Modified Ninhydrin reagent for the detection of amino acids on TLC plates
Article Main
Abstract
Ninhydrin is the most well known spray reagent for identification of amino acids due to its high sensitivity. But, it produces same purple/violet color with all amino acids, except proline and hydroxy proline. A new spray reagent, para bromobenzoic acid has been introduced here which produces distinguishable colors with the amino acids with moderately high sensitivity (0.01-1.0) ?g. A probable mechanism for such color formation has also been proposed.
Article Details
Article Details
Keywords
Amino acids, Ninhydrin, p-Bromobenzoic acid, Thin-layer chromatography
References
Basak, B. and Laskar, S. (1990). Spray reagent for the detection of amino-acids on thin-layer Plates. Talanta, 37:1105-1106.
Basak, B., Bhattacharya, U.K. and Laskar, S. (1993). Spray reagent for the detection of amino acids on thin layer chromatography plates. Amino Acids, 4:193-196.
Das, D., Sahana, A., Saha, R., Kundu, P. and Laskar, S. (2010). Anthracene-anchored derivatized methionine: A new ligand for detection of amino acids, and estimation of binding constants. Journal of Planar Chromatography, 23:255-259.
Devaux, G. and Mesnard, P. (1971). Mechanism of the color reaction between ascorbic acid and amino acids. Bulletin of the Society of Pharmaceutical Sciences Bordeaux, 110:145-151.
Distler, W. and Fresenius, Z. (1981). 1-Chloro-7-nitrobenzoic- 1, 2, 5 oxadiazolein Spruhreagenszur Fluoreszenz-Detection von Aminosauren auf Dunnschicht-Chromatogrammen. Analytical Chemistry, 309:127-128.
Foster, R. (1969). Organic charge transfer complexes, Academic Press, New York.
Khawas, S. and Laskar, S. (2003a). A reagent for the detection of amino acids on thin layer chromatography plates. Asian Journal of Chemistry, 15:512-514.
Khawas, S. and Laskar, S. (2003b). Two new spray reagents for the detection of amino acids on thin- layer plates. Journal of Planar Chromatography, 16:165-166.
Khawas, S., Panja, D. and Laskar, S. (2004). Identification of amino acids on TLC plates with a new reagent. Journal of Planar Chromatography, 17:314-315.
Laskar, S. and Basak, B. (1988). Detection of amino acids on thin-layer plates. Journal of Chromatography, 436:341-343.
Laskar, S. and Basak, B. (1990). Identification of amino acids with ninhydrin in acid media. Journal of Planar Chromatography, 3:535-536.
Laskar, S., Bhattacharya, U. and Basak, B. (1991). Modified ninhydrin spray reagent for the identification of amino acids on thin-layer chromatography plates. Analyst, 116:625-626.
Laskar, S., Sinhababu, A. and Hazra, K.M. (2001a). A modified spray reagent for the identification of amino acids on thin layer chromatography plates. Journal of Indian Chemical Society, 78:49-50.
Laskar, S., Panja, D. and Sinhababu, A. (2001b). A spray reagent for identification of amino acids on thin-layer chromatography plates. Journal of Planar Chromatography, 14:462-463.
Laskar, S., Sinhababu, A. and Hazra, K.M. (2001c). A modified spray reagent for the detection of amino acids on thin layer chromatography plates. Amino Acids, 21:201-204.
Lorentz, K. and Flatter, B. (1970). Staining of amino acids with benzoquinone in paper chromatography. Analytical Biochemistry, 38:557-559.
Samanta, T.D., Nag, M. and Laskar, S. (2004). A new spray reagent for identification of amino acids on thin-layer chromatography plates. Asian Journal of Chemistry, 16:1947-1949.
Samanta, T.D. and Laskar, S. (2006). New reagent for detection of amino acids on TLC plates. Journal of Planar Chromatography, 19:252-254.
Sahana, A., Das, S., Saha, R., Gupta, M., Laskar, S. and Das, D. (2011). Identification and interaction of amino acids with leucine-anthracene reagent by TLC and spectrophotometry: experimental and theoretical studies. Journal of Chromatographic Science, 49:652-656.
Sinhababu, A., Basak, B. and Laskar, S. (1994). Novel spray reagent for identification of amino acids on thin-layer chromatography plates. Analytical Proceedings Including Analytical Communications, 31:65-66.
Sinhababu, A., Basak, B., Dey, H. and Laskar, S. (2013). Identification of amino acids with modified ninhydrin reagents on thin layer chromatography plates. Journal of Planar Chromatography, 26:26-30.
Stahl, E. (1969). Thin layer chromatography: A laboratory handbook (2nd ed) Springer, New York.
