Somenath Kundu Amalendu Sinhababu


Leaf cuticle was covered by epicuticular wax consisting mainly of straight chain aliphatic hydrocarbons with a variety of substituted groups. The studies of cuticular characters of leaves had played an important role in chemotaxonomy. An n-hexane extract of fresh and mature leaves of Ficus glomerata, containing a thin layer of epicuticular waxes was analyzed for the first time by Thin Layer Chromatography, Infrared Spectroscopy, Gas Chromatography and Scanning Electron Microscopy using standard hydrocarbons. The leaves contained 18 identified long chain (C15–C33) n-alkanes except C23 accounting for 68.82% of the hydrocarbons, and an unknown number of unidentified branched chain alkanes. The predominant n-alkanes were C16 (5.92%), C17 (6.18%), C27 (5.11%), C29 (5.29%), C31 (5.47%), whilst C15 (4.21%), C18 (4.57%), C26 (3.88%), C28 (3.53%), C30 (3.43%) n-alkanes were moderately abundant. The C19 (2.53%), C20 (2.52%) and C22 (2.16%) homologues were present only in minor amounts. SEM views were also taken for epicuticular layers and hydrocarbons of the leaves. Qualitative and Quantitative characterization of n-alkanes present in the epicuticular wax can be used as an effective tool in chemotaxonomical work.


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Epicuticular leaf waxes, Ficus glomerata Roxb., GC, n-Alkanes, SEM

Ali, M. and Qadry, J.S. (1987). Amino acid composition of fruits and seeds of medicinal plants. Journal of Indian Chemical Society, 64: 230–231.
Baker, E. A. (1982). Chemistry and Morphology of Plant epicuticular waxes. In Cutler D.F., Alvin K.L., and Price C.E. (eds.), The Plant Cuticle. Academic Press, London. 139-165.
Baruah, K.K. and Gohain, A.K. (1992). Chemical composition and nutritive value of Dimaru (Ficus glomerata Roxb.) leaves. Indian Journal of Animal Nutirition, 9: 107–108.
Castillo, J.B.D., Brooks, C.J.W., Cambie, R.C., Eglinton, G., Hamilton, R.H. and Pellitt, P. (1987). The Taxonomic distribution of some hydrocarbons in gymnosperms. Phytochemistry, 6: 391-398.
Chopra, R.N., Nayar, S.L. and Chopra, I.C. (2002). Glossary of Indian Medicinal Plants. Council of Scientific and Industrial Research, NISCAIR, New Delhi. 199.
Chowdhury, N., Ghosh, A., Bhattacharjee, I., Laskar, S. and Chandra, G. (2010). Determination of the n-alkane profile of epicuticular wax extracted from mature leaves of Cestrum nocturnum. Natural Product Research, 24: 1313-1317.
Dyson, W.G. and Herbin, G.A. (1970). Variation in leaf wax alkanes in Cypress tress grown in Kenya. Phytochemistry, 9: 585-589.
Hegnauer, R. (1986). Phytochemistry and plant taxonomy-an essay on the chemotaxonomy of higher plants. Phytochemistry, 25(7):1519-1535.
Jetter, R. and Schaffer, S. (2001). Chemical composition of Prunus laurocerasus leaf surface. Dynamic Change of the leaf epicuticular wax film during leaf development. Plant Physiology, 126: 1725-1737.
Khan, N. and Sultana, S. (2005). Chemomodulatory effect of Ficus racemosa extract against chemically induced renal carcinogenesis and oxidative damage response in Wister rats. Life Science, 77: 1194–1210.
Kolattukudy, P.E. (1996). Biosynthetic pathways of cutin and waxes, and their sensitivity to environmental stresses. In Kerstiens, G. (eds.), Plant Cuticles. BIOS Scientific publishers Ltd., Oxford. 83.
Lerman, S.L. and Darley E.F. (1975). Particulates. In Kozlowski T.T. (eds.), Responses of plants to air pollution. Academic Press, New York. 141-158.
Mishra, V., Khan, N.U. and Singhal, K.C. (2005). Potential anti-filarial activity of fruit extracts of Ficus racemosa Linn. against Setaria cervi in vitro. Indian Journal of Experimental Biology, 43: 346–350.
Paarakh, PM. (2009). Ficus racemosa Linn.-An overview. Natural Product Radiance, 8: 84-90.
Piasentier, E., Bovolenta, S. and Malossini, F. (2000). The n-alkane concentrations in buds and leaves of browsed broad leaf trees. Journal of Agricultural Science, 135: 311-320.
Prasad, M.S.V. and Inamdar, J.A. (1990). Effect of cement kiln dust pollution on black gram Vignamungo (L.) Hepper. Proceedings of Indian Academy of Sciences (Plant Sciences), 100: 435-443.
Prasad, M.S.V., Subramanian, R.B. and Inamdar, J.A. (1991). Effect of cement kiln dust on Cajanuscajan (L.) Millsp. Indian Journal Environmental Health, 33: 11-21.
Ramasamy, D., Rampratap, M. and Khan, S.A. (2011). Comparative studies on fruits of two Ficus species using HPTLC and quality control methods. Journal of Pharmacy Research, 4(11): 4106-4108.
Rao, Ch.V., Verma, A.R. and Vijayakumar, M. (2008). Gastroprotective effect of standardized extract of Ficus glomerata fruit on experimental gastric ulcers in rats. Journal of Ethnopharmacology, 115: 323–326.
Ripu, M., Kunwar, I.and Rainer, W.B. (2006). Ficus (Fig) species in Nepal review of diversity and indigenous uses. Journal of Applied Ecology, 11(1): 85-87.
Saber, M., Kashmiri, M.A., Mohy-ud-din, A., Ahmed, M. and Khanum, R. (2010). Epicuticular wax of Tamarix aphylla L. Journal of Chemical Society Pak, 32(5): 662-667.
Singh, B.D. and Chaudhary, R.B. (1985). Principle of biomedical and statistical analysis, Kalyani Publishers.
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Kundu, S., & Sinhababu, A. (2013). Analysis of n-alkanes in the cuticular wax of leaves of Ficus glomerata Roxb. Journal of Applied and Natural Science, 5(1), 226–229. https://doi.org/10.31018/jans.v5i1.311
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