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Gunjan Thakur K. S. Kapoor Ashwani Tapwal

Abstract

Rhizopogon himalayensis (Castellano, S.L. Miller, Singh & Lakhanpal) A.B. Mujic & M.E. Sm., comb. nov. -an edible truffle-like fungus- normally exists in a symbiotic association with Cedrus deodara (Roxb.) Loud. Because of this important association and the ecological significance as per the available literature attached to this fungus, the present study was undertaken with a specific objective to test the mycorrhizal viability of this mycobiont with this important tree species of Himalaya -the theme tree of Himachal Pradesh. An attempt to investigate and record morphological and anatomical characteristics and variations in this mycorrhizal association was also made in nursery conditions using pure culture (wheat grain spawn) of R. himalayensis. The seedlings grown and inoculated in the nursery were harvested after six months for further examination, including the microscopic details. The study and subsequent analysis revealed that that mycobiont had invaded most of the feeder roots, imparting a typical swollen appearance to the mycorrhizal root tips. These root tips were light ochre with moderately thick plectenchymatic fungal mantle with occasional clamp connections on the inner layer of the fungal mantle. The root surface, as seen, was found smooth and frequently covered with a loose aggregation of inter-woven hyphae that uniformly pervaded the epidermis extending into the inter-cortical spaces of outer cortical cells and formed a characteristic Hartig net. Thus, results obtained in fact for the first time, presented an in-depth analysis of the morphological and anatomical characteristics of R. himalayensis and C. deodara association. 

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Keywords

Cedrus deodara, Ectomycorrhiza, Hyphae, Morpho-anatomy, Rhizopogon himalayensis

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Thakur, G., Kapoor , K. S., & Tapwal, A. . (2021). Morpho-anatomical characterization of Rhizopogon himalayensis - Cedrus deodara mycorrhiza. Journal of Applied and Natural Science, 13(2), 668 - 676. https://doi.org/10.31018/jans.v13i2.2674
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