A comparison of mutagenic potential of Aji-no-Moto with a traditional chemical mutagen on microsporogenesis in barley (Hordeum vulagre L.)
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Abstract
Aji-no-Moto or Mono Sodium Glutamate (MSG) is a flavour enhancer being used extensively in South East Asian cuisine. The Federation of American Societies for Experimental Biology for the United States Food and Drug Administration (FDA) has concluded that MSG is safe when "eaten at customary levels" but there is still great confusion regarding its toxicity at higher concentrations. Therefore, it was decided to assess the mutagenic efficacy of MSG on a plant system and present the findings as a model for probably similar effects in the animal model. For this, a traditionally popular genus for genetic studies, Hordeum vulgare L. or winter barley, was used as the model system. The studies of microsporogenesis were done in order to see the long term effect. The sets were compared with experimental sets of plants grown from seeds treated with a traditional chemical mutagen Ethyl Methane Sulphonate (EMS). The study revealed that MSG does not induce much genotoxic effects at lower doses and the chromosomal damages induced were very few. However, at higher doses, it almost equals the effects of EMS in terms of heritable genetic damage. The work is significant as MSG continues to be one of the most popular flavouring agents and does not face any challenge to its biosafe status. However, the clastogenic and chromotoxic effects of higher doses of MSG as observed in the study are in total contradiction to the popular belief.
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Article Details
Aji-no-moto, Barley, Chromosomal aberrations, EMS, Genotoxic, Monosodium glutamate
Baker, J. R. (1958). Principles of biological microtechnique. - London and New York.
Battaglia FC. (2000). Glutamine and glutamate exchange between the fetal liver and the placenta. J. Nutrition 130, 974-977.
Bodmer, W. F. & Parsons, P. A. (1962). Linkage and recombination in evolution. - Adv. Genet. 11, 1-100.
Borah S.P. & Talukdar J. (2002). Studies on the cytotoxic effects of extracts of castor seed (Ricinus communis L.). Cytologia. 67, 235-243.
Chauhan N. & Chauhan A.K.S. (1999). Genotoxicity of fluoroquinolones in Allium cepa test system. J. Cytol Genet. 34, 153-160.
El-Ghamery A.A., El-Kholy M.A. & Abou El-Yousser, M. A. (2003). Evaluation of Cytological effect of Zn2+ in relation to germination and root growth of Nigella sativa L. and Triticum aestivum L. Mut. Res. Genet. Toxic.& Environ. Mut. 537, 29-40.
EUFIC (2002). “The facts on Mono Sodium Glutamate”. The European Union Food Information Council. 03 July 2002. (https,//www.eufic.org/en/whats-in-food/article/the-facts-on-monosodium-glutamate)
Gaul, K. Bender, K., Ulonaska, E. & Sato, M. (1966). EMS induced genetic variability in barley, the problems of EMS induced sterility and method to increase the efficiency of EMS treatment in mutation breeding, IAEA, Vienna, 63-64.
Gov. of Canada (2008). "Monosodium glutamate (MSG) – Questions and Answers". Government of Canada. 29 Jan 2008. (https,//www.canada.ca/en/health-canada/services/food-nutrition/food-safety/food-additives/monosodium-glutamate-questions-answers.html)
Hayward, T. (2016). OMG I love MSG. Financial Times. Nikkei.
Hoda A. Khatab & Nagat S. Elhaddad. (2015). Evaluation of Mutagenic Effects of Monosodium Glutamate using Allium cepa and antimutagenic action of Origanum majorana L. and Ruta chalepensis Medical Plants. British Biotechnology Journal. 8(1), 1-11.
Ikeda, K. (2002). New seasonings. Chem. Senses. 27 (9), 847-849.
Jones, G. H. (1987). Chiasmata. -In, Meiosis (ed P. B. MOENS), Academic Press, London, p. 213-244.
