Culture and physiological variability in Rhizoctonia solani, responsible for foliar and lesions on aerial part of soybean

Article Sidebar

PDF
Published Jun 1, 2013
DOI: https://doi.org/10.31018/jans.v5i1.279

Article Main

Lalan Sharma
Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar- 263145 (UK), India
Sanjay Goswami
Department of Plant Pathology, Punjab Agriculture University, Ludhiana- 141 004, India
D. T. Nagrale
Department of Plant Pathology and Agricultural Microbiology, Mahatma Phoole Agricultural University, Rahuri- Rahuri-413722, India

Abstract

Foliar blight of soybean is one of the major fungal diseases. Rhizoctonia solani isolated from soybean growing in tarai regions of Uttarakhand. Six isolates of R. solani has been characterized on the basis of cultural and physiological nature such as colony diameter, growth, colour and sclerotia formation were recorded. Potato Dextrose Agar (PDA) was found best for growth and development. Two isolates (Lakhimpur and Pantnagar) covered the whole plates (90 mm) in 48 hrs. However, maximum number of sclerotia and weight was recorded on Czapek Dox agar medium. Overall radial growth supporting is recorded Corn Meal Agar Medium. Varied range of temperatures i.e. 10, 15, 25, 30, 35 and 400C was tested and found better growth of different isolates of R. solani at 10 - 400C, with an optimum growth temperature at 300C. Isolates were grown on five broth media (Asthana & Hawkers, Potato Dextrose Agar, Czapek’s Dox Agar, Corn Meal Agar and Richards Agar) for fresh, dry weight and oat meal broth culture filtrates of all isolates was used in phytotoxic effects. It recorded that maximum fresh and dry weight was observed on corn meal agar medium. The maximum reduction in radical and plumule length of germinating seeds were recorded in Haldichaur isolate.

Article Details

Article Details

Keywords

Cultural, Foliar blight, Physiological, Phytotoxic, Rhizoctonia solani and Soybean

References
Anwar, S.A., Apas, S.F., Gill, M.M., Rauf, C, A., Mahmood, S. and Bhutta, A.R. (1995). Seed borne fungi of soybean and their effect on seed germination. Pakistan J. Phytopath. 7: 184-190.
Atkins, J.G. and Lewis, W.D. (1954). Rhizoctonia aerial blight of soybean in Louisiana. Phytopathology. 44: 1.
Burpee, L.L., Sander, P.L. and Sherwood, R.T. (1980). Anastomosis group among isolates of Ceratobasidium cornigerum (Bourd) Roger and related fungi. Mycologia 72: 689-701.
Dubey, S.C. and Dwivedi, R.P. (1992). Evaluation of fungicides against the web blight disease of groundnut. Agric. Sci. Digest., 12: 90-92.
Fenille, R. C., Souza, N. L. D. and Kuramae, E. E. (2002). Characterization of Rhizoctonia solani associated with soybean in Brazil. European J. Pl. Pathol., 108 : 783-792.
Grosch, R. and Kofoet, A. (2003). Influence of temperature, pH and inoculum density on bottom rot on letuce caused by Rhizoctonia solani. Zeitschrift-fur-Pflanzenkrankheiten-und- Pflanzenschutzt, 110(4): 366-378.
Jain, R. K. and Thapliyal, P. N. (1980). Toxic metabolites from Rhizoctonia solani Kuhn : Production and possible role in pathogenesis. Indian J. Expt. Biol., 18: 316-318.
Lim, T. K., N.G C.C. and Chin, C.L. (1987). Etiology and control of durian foliar blight and die back caused by Rhizoctonia solani. Ann. Appl. Biol., 11: 301-307.
Mckinney, H. H. (1923). Influence of soil temperature and moisture on infection wheat seedling by Halminthosporium sativum. J. Agric. Res. 26: 195 – 210.
Munsell (1954) Munsell’s Soil Colour Chart. 1954. Munsell colour Co. Inc. Baltimore, Maryaland, U.S.A.
Rangaswami, G. and Mahadevan, A. (Eds) (2004). Disease of crop plants in India. Prentice-Hall of India Private Limited Publisher, New Delhi, India. pp. 507.
Sherwood, R. T. and Lindberg CG. (1962). Production of a phytotoxin by Rhizoctonia solani. Phytopathology, 52 : 586-587.
Singh, R., Shukla, T.N., Dwivedi, R.P., Shukla, H.P. and Singh, P.N. (1974). Studies on the soybean blight caused by Rhizoctonia solani. Indian J. Mycol. Pl. Path., 4: 101-103.
Sinha, B.B.P., and Ghufran, S.M. (1988). Physiological studies on five isolates of sheath blight of rice caused by R. solani Kuhn. Journal of Research-Rajendra Agricultural University. 6: 61-67.
Sunder, S., Singh, R. and Dodan, D.S. (2003). Standardization of inoculation methods and management of sheath blight of rice. Indian J Plant Pathol., 21: 92-96.
Tiwari, A. and Khare, M.N. (2002). Conditions inducing imperfect and perfect stages in Rhizoctonia solani causing diseases of mungbean. J. Mycol. Pl. Path., 32(2): 176-180.
Verma, H.S. (1973). Rhizoctonia aerial blight of soybean symptomotology, etiology and chemical control. M.Sc. Thesis, G.B.P.U.A.&T., Pantnagar. pp. 58.
Wrather, J. A., Stienstra, W. C., and Koenning, S. R. (2001). Soybean disease loss estimates for the United States from 1996 to 1998. Can. J. Plant Pathol. 23:122-131.
Issue
Vol 5 No 1 (2013)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Culture and physiological variability in Rhizoctonia solani, responsible for foliar and lesions on aerial part of soybean. (2013). Journal of Applied and Natural Science, 5(1), 41-46. https://doi.org/10.31018/jans.v5i1.279