Gas chromatography–mass spectrometry (GC-MS) analysis of antimicrobial compounds produced by mahua oil cake against the stem rot pathogen- Sclerotium rolfsii
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Abstract
The antifungal property containing oil cakes play a significant role in reducing plant disease in a wide range of soil-borne pathogens. A destructive soil-borne pathogen, Sclerotium rolfsii is infecting a vast range of crops worldwide. In-vitro efficacy of five different oil cakes viz. mahua cake, neem cake, pungam cake, coconut cake and castor cake extracts was tested against the stem rot pathogen of groundnut. Among the five different oil cakes, mahua oil cake extract produced the minimum mycelial growth of 1.57and1.29 cm at 5%, and 10% concentrations, respectively and showed maximum percent growth inhibition of 83.33and 86.66% respectively. Bioactive compounds in mahua oil cake were analyzed through GC-MS. From the result of GC-MS, the high retention time and peak area percentage were observed with major important bioactive compounds like n-Hexadecanoic acid (24.968) (12.22), Hexadecanoic acid, ethyl ester (23.655) (2.9), 9,12-Octadecadienoic acid (Z, Z)-(28.659)(35.61), 9-Octadecenoic acid, (E)-(28.786) (13.15), Octadecanoic acid (29.137) (33.59) and a1-Octyn-3-ol (3.023) (0.04).The bioactive compounds identified through GC-MS from mahua oil cake extract were found to be exhibiting antifungal activity against S. rolfsii.
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Keywords
GC-MS, Mahua oil cake, Oil cake extract, Sclerotium rolfsii, Stem rot
References
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Akpuaka, A., Ekwenchi, M., Dashak, D., &Dildar, A. (2013). Biological activities of characterized isolates of n-hexane extract of AzadirachtaindicaA. Juss (Neem) leaves. Nature and Science, 11(5), 141-147.
Ali, A., Javaid, A., & Shoaib, A. (2017). GC-MS analysis and antifungal activity of methanolic root extract of Chenopodium album against Sclerotium rolfsii. Planta Daninha, 35.
Alice, D., &Sundravadana, S. (2012). Effects of biocontrol agents and plant products Macrophominaphaseolina and colchicine content in Gloriosa superba. Plant Protection Science, 48(3), 110-115, https://doi.org/10.17221/18/2011-PPS.
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Kumari, N., Menghani, E. &Mithal, R. (2019). GCMS analysis of compounds extracted from actinomycetes AIA6 isolates and study of its antimicrobial efficacy.
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Mubarakali, D., Praveenkumar, R., Shenbagavalli, T., Nivetha, T. M., Ahamed, A. P., Al-Dhabi, N. A., &Thajuddin, N. (2012). New reports on anti-bacterial and anti-candidal activities of fatty acid methyl esters (FAME) obtained from Scenedesmus bijugatus var. bicellularis biomass. RSC Advances, 2(30), 11552-11556.
Pinto, M. E., Araújo, S. G., Morais, M. I., Sá, N. P., Lima, C. M., Rosa, C. A., Lima, L. A. (2017). Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils. Anais da Academia Brasileira de Ciências, 89(3), 1671-1681.
Schmitz, H. (1930). Poisoned food technique. Industrial and Engineering Chemistry-Analytical Edition, 2(4), 361-363.
Sudha, T., Chidambarampillai, S. & Mohan, V. (2013). GC-MS analysis of bioactive components of aerial parts of Kirganeliar eticulata Poir (Euphorbiaceae). Journal of Current Chemical and Pharmaceutical Sciences, 3(2), 113-122.
Vincken, J.-P., Heng, L., De Groot, A. & Gruppen, H. (2007). Saponins, classification and occurrence in the plant kingdom. Phytochemistry, 68(3), 275-297. https://doi.org/10.1016/j.phytochem.2006.1 0.0 08
Wani, S., Narayana, Y. & Rai, P. (1982). Screening Fungicides In-vitro against Fusarium Causing rot of Gladiolus Corms. Indian Journal of Microbiology, 22(1), 49-51.
Xiong, C., Li, Q., Li, S., Chen, C., Chen, Z., & Huang, W. (2017). In vitro antimicrobial activities and mechanism of 1-octen-3-ol against food-related bacteria and pathogenic fungi. Journal of oleo science, ess16196.
Yadav, P. & Singh, D. (2012) Madhuca longifolia (Sapotaceae): a review of its traditional uses, phytochemistry, and pharmacology. Int J. Biomed. Res. 3, 291–305. https://doi.org/10.7439/ijbr.v3i7.292
Agoramoorthy, G., Chandrasekaran, M., Venkatesalu, V., & Hsu, M. (2007). Antibacterial and antifungal activities of fatty acid methyl esters of the blind-your-eye mangrove from India. Brazilian Journal of Microbiology, 38(4), 739-742, https://doi.org/10.1590/S1517-83822007000400028.
Akpuaka, A., Ekwenchi, M., Dashak, D., &Dildar, A. (2013). Biological activities of characterized isolates of n-hexane extract of AzadirachtaindicaA. Juss (Neem) leaves. Nature and Science, 11(5), 141-147.
Ali, A., Javaid, A., & Shoaib, A. (2017). GC-MS analysis and antifungal activity of methanolic root extract of Chenopodium album against Sclerotium rolfsii. Planta Daninha, 35.
Alice, D., &Sundravadana, S. (2012). Effects of biocontrol agents and plant products Macrophominaphaseolina and colchicine content in Gloriosa superba. Plant Protection Science, 48(3), 110-115, https://doi.org/10.17221/18/2011-PPS.
