Modified Ninhydrin reagent for the detection of amino acids on TLC plates
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Abstract
Ninhydrin is the most well known spray reagent for identification of amino acids due to its high sensitivity. But, it produces same purple/violet color with all amino acids, except proline and hydroxy proline. A new spray reagent, para bromobenzoic acid has been introduced here which produces distinguishable colors with the amino acids with moderately high sensitivity (0.01-1.0) ?g. A probable mechanism for such color formation has also been proposed.
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Keywords
Amino acids, Ninhydrin, p-Bromobenzoic acid, Thin-layer chromatography
References
Basak, B. and Laskar, S. (1990). Spray reagent for the detection of amino-acids on thin-layer Plates. Talanta, 37:1105-1106.
Basak, B., Bhattacharya, U.K. and Laskar, S. (1993). Spray reagent for the detection of amino acids on thin layer chromatography plates. Amino Acids, 4:193-196.
Das, D., Sahana, A., Saha, R., Kundu, P. and Laskar, S. (2010). Anthracene-anchored derivatized methionine: A new ligand for detection of amino acids, and estimation of binding constants. Journal of Planar Chromatography, 23:255-259.
Devaux, G. and Mesnard, P. (1971). Mechanism of the color reaction between ascorbic acid and amino acids. Bulletin of the Society of Pharmaceutical Sciences Bordeaux, 110:145-151.
Distler, W. and Fresenius, Z. (1981). 1-Chloro-7-nitrobenzoic- 1, 2, 5 oxadiazolein Spruhreagenszur Fluoreszenz-Detection von Aminosauren auf Dunnschicht-Chromatogrammen. Analytical Chemistry, 309:127-128.
Foster, R. (1969). Organic charge transfer complexes, Academic Press, New York.
Khawas, S. and Laskar, S. (2003a). A reagent for the detection of amino acids on thin layer chromatography plates. Asian Journal of Chemistry, 15:512-514.
Khawas, S. and Laskar, S. (2003b). Two new spray reagents for the detection of amino acids on thin- layer plates. Journal of Planar Chromatography, 16:165-166.
Khawas, S., Panja, D. and Laskar, S. (2004). Identification of amino acids on TLC plates with a new reagent. Journal of Planar Chromatography, 17:314-315.
Laskar, S. and Basak, B. (1988). Detection of amino acids on thin-layer plates. Journal of Chromatography, 436:341-343.
Laskar, S. and Basak, B. (1990). Identification of amino acids with ninhydrin in acid media. Journal of Planar Chromatography, 3:535-536.
Laskar, S., Bhattacharya, U. and Basak, B. (1991). Modified ninhydrin spray reagent for the identification of amino acids on thin-layer chromatography plates. Analyst, 116:625-626.
Laskar, S., Sinhababu, A. and Hazra, K.M. (2001a). A modified spray reagent for the identification of amino acids on thin layer chromatography plates. Journal of Indian Chemical Society, 78:49-50.
Laskar, S., Panja, D. and Sinhababu, A. (2001b). A spray reagent for identification of amino acids on thin-layer chromatography plates. Journal of Planar Chromatography, 14:462-463.
Laskar, S., Sinhababu, A. and Hazra, K.M. (2001c). A modified spray reagent for the detection of amino acids on thin layer chromatography plates. Amino Acids, 21:201-204.
Lorentz, K. and Flatter, B. (1970). Staining of amino acids with benzoquinone in paper chromatography. Analytical Biochemistry, 38:557-559.
Samanta, T.D., Nag, M. and Laskar, S. (2004). A new spray reagent for identification of amino acids on thin-layer chromatography plates. Asian Journal of Chemistry, 16:1947-1949.
Samanta, T.D. and Laskar, S. (2006). New reagent for detection of amino acids on TLC plates. Journal of Planar Chromatography, 19:252-254.
Sahana, A., Das, S., Saha, R., Gupta, M., Laskar, S. and Das, D. (2011). Identification and interaction of amino acids with leucine-anthracene reagent by TLC and spectrophotometry: experimental and theoretical studies. Journal of Chromatographic Science, 49:652-656.
Sinhababu, A., Basak, B. and Laskar, S. (1994). Novel spray reagent for identification of amino acids on thin-layer chromatography plates. Analytical Proceedings Including Analytical Communications, 31:65-66.
Sinhababu, A., Basak, B., Dey, H. and Laskar, S. (2013). Identification of amino acids with modified ninhydrin reagents on thin layer chromatography plates. Journal of Planar Chromatography, 26:26-30.
Stahl, E. (1969). Thin layer chromatography: A laboratory handbook (2nd ed) Springer, New York.
