M. Ayyandurai R. Akila K. Manonmani M. Theradimani S. Vellaikumar


Groundnut (Arachis hypogaea L.) suffers from many soil borne pathogens that deteriorate the quality of the seeds and are responsible for high yield loss. Practically Trichoderma sp. is used for seed treatment, it minimizes the seed and soil borne pathogens and supports plant growth promotion activities. In the present study, five different isolates of Trichoderma spp. were isolated from groundnut (A. hypogaea ) rhizosphere soil. All the five isolates were confirmed by morphological methods and using molecular tools through Polymerase Chain Reaction (PCR) amplification of Internal Transcribed Spacer (ITS) region of Trichoderma sp. and DNA gets amplified in 650 bp to 700 bp. Trichoderma spp. were molecularly identified as T(SP)-20 (Trichoderma longibrachiatum), T(AR)-10 (T. asperellum), T(VT)-3 (T. hamatum), T(BI)-16 (T. longibrachiatum), T(TK)-23 (T. citrinoviride). Phytostimulation activities of all the six isolates viz., phosphate solubilization, Ammonia production, IAA production, and Siderophore production, were evaluated. Among the six isolates, T(SP)-20, T(AR)-10, and TNAU-TA showed higher phytostimulation activities. The growth promotion of Trichoderma spp. on groundnut was assessed through the roll towel method. The isolate T(SP)-20 (T. longibrachiatum) produced the highest germination percentage of 93.33 and vigor index of 2246.2. This work developed a new isolate of T. longibrachiatum (T(SP)-20) which is a native isolate having significant  phytostimulation and growth promotion activities and it could be exploited for other soil borne disease managing successfully.


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Groundnut, Growth promotion, Sclerotium rolfsii, Soil-borne pathogens, Trichoderma spp.

