Bhumika Chauhan Anshu Chaudhary Anju Tyagi Bindu Sharma Hridaya S Singh


Clinostomum is a fish-borne pathogen,digenetic trematode with worldwide distribution. Despite its zoonootic significance, the molecules involved in the host-parasite interaction remains unknown. The present study deals with the proteome profile of the rDNA of Clinostomum piscidium using in silico workflow.The physicochemical properties, mass spectrometry ,atomic composition,estimated half-life in different hosts ,Grand average of hydropathy (GRAVY),extinction coefficient and instability index of the rDNA was studied.We identified hypothetical proteins(polar in nature) that showed similarities with proteins of Trichellina pseudospiralis and Melampsora laricipopulina.The functionality of these proteins revealed their role in intracellular signalling as a substrate for O - linked N - acetylglucosaminetransferases and death transcription factor(DIDO-1). This is the first report of our findings that aims to provide a better understanding of the mechanism by which this digenetic trematode adapts to extreme environments. It is expected that this study will lead to new insights into drug designing strategies and disease control.


Download data is not yet available.


Metrics Loading ...




Clinostomum piscidium, Colisa fasciatus, GRAVY, rDNA, In silico Mass spectrometry, Proteomics

Apodaca,Gerard.(2017). Role of Polarity Proteins in the Generation and Organization of Apical Surface Protrusions. Journal of Cold Spring Harbor Perspective in Biology,13(3),46-57.
Bloch M.E.,& Schneider J.G. (1801). Systema Ichthyologiae iconibus CX illustratum. Post obitum auctoris opus inchoatum absolvit, correxit, interpolavit Jo. Gottlob Schneider, Saxo. Berolini. Sumtibus Austoris Impressum et Bibliopolio Sanderiano Commissum. Systema Ichthyol., 10(1),12-20
Gasteiger E., Gattiker A., Hoogland C., Ivanyi I., Appel R.D & Bairoch A. (2009).ExPASy. the proteomics server for in-depth protein knowledge and analysis
Journal of Nucleic Acids Research, 31,3784-3788.
Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., Bairoch A & John M. Walker(2005. ). Protein Identification and Analysis Tools on the ExPASy Server.In: Walker, J.M.(Ed.). The Proteomics Protocols Handbook, Totowa Humana Press , 571-607.http://archiveouverte.unige.ch/unige:37793
Hara,H., Miyauchi,Y; Tahara,S & Yamashita,H.(2014).Human laryngitis caused by Clinostomum complanatum Nagoya. Journal.Medical Science,76,181-185
Menezes,Fraga Alana; Junior,Cavalcanti; Martis,Semen& Filho,Moraes.(2019). In Silico Characterization of Meloidogyne Genus Nematode Cellulose Binding Proteins.Journal of Brazilian Archives of Biology and Technology, 62,67-75
Park, C.W; Kim, J.S; Joo, H.S & Kim,J. (2009). A human case of Clinostomum complanatum infection in Korea. Korean J. Parasitol., 47, 401–404
Rojas,M.Ana., Pulido,L.Luis., Futterer, Agnes., Weley,van Karel., Martinez, Carlos & Valencia Alfonso (2005).Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role. Federation of European Biochemical Societies,27,3505-3511
Schmidt,G. D. & Roberts,L. S.(2000).Foundations of Parasitology .Boston: McGraw-Hill .
Shareef,Ahammed P.A.& Abidi,S. M. A.(2012). Incidence and histopathology of encysted progenetic metacercaria of Clinostomum complanatum (Digenea: Clinostomidae) in Channa punctatus and its development in experimental host. Tropical Biomedicine,2(6),421-426
Southwell,T & Prashad,B.(1918). Notes from the Bengal fisheries laboratory 5. Parasites of Indian fishes, with a note on carcinoma in the climbing perch. Rec. Ind. Mus.,15, 341-355
Tiewchaloern, S, Udomkijdecha, S,Suvouttho, S, Chunchamsri, K & Waikagul, J. (1999). Clinostomum trematode from human eye. Southeast Asian J. Trop. Med. Public Health, 30, 382–384.
Zimik, Philayung., Sharma, Sunil & Roy, Bishnupada (2019). Characterization of Clinostomum metacercariae using microscopic and molecular approaches. Annals of Parasitology, 65, 87-97.
Citation Format
How to Cite
Chauhan, B. ., Chaudhary, A. ., Tyagi, A. ., Sharma, B. ., & Singh, H. S. . (2021). Proteomic insights into the biology of Clinostomum piscidium from a fish, Colisa fasciatus in India using computational tools. Journal of Applied and Natural Science, 13(2), 455 - 462. https://doi.org/10.31018/jans.v13i2.2596
More Citation Formats:
Research Articles

Most read articles by the same author(s)