973). Phytochemical Methods, 3th Edn, Chapman and Hall Ltd., London. pp.135-203.
Kalia, P., Kumar, R. K. and Harjai, K. (2013). Phytochemical screening and antibacterial activity of different extracts of Propolis. International Journal of Pharmaceutical and Biological Research, 3:219-222.
Kaur, R., Kumar, N.K. and Harjai, K. (2013). Phytochemical analysis of different extracts of bee pollen. International Journal of Pharmaceutical and Biological Research, 4: 65-68.
Krishnaraju, A. V., Rao, T. V. N. and Sundararaju, D. (2005). Assessment of bioactivity of Indian medicinal plants using Brine shrimp (Artemia salina) lethality assay. Int. J. Appl. Sci. Eng., 2: 125-134.
Kumar, N., Ahmad, M.K.K., Dang, R. and Husain, A. (2008). Antioxidant and antimicrobial activity of Propolis from Tamil Nadu zone. Journal of Medicinal Plants Research, 2 (12): 361-364.
Misra, C. S., Pratyush, K., Dev, M. S. L., James, J., Veettil, A. K. T. and Thankamani, V. (2011). A comparative study on phytochemical screening and antibacterial activity on root of Alstonia scholaris with the roots, leaves and stem bark. Int. J. Res Phytochem. Pharmacol., 1(2): 77-82.
Mohapatra, D.P.,Thakur, V. and Brar, S.K. (2011). Antibacterial efficacy of raw and processed honey. Biotechnology Research International, 1-6.
Nagai,T., Nagashima, T., Myoda, T. and Inoue, R. (2004). Preparation and functional properties of extracts from bee bread. Nahrung/Food, 48 (3): 226 - 229.
Russo, G.L. (2007). Ins and out of dietary phytochemicals in cancer chemoprevention. Biochemical Pharmacology, 74: 533-544.
Sramkova, K.F., Nozkova, J., KaÃ¨Ã¡niovÃ¡, M., MÃ¡riÃ¡ssyovÃ¡, M., RovnÃ¡, K. and StriÃ¨Ãk, M. (2013). Antioxidant and antimicrobial properties of monofloral bee pollen. J. Environ. Sci. Health. B., 48:133-8.
SzczÃªsna, T. (2006). Protein content and amino acid composition of bee collected pollen from selected botanical origins. J. of Api. Sci., 50: 81-90.
Tanaka, H., Sato, M. and Fujiwara, S. (2002). Antibacterial activity of isoflavonoids isolated from Erythrina variegata against methicillin resistant Staphylococcus aureus. Lett. Appl. Microbiol., 35: 494- 498.
Vijayalakshmi, R. and Ravindhran, R. (2012). Preliminary comparative phytochemical screening of root extracts of Diospyrus ferrea (Wild.) Bakh and Aerva lanata (L.) Juss. Ex Schulte Asian Journal of Plant Science and Research, 2 (5): 581-587.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)