##plugins.themes.bootstrap3.article.main##

Usha S. S. Remya Krishnan Murugan K.

Abstract

Dehydration and rejuvenation during rehydration is the salient feature of certain plants which can withstand drought. The present study was undertaken to justify the tolerance capacity of Campylopus flexuosus, the moss of the Ponmudi belts of Thiruvananthapuram, against dehydration followed by rehydration. Fresh leafy plants of C. flexuosus were hydrated, afterwards dried, and rehydrated under in vitro environment. In the course of loss of water from cells, the relative water content of desiccated thallus was reduced after 4 h with intense inward curling. Upon rehydration, the RWC was regained 85% of its initial water content within hours. The rehydrated thallus showed the normal morphology. Photosynthetic parameters like chlorophyll b (1.01 to 1.56 μg g –1 ), and total carotenoid (0.251 to 0.514 μg g –1 ) increased remarkably in the desiccated state. Superoxide radical (O2 _) content increased (11.4 nmol/g FW), resulting in an oxidative burst during desiccation. Consequently, antioxidant enzymes such as catalase (0.369 U mg protein −1), superoxide dismutase ( 2.68 to 6.02 Units mg−1), peroxidase ( 0.12 μmol min−1 g−1 protein) and glutathione reductase ( 312 Units mg−1 protein) activities were up-regulated in the desiccated thallus to ameliorate oxidative damage. Increased malondialdehyde (1.08 nmol g−1 FW) content during desiccation substantiates membrane damage and loss of its integrity. During desiccation, the osmolytes sucrose and proline (27.6 and 2.57 μmol/g FW respectively) were enhanced to maintain cell structure integrity. After rehydration, biochemical and morphological properties were maintained similar to hydrated conditions. Thus, the study reflects the unique adaptations of the moss to tide over desiccation tolerance.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

##plugins.themes.bootstrap3.article.details##

##plugins.themes.bootstrap3.article.details##

Keywords

Antioxidant enzymes, Desiccation, Photosynthetic pigments, Proline, Resurrection, Tolerance

