Histopathological alterations in testicular tissue of male rats exposed to arsenic
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Abstract
The present study was designed to investigate the adverse effect of arsenic on testicular tissue of Swiss albino male rats. Sodium arsenite was administered to adult male rats by gavage at the doses 1, 2 and 3 mg/kg body weight for 30 days. After the treatment, the testis were processed for histopathological observations. Sodium arsenite caused remarkable reduction in testicular weight (P<0.05), while the body weight of experimental animals were reduced but not significantly (P<0.05). Histological evaluation revealed dose-dependent, gradual destruction in histoarchitecture of testicular tissue. Sodium arsenite exposure caused complete arrest of spermatogenesis with disfigured seminiferous tubules in the testes .The lumens of the tubules were devoid of spermatids and were in places filled with cellular debris. The germinal epithelium was distorted. At places interstitial odema was also evident. Sertoli and Leydig cells were damaged. Along with structural alterations, fertility rate in experimental animals was significantly decreased at higher doses i.e. 2 and 3 mg/kg, as 100% infertility was observed. After withdrawal of the treatment over a period of 30 days, recovery was observed in low dose groups as few female rats became pregnant. The study concluded that exposure of arsenic causes testicular toxicity in male albino rat.
Article Details
Article Details
Arsenic, Fertility test, Histopathology, Reversibility test, Swiss albino rats, Testicular tissue
Abernathy, C.O., Liu, Y.P., Longfellow, D., Aposhian, H.V., Beck, B.,Fowler, B., Goyer, R., Menzer, R., Rossman, T., Thompson, C. and Waalkes, M. (1999). Arsenic: Health effects, mechanism of actions and research issue. Environ Health Perspect, 107: 593- 597.
Chatterjee, A., Das, D., Mandal, B.K., Samanta, G. and Banerjee, P. (1995).The biggest arsenic calamity in the world. Analyst, 120: 643 - 650.
Centeno, J.A., Mullick, F.G., Martine, Z.L., Page, N. P., Gibb, H. and Longfellow, D. (2002). Pathology related to chronic arsenic exposure. Environ. Health Perspect, 110 (5): 883 - 886.
Dorfman, R.I.; Forcheilli,E. and Gut, M. ( 1963). Androgen biosynthesis and related studies. Recent Prog. Horm. Res., 19 : 251 – 273.
Golub, M.S., Macintosh, M.S. and Baumrind, N.(1998). Developmental and reproductive toxicity of inorganic arsenic: animal studies and human concerns. J Toxicol Environ Health B Crit Rev., 1: 199–241.
IARC (1980). Monographs on the evaluation of carcinogenic risk of chemicals to humans (International Agency for Research on Cancer). In: some metals and metallic compounds. Lyon: IARC Press.Vol. 23 (pp 39–141).
IARC (2004). Monographs on the evaluation of carcinogenic risk of chemicals to humans (International Agency for Research on Cancer). In: Some Drinking Water Disinfectants and Contaminants, including Arsenic. Lyon: IARC Press.Vol. 84 pp 269-477
Jana, K., Jana, S. and Samanta, P.K. (2006). Effects of chronic exposure to sodium arsenite on hypothalamo-pituitarytesticular activities in adult rats: possible an estrogenic mode of action. Reprod Biol Endocrinol, 4 (9): 1- 13.
Liu, S.X., Athar, M., Lippai, I., Waldren, C. and Hei, T.K. (2001). Induction of oxyradicals by arsenic: implication for mechanism of genotoxicity. Proc Natl Acad Sci USA, 98:1643-1648.
Mehranjani, M.S. and Hemadi, M. (2007). The effects of sodium arsenite on the testis structure and sex – hormones in vasectomised rats. Iranian Journal of Reproductive Medicine, 5 (3): 127 – 133.
Nelson, W.O. and Patanelli, D.J. (1965). Chemical control of spermatogenesis. In: Agents Affecting Fertility. (Eds. Austin, C.R. and Perry, J.S.), Little Brown, Boston. Mass. U.S.A. 78
NIH (1985): Guide for the care and use of laboratory animals published by National institute of Health Publication USA, NIH 85-23.
NRC (National Research Council). (1999). Arsenic in the drinking water. Washington, DC: National Academy Press. pp. 1 – 310.
Omura, M., Yamazaki, K., Tanaka, A., Hirata, M., Makita, Y. and Inoue, N. (2000). Changes in the testicular damage caused by Indium Arsenide and Indium Phosphide in hamesters during two years after intratracheal instillations. J. Occup Health , 42 : 196 – 204.
Pershagen, G. (1983). The epidemiology of human arsenic exposure. In : Biological and Environmental effects of arsenic, B.A Flower, (Ed), Elsevier, Amsterdam. Pp 193 – 233.
Pant, N., Murthy, R.C. and Srivastava, S.P. (2004). Male reproductive toxicity of sodium arsenite in mice. Hum Exp Toxicol , 23(8) : 399 – 403.
Paul, H.E., Paul, M.P., Kopko, F., Bender, R.C. and Everett, G. (1953). Carbohydrate metabolism- Studies on the testis of rat for certain nitrofurans. Endocrinology, 53 : 585-588.
Sarkar, M., Biswas, N.M. and Ghosh, D. (1991). Effect of sodium arsenite on testicular Ä5, 3â-HSD and 17â-HSD activies in albino rats : Dose and duration dependent responses. Med Sci Res, 19: 789 – 790.
Sarkar, M., Chowdhury, R.G., Chattopadhayay A. and Biswas, N.M. (2003). Effect of sodium arsenite on spermatogenesis, plasma gonadotrophins and testosterone in rats. Asian J Androl, 5: 27 – 31.
Sarkar, S., Hazra, J., Upadhyay, S.N., Singh, R.K. and Chowdhury, A.R. (2008). Arsenic induced toxicity on testicular tissue of mice. Indian J. Physiol Pharmacol, 52(1): 84 – 90.
Sharpe, R.M., Maddocks, S., Kerr, J.B. (1990). Cell-cell interactions in the control of spermatogenesis as studied using Leydig cell destruction and testosterone replacement. American Journal of Anatomy, 188: 3-20.
Steinberger, E. and Duckett, G.E. (1965). Effect of estrogen and testosterone in the initiation and maintenance of spermatogenesis in the rats. Endocrinology, 76: 1184 - 1189.
Shukla, J.P. and Pandey, K. (1984). Impaired spermatogenesis in arsenic treated freshwater fish, Colisa fasciatus.(Bl. and Sch.). Toxicology Letters, 21(2): 191 – 195.
Tabacova, S., Hunter, E.S. and Balabaeva, L. (1992). Potential role of oxidative damage in developmental toxicity of arsenic. In Arsenic : Exposure and Health effects, ( C.O. Abernathy, R.L. ; Calderon and W.R. Chappell, Eds) pp 135 – 144. Chapman and Hall, London.
Tchounwou, P.B., Patlolla, A.K. and Centeno, J.A.( 2003). Carcinogenic and systemic health effects associated with arsenic exposure- A critical review. Toxicol Pathol, 31(6): 575 - 588.
Wang, A., Holladay, S.D., Wolf, D.C., Ahmed, S.A. and Robertson, J.L.(2006). Reproductive and developmental toxicity of arsenic in rodents: a review. International Journal of Toxicology, 25 (5): 319 – 331.
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