Pseudomonas aeruginosa has an ability to rapidly develop resistance to most antimicrobial compounds, and to check this ability. The isolates were collected from different pathological human sources and tested for their sensitivity to aminoglycoside antibiotic and to honey, a natural product that is generating renewed interest for its therapeutic application using Kirby Bauer disc diffusion technique. Aminoglycoside antibiotic which is normally active against gram-negative bacteria was used alongside honey. The 29 isolates of P. aeruginosa showed 100%
sensitivity to honey tested in their undiluted form. This was not the case with gentamicin (10?g) and amikacin (30 ?g), both of which varied in their antipseudomonal activity, like even 1:2 aqueous dilution of honey appreciably inhibited pseudomonal isolates than either of the two aminoglycoside antibiotic. Honey is therefore suggested as an effective natural product in overcoming the widespread antibiotic resistance of P. aeruginosa.
Amikacin, Antipseudomonal activity, Gentamicin, Honey
Artz, C.P., Moncrief, J.A. (1969). The treatment of burns. W.B. Saunders Co. Philadelphia: 585.
Basson, N.J. and Grobler, S.R. (2008). Antimicrobial activity of two South African honeys produced from indigenous Leucospermum cordifolium and Erica species on selected micro-organisms. BMC Complement Altern Med, 8: 41.
Bauer, A.W., Kirby, W.M.N., Sherris, J.C. and Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45: 433-496.
Cachia, P.J. and Hodges, R.S. (2003). Synthetic peptide vaccine and antibody therapeutic development: prevention and treatment of Pseudomonas aeruginosa. Biopolymers, 71(2):141-168.
Cooper, R.A., Halas, E., Molan, P.C. (2002). Efficacy of honey in inhibiting strains of Pseudomonas aeruginosa form infected burns wound. Journal of Burn Care Rehabilitation, 23: 366-370.
Cooper, R.A. and Molan, P.C. (1999). The use of honey as an antiseptic in managing Pseudomonas infection. Journal of Wound Care, 8: 161-164.
Cowan, S.T. (1974). Cowan and Steel’s Manual for the Identification of Medical Bacteria 2nd Edinburgh, Cambridge University Press, London pp 1-30.
Hummel, R.P., MacMillan, B.G. and Altemeier, W.A. (1970). Topical and systemic antibacterial agents in the treatment of burns. Annals of Surgery, 172: 370-384.
Irish, J., Blair, S. and Carter, D.A. (2011). The antibacterial activity of honey derived from Australian flora. PLoS One, 6: 182.
Klika, L.J. and Goodman, J.N. (1982). Antibiotic interactions. Journal of American Medical Association, 248: 1309.
Lusby, P.E., Coombes, A.L. and Wilkinson, J.M. (2005). Bactericidal activity of different honeys against pathogenic bacteria. Archives of Medicine Research, 36: 464–467.
Mohr, J.F., Jones, A. and Ostrosky-Zeichner, L. (2004). Associations between antibiotic use and changes in susceptibility patterns of Pseudomonas aeruginosa in a private, university affiliated teaching hospital: an 8-yearexperience: 1995-2002. International Journal of Antimicrobial Agents, 24: 346-351.
Molan, P.C. (1999). The role of honey in the management of wounds. Journal of Wound Care, 8: 415-418.
Osman, O.F., Mansour, J.S. and El-Hakim, S. (2003). Honey compound for wound care: A preliminary report. Annals of Burns and Fire Disasters, 16: 131–134.
Richard, B., Clark, L., Christine, C., Sanders, Cathy, B., Pakiz, Marcia, K. and Hostetter (1988). Aminoglycoside resistance among Pseudomonas aeruginosa isolates with an unusual disk diffusion antibiogram. Antimicrobial Agents and Chemotherapy, 689-692.
Subrahmanyam, M., Shahapure, A.G. and Nagame, N.S. (2001). Effects of topical application of honey on burn wound healing. Annals of Burns and Fire Disasters, 14: 3–5.
Tan, H.T., Rahman, R.A., Gan, S.H., Halim, A.S., Hassan, S.A. and Sulaiman, S.A. (2009). The antibacterial properties of Malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey. BMC Complement Altern Med, 9: 34.
Teplitz, C. (1979). The pathology of burn and fundamentals of burn wound sepsis. In: Artz C.P., Moncrief, J.A., Pruitt, B.A.(Ed’s), Burns: A team approach, W.B. Saunders Co. Philadelphia 45–94.
Todar, K. (2004). Textbook of Bacteriology, Wisconsin- Madison, 1st ed. vol. 11, pp.1425
Tysett, C., de Rautlin, de. La. and Roy, Y. (1993). Assays on the study of osmophilic yeasts, organisms causing fermentation of honey collected in France. Faculty of Pharmacology, University of Nancy Bulletin, 134: 1–26.
Willix, D.J., Molan, P.C. and Harfoot, C.G. (1992). A comparison of the sensitivity of wound infecting species of bacteria to the antibacterial activity of manuka honey and other honey. Journal of Applied Bacteriology, 73: 388-94.
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