Khan, I. A. (1996). Meiotic irregularities induced by di ethyl sulphate (DES) in chilli pepper (Capsicum annum L.) var. NP 46 A. Prog. Hort. 28 (1-2), 36-40.
Kumar L.P. & Panneerselvam N. (2007). Cytogenetic studies of food preservative in Allium cepa root meristem cells. Med. Biol. 14(2),60-63.
Lal J. & Srinivasachar D. (1979). Induction of segmental interchanges in pearl millet (Pennisetum typhoides). Theoretical and Applied Genetics 54, 27–32.
Loliger J. 2000. Function and importance of Glutamate for Savory Foods. J. Nutrition 130 (4th Suppl), 915–920.
Mitra, P.K. & Bhowmik, G. (1996). Cytological abnormalities in Nigella sativa induced by gamma rays and MS. J. Cytol. Gent. 31 , 205 – 215.
Nagwa R.A., Magda A.M., Atef A.A., Elham A.A. (2011). Relative Mutagenecity of Some Food Preservatives on Plant Cells. Aust. J. Basic Appl. Sci. 5(12), 2817-2826.
Naseem S. & Kumar G. (2012). Induced desynaptic variation in poppy (Papaver somniferum L.). Crop Breeding and Applied Biotechnology 13, 363-366.
Kamal N., Elizabeta Z. & Jonathan, S. (2018). Extensive use of Monosodium Glutamate, A Threat to Public Health? EXCLI Journal. 17, 273-278.
Patil B.C. & Bhat G.I. (1992). A comparative study of MH and EMS in the induction of chromosomal aberration on lateral root meristem in Clitoria tematea L. Cytologia. 57,295–264.
Patil, S.H. & Bora, K.C. (1961). Meiotic abnormalities induced by X –rays in Arachis hypogea. Ind. J. Genet. 21, 58 – 61.
Rao, N. B. & Laxmi, N. (1980). Gamma ray induced meiotic abnormalities in Capsicum annum L. Caryologia 33, 509-518.
Renjana P.K., Anjana S. & Thoppil E. John. (2013). Evaluation of genotoxic effects of baking powder and monosodium glutamate using Allium cepa assay. Int. J. Pharmacy & Pharmaceutical Sci. 5 (2), 311-316.
Sato M. & Gaul H. (1967). Effects of ethylmethane sulphonate on the fertility of barley. Rad. Bot. 7, 7-15.
Sinha S.K. & Mohapatra B.K., (1969). Compensatory chiasma formation in maize. Cytologia 34,523–527
Sinha, S. S. N. & Roy. H. (1976). Distribution of chiasmata between and within nuclei in irradiated and non- irradiated population in Phaseolus sp. J. Cytol. Genet. 11, 7-9.
Sree Ramulu, K. (1971). Effects of ionizing radiation and chemical mutagens on chiasma frequency in Sorghum. Cytologia 36, 543-541.
Tarar, J. L. & Dnyansagar, V. R. (1980). Comparison of ethyl methane sulphonate and radiation induced meiotic abnormalities in Turnera ulmifolia Linn. var. angustifolia wild. Cytologia 45, 221-231.
Turkoglu S. (2007). Genotoxicity of five food preservatives tested on root tips of Allium cepa L. Mut. Res. 626, 4–14.
USFDA "Questions and Answers on Monosodium glutamate (MSG)". U.S. Food and Drug Administration. 19 Nov. 2012. (https,//www.fda.gov /food/food-additives-petitions/questions-and-answers-monosodium-glutamate-msg)
Walker R. & Lupien J.R. (2000). The Safety Evaluation of Monosodium Glutamate. The J. Nutrition. 130(4), 1049S–1052S.
Yamaguchi, S. (1991). Basic properties of umami and effects on humans. Physiology & Behavior 49 (5), 833–41.
Young S.W. & Young P.W. (1993). Effect of plant growth regulators on mitotic chromosomes. The Nucleus. 36, 109-113.
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