References
Abbasi, H., Jamil, M., Haq, A., Ali, S., Ahmad, R., Malik, Z.& Veen, P. (2016). Salt stress manifestation on plants, mechanism of salt tolerance and potassium role in alleviating it: a review. Zemdirbyste Agriculture 103, 229 -238.
Acosta-Motos, J.R., Ortuño, M.F., Bernal-Vicente, A., Diaz-Vivancos, P., Sanchez Blanco, M.J. &Hernandez J.A. (2017). Plant responses to salt stress: adaptive mechanisms. Agronomy, 7, 3390.
Ahanger, M.A., Siddique, K.H.M. & Ahmad, P. (2021). Understanding drought tolerance in plants. Drought Tolerance e172 (2), 286-288
Al  Hassan, M., Chaura, J., Donat-Torres, M.P., Boscaiu, M.& Vicente, O. (2017). Antioxidant responses under salinity and drought in three closely related wild monocots with different ecological optima. AoB Plants 1–20.
Allel,  D., BenAmar, A., Badri, M. &Abdelly  C. (2019). Evaluation of salinity tolerance indices in North African barley accessions at reproductive stage. Czech Journal of Genetics and Plant Breeding 55, 61–69.
Almeselmani, M., Deshmukh, P. S. & Sairam, R. K. (2009). High temperature stress tolerance in wheat genotypes: role of antioxidant defence enzymes. Acta Agronomica Hungarica. 57: 1–14.
Banupriya, T .G., Devaraja Akash, C. R., Yathish, N. S. & Sharthchandra, R. G .(2020). Studies on the mechanism of desiccation tolerance in the resurrection fern Adiantum raddianum Journal of Applied Biology & Biotechnology Vol. 8(01), 6-14,
Behrouzi, M., Valizadeh, M. & Vahed, M.M. (2015). Catalase and peroxidase antioxidant enzyme activities in barley cultivars seedling under salt stress. Bulletin of Environmental Pharmacology Life Science 4, 29–35.
Ben  Chikha, M., Hessini, K., Ourteni, R.N., Ghorbel,  A& Zoghlami, N. (2016). Identification of barley landrace genotypes with contrasting salinity tolerance at vegetative growth stage. Plant Biotechnology 33:287–295.
Ben Khaled, A., Hayek, T., Mansour, E., Hannachi, H., Lachiheb, B. & Ferchichi, A. (2012). Evaluating Salt tolerance of 14 barley genotypes from southern Tunisia using multiple parameters. Journal of Agriculture Sciences 4, 27–38.
Bornare, S.S., Prasad, L.C. & Kumar, S. (2013). Comparative study of biochemical indicators of salinity tolerance of barley (Hordeum vulgare L.) with other crops: a review. Canadian Journal of Plant Breeding 1:97–102.
Burnett, A.C., Serbin, S.P., Davidson, K.J., Ely, K.S. & Alistair Rogers. (2021). Detection of the metabolic response to drought stress using hyperspectral reflectance. Journal of Experimental Botany 72(18), 6474 –6489. https://doi.org/10.1093/jxb/erab255
Change, B. & Maethly, A.C., (1995). Assay of catalases and peroxidase. Methods in Enzymology, 2, 764–775.
Das, K. & Roychoudhury, A. (2014). Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science 2,53.
de Carvalho,R.,  Maurício,  A., Franscisco Pereira,  M., Marques da Silva, J. & Branquinho, C. (2019). All for One: The Role of Colony Morphology in Bryophyte Desiccation Tolerance. Frontiers in Plant Science. 10:1360-1368
Deeba, F., Pandey, V., Pathre, U. & Kanojiya, S. (2009). Proteome analysis of detached fronds from a resurrection plant Selaginella bryopteris- response to dehydration and rehydration. Journal of Proteomics and Bioinformatics, 2,108–16.
Desoky El-Sayed, M., Mansour Elsayed, El-Sobky, El-Sayed, E.A., Abdul-Hamid Mohamed, I., Taha Taha, F., Elakkad Hend, A.., Arnaout Safaa, M. A. I., Eid Rania, S. M., El-Tarabily Khaled, A. & Yasin Mohamed A.T. (2021). Physio-biochemical and agronomic responses of faba beans to exogenously applied nano-silicon under drought stress conditions. Frontiers in Plant Science 12, 1902-1910 DOI=10.3389/fpls.2021.637783
Farrant, J. M., Lehner, A., Cooper, K. & Wiswedel, S. (2009). Desiccation tolerance in the vegetative tissues of the fern Mohria caffrorum is seasonally regulated.The Plant Journal 57, 65–79. doi: 10.1111/j.1365-313X.2008.03673.x
Fontana, L.M.&Rosei, M.A. (2001). Interaction of enkephalins with oxyradicals. Biochemical Pharmacology, 61 (10),1253–7
Gao, B., Zhang, D., Li, X.,  Yang, H., Liang, Y., Chen, M.,  Zhang, Y., Zhang, J. & Andrew, W. (2018). Desiccation tolerance in bryophytes: the rehydration proteomes of Bryum argenteum provide insights into the resuscitation mechanism. Journal of Arid Land, 10, 152–167. https://doi.org/10.1007/s40333-017-0033-3
Halliwell, B. & Foyer, C.H. (1978). Properties and physiological function of a glutathione reductase purified from spinach leaves by affinity chromatography. Planta,139, 9–17.
Hasanuzzaman, M., Nahar, K., Alam, M.M., Roychowdhury, R.& Fujita, M. (2013). Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. International Journal of Molecular Sciences 14,9643–9684
Kavitha, C. H.& Murugan. K. (2016). Dissimilitude response of peroxidases of Dicranopteris linearis (Burm.F.) Underw. against desiccation and rehydration stress. IOSR Journal of Biotechnology and Biochemistry; 2, 36–41
Khalvandi, M., Siosemardeh, A., Roohi, E. & Keramati, S. (2021). Salicylic acid alleviated the effect of drought stress on photosynthetic characteristics and leaf protein pattern in winter wheat. Heliyon, 7(1), e05908. https://doi.org/10.1016/j.heliyon.2021.e05908
Li, H.S., Sun, Q., Zhao, S.J., Zhang, W.H. (2000).In Principles and Techniques of Plant Physiological Biochemical Experiment. Higher Education Press Beijing, China.
Lubaina, A.S., Brijithlal, N.D.& Murugan. K. (2016).Unravelling desiccation and rehydration tolerance mechanism in the fern, Adiantum latifolium. Bioscience Biotechnology Research Communications, 9(4), 672–9.
Pandey, V., Ranjan, S., Deeba, F., Pandey, A.K., Singh, R., Shirke, P.A. & Pathre, U.V. (2010). Desiccation-induced physiological and biochemical changes in resurrection plant, Selaginella bryopteris. Journal of Plant Physiology, 167, 1351–1359
Porra, R.J., Thompson, W.A. & Kriedemann, P.E. (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta  (BBA)-Bioenergetics.975(3),384–94.
Wang, X., Chen, S., Zhang, H., Shi L., Cao. F, Guo .L.(2010).Desiccation tolerance mechanism in resurrection fern-ally Selaginella tamariscina revealed by physiological and proteomic analysis. Journal of Proteome Research 9, 6561–77.
Wood, A. J. (2007).The nature and distribution of vegetative desiccation-tolerance in hornworts, liverworts and mosses. Bryologist, 110, 163–177.
Yathisha, N. S., Barbara,P., Gügi,B., Yogendra, K ., Jogaiah,S., Azeddine,D.& Sharatchandra, R. G. (2020). Vegetative desiccation tolerance in Eragrostiella brachyphylla: biochemical and physiological responses Heliyon, 6 e04948
Zhang, Y., Li  ,Z., Peng  ,Y., Wang , X., Peng , D., Li , Y., He,  X., Zhang,  X., Ma , X., Huang, L.& Yan,Y. (2015). Clones of FeSOD, MDHAR, DHAR genes from white clover and gene expression analysis of ROS-scavenging enzymes during abiotic stress and hormone treatments. Molecule, 20, 20939–20954.
Citation Format
How to Cite
S. S., U. . ., Krishnan, R. ., & K. , M. . . (2021). Desiccation-resurrection linked antioxidant machinery of a moss species Campylopus flexuosus (Hedw.) Bird . Journal of Applied and Natural Science, 13(4), 1407–1413. https://doi.org/10.31018/jans.v13i4.3112
More Citation Formats:
Section
Research Articles