Wolski, T., Golkiewicz, W. and Rompola, A. (1980). 4-N, Ndimethyl aminoazobenzene-4-sulfonyl chloride-a high sensitive reagent for the spectrophotometric and chromatographic analysis of amino acids. Chemical Analysis (Warsaw), 25:583-590.
Basak, B., Bhattacharya, U.K. and Laskar, S. (1993). Spray reagent for the detection of amino acids on thin layer chromatography plates. Amino Acids, 4:193-196.
Das, D., Sahana, A., Saha, R., Kundu, P. and Laskar, S. (2010). Anthracene-anchored derivatized methionine: A new ligand for detection of amino acids, and estimation of binding constants. Journal of Planar Chromatography, 23:255-259.
Devaux, G. and Mesnard, P. (1971). Mechanism of the color reaction between ascorbic acid and amino acids. Bulletin of the Society of Pharmaceutical Sciences Bordeaux, 110:145-151.
Distler, W. and Fresenius, Z. (1981). 1-Chloro-7-nitrobenzoic- 1, 2, 5 oxadiazolein Spruhreagenszur Fluoreszenz-Detection von Aminosauren auf Dunnschicht-Chromatogrammen. Analytical Chemistry, 309:127-128.
Foster, R. (1969). Organic charge transfer complexes, Academic Press, New York.
Khawas, S. and Laskar, S. (2003a). A reagent for the detection of amino acids on thin layer chromatography plates. Asian Journal of Chemistry, 15:512-514.
Khawas, S. and Laskar, S. (2003b). Two new spray reagents for the detection of amino acids on thin- layer plates. Journal of Planar Chromatography, 16:165-166.
Khawas, S., Panja, D. and Laskar, S. (2004). Identification of amino acids on TLC plates with a new reagent. Journal of Planar Chromatography, 17:314-315.
Laskar, S. and Basak, B. (1988). Detection of amino acids on thin-layer plates. Journal of Chromatography, 436:341-343.
Laskar, S. and Basak, B. (1990). Identification of amino acids with ninhydrin in acid media. Journal of Planar Chromatography, 3:535-536.
Laskar, S., Bhattacharya, U. and Basak, B. (1991). Modified ninhydrin spray reagent for the identification of amino acids on thin-layer chromatography plates. Analyst, 116:625-626.
Laskar, S., Sinhababu, A. and Hazra, K.M. (2001a). A modified spray reagent for the identification of amino acids on thin layer chromatography plates. Journal of Indian Chemical Society, 78:49-50.
Laskar, S., Panja, D. and Sinhababu, A. (2001b). A spray reagent for identification of amino acids on thin-layer chromatography plates. Journal of Planar Chromatography, 14:462-463.
Laskar, S., Sinhababu, A. and Hazra, K.M. (2001c). A modified spray reagent for the detection of amino acids on thin layer chromatography plates. Amino Acids, 21:201-204.
Lorentz, K. and Flatter, B. (1970). Staining of amino acids with benzoquinone in paper chromatography. Analytical Biochemistry, 38:557-559.
Samanta, T.D., Nag, M. and Laskar, S. (2004). A new spray reagent for identification of amino acids on thin-layer chromatography plates. Asian Journal of Chemistry, 16:1947-1949.
Samanta, T.D. and Laskar, S. (2006). New reagent for detection of amino acids on TLC plates. Journal of Planar Chromatography, 19:252-254.
Sahana, A., Das, S., Saha, R., Gupta, M., Laskar, S. and Das, D. (2011). Identification and interaction of amino acids with leucine-anthracene reagent by TLC and spectrophotometry: experimental and theoretical studies. Journal of Chromatographic Science, 49:652-656.
Sinhababu, A., Basak, B. and Laskar, S. (1994). Novel spray reagent for identification of amino acids on thin-layer chromatography plates. Analytical Proceedings Including Analytical Communications, 31:65-66.
Sinhababu, A., Basak, B., Dey, H. and Laskar, S. (2013). Identification of amino acids with modified ninhydrin reagents on thin layer chromatography plates. Journal of Planar Chromatography, 26:26-30.
Stahl, E. (1969). Thin layer chromatography: A laboratory handbook (2nd ed) Springer, New York.
Wolski, T., Golkiewicz, W. and Rompola, A. (1980). 4-N, Ndimethyl aminoazobenzene-4-sulfonyl chloride-a high sensitive reagent for the spectrophotometric and chromatographic analysis of amino acids. Chemical Analysis (Warsaw), 25:583-590.
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Modified Ninhydrin reagent for the detection of amino acids on TLC plates. (2013). Journal of Applied and Natural Science, 5(1), 125-127. https://doi.org/10.31018/jans.v5i1.292
