Article Main

Monika Patel Sanjeev Kumar

Abstract

Pigeonpea is one of the important pulse crop of Madhya Pradesh, a State of India. The plant gets infected by the pathogen Fusarium udum causing wilt disease, which is one of the major constraints in the production and productivity of pigeonpea. The present study aimed to carry out in vitro condition to assess the possible use of biocontrol consortia in field conditions. Six Trichoderma consortia  viz., T1- T. viride  + T. harzianum (JC-I), T2- T. viride + T. virens (JC-2), T3:- T. harzianum +T. virens (JC-3), T4- T. hamatum  +T. viride (JC-4), T5- T. hamatum  + T. harzianum (JC-5), T6- T. hamatum +    T. virens (JC-6)   and T0-  Control were evaluated for their antagonistic activity against F. udumunder in vitro conditions. The consortia of T4- T. hamatum  +T. viride (JC-4)   was found most effective(58.82 %) in inhibiting the  radial growth of Fusarium udum. The volatile compound from consortium of T5-T. hamatum  + T. harzianum (JC-5) exhibited maximum growth inhibition (81.84%) and sporulation  of Fusarium udum followed by T1-T. viride  + T. harzianum (JC-I) (55.49% inhibition) . The culture filtrate of  consortia of T4- T. hamatum  +T. viride (JC-4) showed 100% inhibition of test pathogen followed by T5-T. hamatum  + T. harzianum (JC-5) (82.89%)  at 5 %   concentration. It was also observed that with an increase in the concentration of culture filtrates of all the Trichoderma species, the radial mycelial growth of the test pathogen was proportionally decreased. The Trichodema consortium viz., T4- T. hamatum  +T. viride (JC-4)  may be tried in the field to manage wilt of pigeonpea because they worked synergistically and gave the high impact of their use.