Wolski, T., Golkiewicz, W. and Rompola, A. (1980). 4-N, Ndimethyl aminoazobenzene-4-sulfonyl chloride-a high sensitive reagent for the spectrophotometric and chromatographic analysis of amino acids. Chemical Analysis (Warsaw), 25:583-590.
Basak, B., Bhattacharya, U.K. and Laskar, S. (1993). Spray reagent for the detection of amino acids on thin layer chromatography plates. Amino Acids, 4:193-196.
Das, D., Sahana, A., Saha, R., Kundu, P. and Laskar, S. (2010). Anthracene-anchored derivatized methionine: A new ligand for detection of amino acids, and estimation of binding constants. Journal of Planar Chromatography, 23:255-259.
Devaux, G. and Mesnard, P. (1971). Mechanism of the color reaction between ascorbic acid and amino acids. Bulletin of the Society of Pharmaceutical Sciences Bordeaux, 110:145-151.
Distler, W. and Fresenius, Z. (1981). 1-Chloro-7-nitrobenzoic- 1, 2, 5 oxadiazolein Spruhreagenszur Fluoreszenz-Detection von Aminosauren auf Dunnschicht-Chromatogrammen. Analytical Chemistry, 309:127-128.
Foster, R. (1969). Organic charge transfer complexes, Academic Press, New York.
Khawas, S. and Laskar, S. (2003a). A reagent for the detection of amino acids on thin layer chromatography plates. Asian Journal of Chemistry, 15:512-514.
Khawas, S. and Laskar, S. (2003b). Two new spray reagents for the detection of amino acids on thin- layer plates. Journal of Planar Chromatography, 16:165-166.
Khawas, S., Panja, D. and Laskar, S. (2004). Identification of amino acids on TLC plates with a new reagent. Journal of Planar Chromatography, 17:314-315.
Laskar, S. and Basak, B. (1988). Detection of amino acids on thin-layer plates. Journal of Chromatography, 436:341-343.
Laskar, S. and Basak, B. (1990). Identification of amino acids with ninhydrin in acid media. Journal of Planar Chromatography, 3:535-536.
Laskar, S., Bhattacharya, U. and Basak, B. (1991). Modified ninhydrin spray reagent for the identification of amino acids on thin-layer chromatography plates. Analyst, 116:625-626.
Laskar, S., Sinhababu, A. and Hazra, K.M. (2001a). A modified spray reagent for the identification of amino acids on thin layer chromatography plates. Journal of Indian Chemical Society, 78:49-50.
Laskar, S., Panja, D. and Sinhababu, A. (2001b). A spray reagent for identification of amino acids on thin-layer chromatography plates. Journal of Planar Chromatography, 14:462-463.
Laskar, S., Sinhababu, A. and Hazra, K.M. (2001c). A modified spray reagent for the detection of amino acids on thin layer chromatography plates. Amino Acids, 21:201-204.
Lorentz, K. and Flatter, B. (1970). Staining of amino acids with benzoquinone in paper chromatography. Analytical Biochemistry, 38:557-559.
Samanta, T.D., Nag, M. and Laskar, S. (2004). A new spray reagent for identification of amino acids on thin-layer chromatography plates. Asian Journal of Chemistry, 16:1947-1949.
Samanta, T.D. and Laskar, S. (2006). New reagent for detection of amino acids on TLC plates. Journal of Planar Chromatography, 19:252-254.
Sahana, A., Das, S., Saha, R., Gupta, M., Laskar, S. and Das, D. (2011). Identification and interaction of amino acids with leucine-anthracene reagent by TLC and spectrophotometry: experimental and theoretical studies. Journal of Chromatographic Science, 49:652-656.
Sinhababu, A., Basak, B. and Laskar, S. (1994). Novel spray reagent for identification of amino acids on thin-layer chromatography plates. Analytical Proceedings Including Analytical Communications, 31:65-66.
Sinhababu, A., Basak, B., Dey, H. and Laskar, S. (2013). Identification of amino acids with modified ninhydrin reagents on thin layer chromatography plates. Journal of Planar Chromatography, 26:26-30.
Stahl, E. (1969). Thin layer chromatography: A laboratory handbook (2nd ed) Springer, New York.
Wolski, T., Golkiewicz, W. and Rompola, A. (1980). 4-N, Ndimethyl aminoazobenzene-4-sulfonyl chloride-a high sensitive reagent for the spectrophotometric and chromatographic analysis of amino acids. Chemical Analysis (Warsaw), 25:583-590.
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How to Cite
Modified Ninhydrin reagent for the detection of amino acids on TLC plates. (2013). Journal of Applied and Natural Science, 5(1), 125-127. https://doi.org/10.31018/jans.v5i1.292
