Estimation of ferulic acid from selected plant materials by Spectrophotometry and High performance liquid chromatography
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Abstract
Ferulic acid is an abundant phytophenolic compound present in plant cell wall. Ferulic acid possess anticancer, antioxidant, and anti-aging properties. A simple, sensitive and reproducible spectrophotometric method has been developed for quantitative estimation of ferulic acid from selected plant materials such as rice bran, wheat bran and bamboo shoot. The blue coloured chromogen obtained after the reaction was measured at wavelength of 718 nm for ferulic acid against the blank reagent. The chromogen obeyed linearity over the range of 1?g/ml - 8?g/ml. An HPLC method was also developed for the estimation of ferulic acid from selected plant materials.
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Article Details
Bamboo shoot, Ferulic acid, Rice bran, Spectrophotometry, Wheat bran
2. Buranov, AU and Mazza, G (2009). Extraction and purification of ferulic acid from flax shives, wheat and corn bran by alkaline hydrolysis and pressurised solvents. Food Chem. 115, 1542–1548.
3. Kuenzig, J Chau, E Norkus, H Holowaschenko, H Newmark, W Mergens and A H Conney (1984). Caffeic and ferulic acid as blockers of nitrosamine formation. Carcinogenesis. 5, 309-313.
4. Roginsky, V., Lissi, E.A (2005). Review of methods to determine chain-breaking antioxidant activity in food. Food Chem. 92, 235-254.
5. Dejian H., Boxin O., Ronald LP (2005). The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53, 1841 – 1846.
6. Ali H., Dixit S (2012). Identification of Chemical Constituent of Combine Sample of Some Medicinal Plants for Antioxidant Activity. Asian J. Plant Sci. Res. 2(3), 269-273.
7. Choo WS., Yong WK (2011). Antioxidant properties of two species of Hylocereus fruits. Adv. Appl. Sci. Res. 2(3), 418-425.
8. Yousuf M., Aslam K., Wani BA., Aslam N., Dar NA., Nawchoo IA (2012). In vitro antibacterial activity and phytochemical studies of methanolic extract of leaves of Hypericum perforatum L. growing wild in Kashmir Himalaya. Asian J. Plant Sci. Res. 2(4), 414-420.
9. Kumar, N and Pruthi, V (2014). Potential applications of ferulic acid from natural sources. Biotechnology Reports. 4: 86–93.
10. Yousuf M., Aslam K., Wani BA., Aslam N., Dar NA., Nawchoo IA (2012). In vitro antibacterial activity and phytochemical studies of methanolic extract of leaves of Hypericum perforatum L. growing wild in Kashmir Himalaya. Asian J. Plant Sci. Res. 2(4), 414-420.
11. Ovando C., Hernandez D., Hernandez E, et al. (2009). Chemical studies of anthocyanins: a review. Food Chem. 113, 859–871.
12. Lee Y., Yuk D, Lee J., et al. (2009). Epigallocatechin-3-gallate prevents lipopolysaccharide-induced elevation of ?-amyloid generation and memory deficiency. Brain Res. 1250, 164–174.
13. Divakaran, SA., Hema, PS., Nair, MS., Nair, CKK (2013). Antioxidant capacity and radioprotective properties of the flavonoids galangin and kaempferide isolated from Alpinia galanga L. (Zingiberaceae) against radiation induced cellular DNA damage. Int. J. Radiat. Res.11(2): 81-89.
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