Article Details

Article Details

Keywords

Consortia, Fusarium udum, Pigeonpea, Trichoderma, Wilt

References
Amirthalingam, V., Tewari, A.K., Sharma, M., Sharma R., & Kumar, J. (2020). Evaluation of bioagents for their compatibility in the development of consortium for enhanced efficiency. J. Bio. Cont., 34(2), 164-167, 2020, DOI: 10.18311/jbc/2020/23179
Biswas, K. & Ghosh, P.(2016), Recent Advancements and Biological Management of Fusarium udum: A causative agent of Pigeon pea wilt. J. App. & Natu. Sci., 5(3), 57-72 (20
Chaudhary, B., Kumar,S & Kushwaha, S. K. (2017). Bio-efficacy of Trichoderma species against Pigeonpea wilt pathogen. J. App. & Nat. Sci., 9(4), 2327-2331. https://doi.org/10.31018/jans.v9i4.1531
Dennis, C. & Webster, J. (1971a). Antagonistic properties of species groups of Trichoderma II. Production of volatile antibiotics. Trans. Brit. Mycol. Soci., 57, 41-48.
Dennis, C. and Webster, J. (1971b). Antagonistic properties of species groups of TrichodermaI. Production of non volatile antibiotics. Trans. Brit. Mycol. Soci., 57, 25-39.
Izquierdo-García, L. F., González-Almario, A., Cotes, A. M.,& Moreno-Velandia, C. A. (2020). Trichoderma virens Gl006 and Bacillus velezensis Bs006: a compatible interaction controlling Fusarium wilt of cape gooseberry. Sci. Rep., 10,6857. doi: 10.1038/s41598-020-63689-y
Gams, W & Bisset, J. (1998). Morphology and identification of Trichoderma In: Harman G. E. and Kubicek C. P. (eds). Trichoderma & Gliocladium. Vol 1 Taylor and Francis, London.
Harman, G.E. (2011) Multifunctional fungal plant symbionts: new tools to enhance plant growth and productivity. New Phyto. Comm, For. (3), 647-649
Kumar, K.H.& Jagadeesh, K.S. (2016). Microbial consortia-mediated plant defense against phytopathogens and growth benefits. Sou. Ind. J. Biol. Sci., 2(4), 395-403
Kumar, S. (2013). Trichoderma; A biological weapon for managing plant diseases and promoting sustainability. Int. J. Agric. Sci. Vet. Med., 1, 1-16.
Kumar, S., Thakur, M & Rani, A. (2014). Trichoderma: Mass production, formulation, quality control, delivery and its scope in commercialization in India for the management of plant diseases. Afr. J. Agric. Res., 9, 3838-3852.
Kumar, S., Upadhyay, J.P. and Rani, A. (2009). Evaluation of Trichoderma species against Fusarium udum Butler causing wilt of Pigeon pea. J. Biol. Con., 23: 329-332.
Kushwaha, S. K., Kumar, S & Chaudhary, B. (2018). Efficacy of Trichoderma against Sclerotium rolfsii causing collar rot disease of lentil under in vitro conditions. J. App. & Nat. Sci., 10(1), 307-312. https://doi.org/10.31 018/jans.v10i1.1622
Morton,D.T& Straube, N.H. (1955). Antagonistic and stimulatory effects of microorganisms Sclerotium rolfsii. Phytopatho., 45: 419-420.
Nene, Y.L., Kannaiyan, J. and Reddy, M.V. (1981). Pigeonpea diseases: resistance screening techniques. Infor. Bulletin No.9. ICRISAT, India.
Nikam, P.S., Jagtap, G.P & Sontakke, P.L.(2007.) Management of chickpea wilt caused by Fusarium oxysporium f. sp. ciceri. Afr. J. Agri. Res., 2(12), pp.692-697.
Patole, S.P., Dhore, S.B., Pradhan, R.S. & Shankara, K. (2017). In vitro evaluation of Trichoderma viride and Trichoderma harzianum against Fusarium wilt of Chickpea, Int. J. Pure App. Biosci., 5(5): 460-464 doi: http://dx.doi.org/10.18782/2320-7051.5508
Singh , A .I., Formm, G.K., Jha, P. Venkatesh, H., Tewari, R., Padaria, U., Egger .(2020). Understanding pigeonpea (Cajanus cajan) production conditions, stakeholders preferences for varietal traits and their implications for breeding programmes in India. bioRxiv 2020.0 6.08.139832; doi: https://doi.org/10.1101/2020.06.08.13 983
Singh, B.N., Singh, A., Singh, S.P. & Singh, H.B. (2011) Trichoderma harzianum-mediated reprogramming of oxidative stress response in root apoplast of sunflower enhances defence against Rhizoctonia solani. Euro. J. Pl. Patho. 131(1): 121-134.
Thakkar, A. & Saraf, M. (2015). Development of microbial consortia as a biocontrol agent for effective managementof fungal diseases in Glycine max L. Arch Phytopathol. Plant Prot. 48: 459-474.
Sharma, M., Rathore, A., Mangala, U. N., Ghosh, R., Sharma, S. & Upadhyay H. D. (2012). New sources of resistance to Fusarium wilt and sterility mosaic disease in a mini-core collection of pigeonpea germplasm. Eur. J. Plant Pathol. 133, 707–714. 10.1007/s10658-012-9949-9
Vincent, J.M. (1947). Distortion of fungal hyphae in presence of certain inhibitors. Nature, 154:8
Wu, Y., Zhou, J., Li, C., & Ma, Y. (2019). Antifungal and plant growth promotion activity of volatile organic compounds produced by Bacillus amyloliquefaciens. Microbiologyopen, 8(8),e0081310.1002/mbo3.813 
Section
Research Articles

How to Cite

The Evaluation of Trichoderma consortia against Fusarium udum causing wilt of Pigeonpea. (2021). Journal of Applied and Natural Science, 13(2), 537-543. https://doi.org/10.31018/jans.v13i2.2656