Anitha, K., Thiruvudainambi, S., Harish, S., & Kumar, R. A. (2019). Bio-efficacy of organic amendments and plant oils against Sclerotium rolfsiiSacc.: The incitant of Southern blight of tomato. Journal of Pharmacognosy and Phytochemistry, 8(3), 2202-2205.
Chandrasekaran, M., Kannathasan, K., &Venkatesalu, V. (2008). Antimicrobial activity of fatty acid methyl esters of some members of Chenopodiaceae. Zeitschrift für Naturforschung, 63(5-6), 331-336. https://doi.org/10.1515/znc-2008-5-604.
Dar, W. A., Hassan, M. G., Sheikh, P. A., Summuna, B., &Ganaie, S. A. (2018). Integrated Disease Management Capsule for Wilt/Root Rot Complex of Chili. Int. J. Curr.Microbiol.App. Sci,7(1), 1253-1261.https://doi.org/10.20546/ijcmas.2018.701.152.
Dean, J. R., Abdullah, M. P., & Zakaria, Z. (1997). Extraction of polycyclic aromatic hydrocarbons from contaminated soil using Soxhlet extraction, pressurised and atmospheric microwave-assisted extraction, supercritical fluid extraction and accelerated solvent extraction. Journal of Chromatography, 791(1-2), 361-366, https://doi.org/10.1039/AI9953 200305.
Dubey, S. (2002). Efficacy of some oil cakes and plant extracts against web blight of urd and mung bean caused by Thanatephoruscucumeris. Journal of Mycology and Plant Pathology, 32(2), 158-161.
Frahm, J. P. (2004). Recent developments of commercial products from bryophytes. The Bryologist, 107(3), 277-283.
Gomez, K. A. & Gomez, A. A. (1984). Statistical procedures for agricultural research: John Wiley & Sons.
Jubie, S., Ramesh, P. N., Dhanabal, P., Kalirajan, R., Muruganantham, N., & Antony, A. S. (2012). Synthesis, antidepressant and antimicrobial activities of some novel stearic acid analogues. European Journal of Medicinal Chemistry, 54, 931-935.https://doi.org/ 10.1016/j.ejmech.2012.06.025
Karmelreetha, A. & Muthukumar, A. (2020). Role of oil cakes against soil born pathogens an eco-friendly
approach.
Kumari, N., Menghani, E. &Mithal, R. (2019). GCMS analysis of compounds extracted from actinomycetes AIA6 isolates and study of its antimicrobial efficacy.
Lalitharani, S., Mohan, V., Regini, G. & Kalidass, C. (2009). GC-MS analysis of ethanolic extract of Pothos scandens leaf. J. Herb. Medi. Toxicology, 3, 159-160.
Latha, P., & Rajeswari, E. (2019). Evaluation of biocontrol agents, fungicides and organic amendments against sclerotium wilt (Sclerotium rolfsiiSacc) of jasmine (Jasminum sambac (L.) Aiton). J. Pharmacognosy and Phytochemistry, SP2, 897-902.
Lima, L. a. R. D. S., Johann, S., Cisalpino, P. S., Pimenta, L. P. S., &Boaventura, M. a. D. (2011). In vitro antifungal activity of fatty acid methyl esters of the seeds of Annona cornifolia A. St.-Hil.(Annonaceae) against pathogenic fungus Paracoccidioides brasiliensis. Revista da SociedadeBrasileira de Medicina Tropical, 44(6), 777-780. https://doi.org/ 10.1590/s0037-86822011000600024
Mubarakali, D., Praveenkumar, R., Shenbagavalli, T., Nivetha, T. M., Ahamed, A. P., Al-Dhabi, N. A., &Thajuddin, N. (2012). New reports on anti-bacterial and anti-candidal activities of fatty acid methyl esters (FAME) obtained from Scenedesmus bijugatus var. bicellularis biomass. RSC Advances, 2(30), 11552-11556.
Pinto, M. E., Araújo, S. G., Morais, M. I., Sá, N. P., Lima, C. M., Rosa, C. A., Lima, L. A. (2017). Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils. Anais da Academia Brasileira de Ciências, 89(3), 1671-1681.
Schmitz, H. (1930). Poisoned food technique. Industrial and Engineering Chemistry-Analytical Edition, 2(4), 361-363.
Sudha, T., Chidambarampillai, S. & Mohan, V. (2013). GC-MS analysis of bioactive components of aerial parts of Kirganeliar eticulata Poir (Euphorbiaceae). Journal of Current Chemical and Pharmaceutical Sciences, 3(2), 113-122.
Vincken, J.-P., Heng, L., De Groot, A. & Gruppen, H. (2007). Saponins, classification and occurrence in the plant kingdom. Phytochemistry, 68(3), 275-297. https://doi.org/10.1016/j.phytochem.2006.1 0.0 08
Wani, S., Narayana, Y. & Rai, P. (1982). Screening Fungicides In-vitro against Fusarium Causing rot of Gladiolus Corms. Indian Journal of Microbiology, 22(1), 49-51.
Xiong, C., Li, Q., Li, S., Chen, C., Chen, Z., & Huang, W. (2017). In vitro antimicrobial activities and mechanism of 1-octen-3-ol against food-related bacteria and pathogenic fungi. Journal of oleo science, ess16196.
Yadav, P. & Singh, D. (2012) Madhuca longifolia (Sapotaceae): a review of its traditional uses, phytochemistry, and pharmacology. Int J. Biomed. Res. 3, 291–305. https://doi.org/10.7439/ijbr.v3i7.292
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How to Cite
Gas chromatography–mass spectrometry (GC-MS) analysis of antimicrobial compounds produced by mahua oil cake against the stem rot pathogen- Sclerotium rolfsii. (2022). Journal of Applied and Natural Science, 14(2), 600-606. https://doi.org/10.31018/jans.v14i2.3360
