In vitro anti-inflammatory, anti-diabetic and antioxidant potential of Cissus quadrangularis along with its orexigenic activity in Drosophila melanogaster

Abstract

Plants with diverse pharmacological activities are actively being explored for human health. Cissus quadrangularis (L) has been reported to possess numerous phytochemicals and is used to relieve various disorders. This article aims at providing evidence of the diverse pharmacological activities in terms of orexigenic, anti-inflammatory, anti-diabetic and antioxidant activities of C. quadrangularis for further application in clinical development. The results revealed that inhibition of hemolysis was within the range of 8-9-25.6% at concentrations of 12.5-200 µg/ml. Methanol extract of C. quadrangularis stems exhibited porcine pancreatic ?-amylase (PPA) inhibition (p?0.05) at concentrations of 0.25 and 0.30 mg/ml. The glucose adsorption capacity of the C. quadrangularis was observed to be inversely proportional to the molar concentration of glucose. The higher food intake by Drosophila in food medium with plant extract is presumably related to orexigenic property of C. quadrangularis. Protease activity of C. quadrangularis stem extract revealed total activity 975 U/ml and specific activity as 3768 U/mg. The absorbance of C. quadrangularis in reducing power assay were between 0.91and 1.85. Highest total antioxidant activity of 67.2 µg TE/g was observed and the hydroxyl radicals scavenging activity was observed in a dose dependent manner. The results provide supporting data that C. quadrangularis may contain active compounds useful in treating anti-inflammatory, anti-diabetic disorders.