Agrawal, D. P. K. & Agrawal, S. (2013). Characterization of Bacillus sp. strains isolated from rhizosphere of tomato plants (Lycopersicon esculentum) for their use as potential plant growth promoting rhizobacteria. Int. J. Curr. Microbiol. App. Sci, 2(10), 406-417.
Azarmi, R., Hajieghrari, B., & Giglou, A. (2011). Effect of Trichoderma isolates on tomato seedling growth response and nutrient uptake. African journal of Biotechnology, 10(31), 5850-5855. https://doi.org/10.5897/AJB10.1600
Castle, A., Speranzini, D., Rghei, N., Alm, G., Rinker, D., & Bissett, J. (1998). Morphological and molecular identification of Trichoderma isolates on North American mushroom farms. Applied and Environmental Microbiology, 64(1), 133-137.
Carvalhais, L. C., Dennis, P. G., Badri, D. V., Kidd, B. N., Vivanco, J. M. & Schenk, P. M. (2015). Linking jasmonic acid signaling, root exudates, and rhizosphere microbiomes. Molecular Plant-Microbe Interactions, 28(9), 1049-1058.
Contreras-Cornejo, H. A., Macías-Rodríguez, L., López-Bucio, J. S., & López-Bucio, J. (2014). Enhanced plant immunity using Trichoderma. In Biotechnology and Biology of Trichoderma (pp. 495-504): Elsevier. https://doi.org/10.1016/B978-0-444-59576-8.00036-9
Dye, D. (1962). The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. New Zealand Journal of Science, 5(4).
Elad, Y., & Chet, I. (1983). Improved selective media for isolation ofTrichoderma spp. orFusarium spp. Phytoparasitica, 11(1), 55.
Gomez, K. A., & Gomez, A. A. (1984). Statistical procedures for agricultural research: John Wiley & Sons.
Gordon, S., & Paleg, L. (1957). Quantitative measurement of indole acetic acid. Physiol Plant, 10, 37-48.
Guey, N., Kumar, K., Dangue, A., & Arama, M. (2018). Bioproduction of indol 3 acetic acid by Trichoderma strains isolated from agriculture field soils in Senegal. World J Pharmaceutical Res, 7(17), 817-825.
Halifu, S., Deng, X., Song, X., & Song, R. (2019). Effects of two Trichoderma strains on plant growth, rhizosphere soil nutrients, and fungal community of Pinus sylvestris var. mongolica annual seedlings. Forests, 10(9), 758.
Hartmann, A., Singh, M., & Klingmüller, W. (1983). Isolation and characterization of Azospirillum mutants excreting high amounts of indoleacetic acid. Canadian Journal of Microbiology, 29(8), 916-923. https://doi.org/10.1139/m83-147
International Seed Testing Association (1993). Poceedings of International Seed Testing Association. International rules for seed testing. Seed Sci Technol, 21:1-152.
Khamna, S., Yokota, A., & Lumyong, S. (2009). Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production. World Journal of Microbiology and Biotechnology, 25(4), 649-655.
King, E. J. (1932). The colorimetric determination of phosphorus. Biochemical Journal, 26(2), 292-297. https://doi.org/10.1042/bj0260292
Kumar, V., Shahid, M., Srivastava, M., Singh, A., Pandey, S., & Sharma, A. (2014). Enhancing seed germination and vigor of chickpea by using potential and effective strains of Trichoderma species. Virology & Mycology, 3(2), 1-3.
Narayanasamy, P., & Saravana, M. (2009). RAPD analysis of Trichoderma and it’s antagonistic affect with Pseudomonas fluorescens. J. Plant Pathol. Microb., 20(4), 223-233.
Ons, L., Bylemans, D., Thevissen, K., & Cammue, B. (2020). Combining biocontrol agents with chemical fungicides for integrated plant fungal disease control. Microorganisms, 8(12), 1930.
Paredes, J. A., Cazón, L. I., Osella, A., Peralta, V., Alcalde, M., Kearney, M. I., ... & Oddino, C. (2016). Relevamiento regional del carbon del mani y estimaciones de perdidas producidas por la enfermedad. XXXI Jornada Nacional de Mani, 53-54.
Prasad, R., Sagar, B. V., Devi, G. U., Triveni, S., Rao, S. K., & Chari, D. (2017). Isolation and screening of bacterial and fungal isolates for plant growth promoting properties from tomato (Lycopersicon esculentum Mill.). Int. J. Curr. Microbiol. App. Sci, 6(8), 753-761.
Prasad, R. D., Chandrika, K. S. V. P., & Godbole, V. (2020). A novel chitosan biopolymer based Trichoderma delivery system: Storage stability, persistence and bio efficacy against seed and soil borne diseases of oilseed crops. Microbiological Research, 237, 126487.
Rajaput, J., & Rao, M. (2019). In vitro evaluation of antagonist’s agents against seed-borne fungal diseases of tomato (Solanumly copersicum Mill.). Journal of Pharmacognosy and Phytochemistry, 8(6), 574-576.
Rifai, M. A. (1969). A revision of the genus Trichoderma. Mycological Papers, 116, 1-56.
Samuels, G. J., Dodd, S. L., Gams, W., Castlebury, L. A., & Petrini, O. (2002). Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia, 94(1), 146-170. https://doi.org/10.1080/15572536.2003.11833257
Schwyn, B., & Neilands, J. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160(1), 47-56. https://doi.org/10.1 016/0003-2697(87)90612-9
Shahid, D. M. (2013). Molecular characterization of Trichoderma longibrachiatum 21PP isolated from rhizospheric soil based on universal ITS primers. African Journal of Microbiology Research, 7, 4902-4906. https://doi.org/10.5897/AJMR2013.5761
Sharma, K., & Singh, U. (2014). Cultural and morphological characterization of rhizospheric isolates of fungal antagonist Trichoderma. Journal of Applied and Natural Science, 6(2), 451-456.
Siddiquee, S. (2017). Fungal volatile organic compounds: emphasis on their plant growth-promoting. In Volatiles and Food Security (pp. 313-333). Springer, Singapore.
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A Guide to Methods and Aapplications, 18(1), 315-322.
Zhao, L., Wang, F., Zhang, Y., & Zhang, J. (2014). Involvement of Trichoderma asperellum strain T6 in regulating iron acquisition in plants. Journal of Basic Microbiology, 54(S1), S115-S124. https://doi.org/10.1002/jobm.20 1400148
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Ayyandurai, M. ., Akila, R. ., Manonmani, K. ., Theradimani, M. ., & Vellaikumar, S. . (2021). Phytostimulation and growth promotion activities of Trichoderma spp. on groundnut (Arachis hypogaea L.) crop. Journal of Applied and Natural Science, 13(4), 1172–1179. https://doi.org/10.31018/jans.v13i4.2936
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