Diabetes mellitus is a serious public health problem worldwide. Diabetic foot ulcers (DFU), a major complication in Type 2 diabetes are one of the major causes of morbidity and mortality around the world. To screen various bacterial pathogens present in diabetic foot ulcers and to determine their antibiotic sensitivity to actinomycetes isolated from various fields of Chandragiri, Tirupati, twenty four actinomycetes isolates were isolated and screened by primary and secondary screening methods to determine potent antibiotic producers by using test organisms. Among 24 isolates, 4 were more potent and they showed varied range of antibacterial activity of pathogens, isolated form diabetic foot ulcers. Four isolates were compared with lenezoid antibiotic. Enterococcus was resistant to lenezoid antibiotic but four actinomycetes inhibited the growth of Enterococi.
Actinomycetes, Diabetic foot ulcers, Lenezoid, Antibacterial activity
Bakker, K., Van Houtum, W. H. and Riley, P. C. (2005). The international diabetes federation focuses on the diabetic foot. Curr Diab Rep, 5: 436-440.
Dhananjeyan, V., Selvan, N. and Dhanapal, K. (2010). Isolation, characterization and antibiotic sensitivity of actinomycetes from Locally (Near MCAS) collected soil samples. Journal of Biological Sciences, 10 (6): 514-519.
Edmonds, M.E. and Foster, A. (2004). The use of antibiotics in the diabetic foot. Am. J. Surg., 187: S25-S28.
Johnson, L.F. and Curl, E.A. (1972). Methods for research on the ecology of soil-borne plant pathogens. Burgess Publishing Company, Minneapolis. 247pp.
Lacey, J. (1973). Actinomycetales: Characteristics and practical Importance. Edited by Sykes, G. and Skinner, F. The society for Bacteriology Symposium Series, Academic press London- New York No.2.
Mc-Carthy, A.J. and Williams, S.T. (1990). Methods for studying the ecology of Actinomycetes. Methods in Microbiology. Ed. By Grigorova and Norris, J.R. Academic Press Limited. London, 22:533-363.
Naikpatil, S.V. and Rathod, J.L. (2011). Selective isolation and antimicrobial activity of rare actinomycetes from mangrove sediment of Karwar. Journal of Ecobiotechnology, 3 (10): 48-53.
Oskay, M., Tamer, A.U., and Azeri, C. (2004). Antibacterial activity of some actinomycetes isolated from forming soils of Turkey. African Journal of Biotechnology, 3: 441-446.
Ouhdouch, Y., Barakate, M. and Finanse, C. (2001). Actinomycetes of Moroccan habitats: Isolation and screening for antifungal activities. Eur. J. Soil Biol., 37: 69-74.
Ozer, B., Kalaci, A., Semerci, E., Duran, N., Davul, S., and Yanat, A.N. (2010). Infections and aerobic bacterial pathogens in diabetic foot. African Journal of Microbiology Research, 4: 2153-2160.
Pathare ,N. A., Bal, A., Talvalkar, G.V. and Antani, D.U. (1998). Diabetic foot infections: a study of microorganisms associated with the different Wagner gardens. Indian J.Pathol.Mocrobiol., 41:437-441.
Saadoun, I. and Gharaibeh, R. (2003). The Streptomyces flora of Badia region of Jordan and its potentials as a source of antibiotics active against antibiotic-resistant bacteria. J.Arid. Environ., 53:365-371.
Sen., K.S., Haque, F.S. and Pal, C.S. (1995). Nutrient Optimization for production of broad spectrum antibiotics by Streptomyces. Antibiotics Str. 15.4. Acta. Microbial. Hung., 42:155-162.
Siva Kumari, V. and Shanthi, G. (2009). Antibiotic susceptibility of common bacterial pathogens isolated from diabetic pus. Advanced Biotech., 10-13.
Tara Devi, G., Sherpa, C., Viswanath, P.A. and Bind, L. (2009). Isolation and characterization of antibacterial actinomycetes from soil samples of Kalapattar, Mount Everest Region. Nepal Journal of Science and Technology, 10:173-182.
Westly, J.W., Ecans, R.H., Sello, R.H., Troupe, N., Liu, C.M., Blount, J.F. (1986). Isolation and characterization of antibiotic X-1454 A, a novel monocarboxylic acid ionophore produced by Streptomyces antibioticus NRRL 18167. J. Antibioti. Tokyo, 32(2):100-107.
Yoga, R., Khairul, A. Sunita, K. and Suresh, C. (2006). Bacteriology diabetic foot lesions. Med. J. Malaysia, 61:6-14.
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