B. Vineela Deborah R. Jaya Madhuri


The laboratory studies were conducted to resolute the effects of Imidacloprid (insecticide) and Triadimefon (fungicide) singly and in combination on enzymatic activities of soil microorganisms in tomato cultivated soils at different concentrations of 0.2, 0.5 and 0.7 kg per hectare. It was observed that phosphatase, protease and urease activities were elevated noteworthy at 0.5kg per hectare after 10 days of incubation. At lower concentration the activities of these enzymes showed no significant difference from that of control. The combination of the two pesticides led to a pronounced decline of phosphatase and protease enzyme activities at higher concentration. Triadimefon had no effect on urease activity at low concentrations whereas at 0.5 and 0.7 concentrations there was a significant increase when compared to control.


Download data is not yet available.


Metrics Loading ...




Imidacloprid, Soil enzymes, Tomato cultivated soil, Triadimefon

Fawcett J. and Scot, J.E. (1960). A rapid and precise method for determination of urea. J. Clin. Path., 13:156-159
Ingram, C.W., Coyne, M.S. and Williams, D.W. (2005). Effects of commercial diazonin and imidacloprid on microbial urease activity in soil and sod. Journal of Environmental Quality, 34:1573-1580.
Jackson, M.L. (1971). Soil chemical analysis. Prentice Hall. New Delhi.
Jaya Madhuri, R. and Rangaswamy.V. (2009). Effect of selected insecticides on microbial activities of dehydrogenase, protease and urease in groundnut soils. Indian Journal of Environmental Sciences,13(1):1-8.
Kumar, J.K. and Prakash, J. (1993). Persistence of thiobencarb and butachlor in soil incubated at different temperatures. In: Integrated weed management for sustainable agriculture. Proceedings of Indian Society of Weed Science International Seminar. Hisar, India. pp. 123–124.
Kandeler, E. (1989). Protease in soils and methods from the determination of their activity. Soils and Fertilizers, 6:68-72
Ladd, J.N. and Butler, J.H.A. (1972). Short-term assays of soil proteolytic enzyme activities using proteins and dipeptide derivatives as substrates. Soil Biol. Biochem., 4: 19-30.
Nannipieri, P., Giagnoni,L., Landi,L., and Renella,G. (2011). Role of Phosphatase Enzymes in Soil. Soil Biology, 100(2): 215-243.
Nasreen, C., Jaffer Mohiddin, G., Srinivasulu, M., Rekhapadmini, A., Ramanamma,P., and Rangaswamy, V. (2012). Interaction effects of insecticides on enzyme activities in black clay soil from groundnut (Arachis hypogeal.L.) fields). Environmental Research, Engineering and Management. 31(2). 21-28.
Omar, S.A. and Abdel Sater, M.A. (2001). Microbial populations and enzyme activities in soil treated with pesticides. Water, Air and Soil Pollution, 127(1-4): 49-63.
Pahwa, S.K. and Bajaj, K. (1999). Effects of pre-emergence Herbicides on the activity of á- amylase and protease enzyme during germination in Pigeonpea and Carpetweed. Indian Journal of Weed Science,31(3-4):148-150.
Ramudu, A.C., Madakka, M., Srinivasulu, M. (2011). Impact of fungicides chlorothalonil and propiconazole on microbial activities in groundnut (Arachis hypogeal L.)soils.ISRN Microbiology, Volume 2011 (2011), Article ID 623404.
Sannino, F. and Gianfreda, L. (2001). Pesticide influence on soil enzyme activities. Chemosphere, 45: 417 -425.
Tabatabai (1983). Soil enzymes, Methods of soil analysis. Part 2. Microbiological and biochemical properties. Soil. Biol. Biochem 15:403–408, 1983); Soil Science Society of America (1994), Madison, pp 775–833.
Walkley, A. and Black, I.A. (1934). An examination of the degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci., 37: 29-38.
Zhang WenJun, FuBin Jiang and JianFeng Ou. ( 2011). Methods of soil and agro chemistry analysis. Chinese Agricultural Science and Technology Press, Beijing (in Chinese). 156 No tillage affects soil enzymes kinetics.
Citation Format
How to Cite
Deborah, B. V., & Madhuri, R. J. (2013). Effect of Imidacloprid and Triadimefon on microbial phosphatase, protease and urease enzyme activities in tomato (Lycopersicon esculentum sp.) cultivated soil. Journal of Applied and Natural Science, 5(2), 323-327. https://doi.org/10.31018/jans.v5i2.325
More Citation Formats:
Research Articles