Biochemical basis of systemic acquired resistance induced by different Systemic Acquired Resistance (SAR) elicitors in Brassica cultivars challenge inoculated with downy mildew pathogen
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Abstract
In India, Brassica is attacked by many bacterial, fungal and viral pathogens causing various diseases among which, downy mildew caused by Hyaloperonospora brassicae, an oomycete is the most severe one. The present investigation was conducted to reduce fungicide load on Brassica by testing an alternate method of disease control. Different Systemic Acquired Resistance (SAR) compounds were tested as foliar sprays like Salicylic acid, Jasmonic acid and Bion (Benzothiadiazole-BTH) for inducing resistance in different genotypes of Raya (Brassica juncea) against downy mildew pathogen. Protein content in plants ranged between 43.5 to 57.7 mg/g fresh weight compared to 37.2 mg/g fresh weight in control. Induction of proteins and defense related enzymes was systemic in nature. The SAR compounds also surged the levels of defense related proteins, i.e. Polyphenol oxidase (PPO), Phenylalanine ammonia lyase (PAL) and pathogenesis related Pr- proteins i.e. ?-1,3 glucanase, Peroxidase (POD), from 21 to 130 per cent indicating induction of resistance. Protein profiling of treated Brassica plants was also done electrophoreticaly, which further confirmed the induction of pathogenesis-related proteins ranging from 15- 75 kDa along with some other proteins. Salicylic acid @ 500µM showed best results with 71.27 per cent disease control followed by Jasmonic acid with 69.6 per cent; whereas both, Bion and ?eta amino butyric acid gave almost 63 per cent disease control as compared to control plants. Integration of disease tolerance in Brassica varieties/genotypes combined with prophylactic spray of salicylic acid proved to be very economical for managing downy mildew disease.
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?-amino butyric acids (BABA), Benzothiadiazole (BTH), Brassica, Downy mildew, Jasmonic acid, Salicylic acid, Systemic acquired resistance
Astha, Sekhon, P. S. and Sangha, M. K. (2019). Influence of different SAR elicitors on induction and expression of PR-proteins in Potato and Muskmelon against Oomycete pathogens. Indian phytopath., 72, 43-51.
Barnes, J. D., Balaguer, L., Manrique, E., Elvira, S. and Davison, A. W. (1992). A reappraisal of the use of DMSO for extraction and determination of chlorophyll a and b in lichens and higher plants. Environ. Exp. Bot., 32, 85-100.
Boller, T. (1993). Antimicrobial function of the plant hydrolases, chitinase and ?-1, 3- glucanase. In: Firtig B and Legrand M (eds) Mechanisms of Plant Defense Responses, Pp 391-400, Kluwer academic publishers, Netherlands.
Burrell, M. M. and Rees, T. A. (1974). Metabolism of phenylalanine and tyrosine by rice leaves infected by Pyricularia oryzae. Physiol. Pl. Pathol., 4,497-508.
Cag, S., Cevahir, Oz. G., Sarsag, M. and Goren-saglam, N. (2009). Effect of salicylic acid on pigment, protein content and peroxidase activity in excised sunflower cotyledons. Pak. J. Bot., 41,2297-303.
Claiborne, S. and Fridovich, I. (1979). Assay for peroxidase. In: Sadasivan and Manickam .ed. Biochemical method Pp 190. New Age International Publisher New Delhi.
Conrath, U. (2011). Molecular aspects of defense priming. Trends. Pl. Sci., 16(10), 524-31.
Ding, C., Wang, C. Y., Gross, K. C. and Smith, D. L. (2002) Jasmonates and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to chilling injury in tomato fruit. Planta., 214, 895-901.
Durrant, W. E. and Dong, X. (2004). Systemic acquired resistance. Annu. Rev. Phytopathol., 42, 185-209.
Enkerli, JU., Gisi, E. and Mosinges, (1993). Systemic acquired resistance to Phytophora infestans in tomato and the role of pathogenesis-related proteins. Physiol. Mol. Plant. Pathol. 43, 161-171.
Farouk, S., Ghoneem, K. M. and Abeer, A. Ali. (2008). Induction and expression of systemic resistance to downy mildew diseases in cucumber by elicitors. Egypt. J. phytopath., 36, 95-111.
Gao, L. and Zhang, Y. (2013). Effect of Salicylic Acid on Pear Leaf Induced Resistance to Pear Ring Rot. Appl. Sci. J ., 22 (11), 1534-39.
Kauffman, S., Legrand, M., Geoffroy, P. and Frittig, B. (1987). Biological function of pathogenesis – related Proteins: four PR protein of tobacco have 1,3 beta -glucanase activity. EMBO. J ., 6 (11), 3209-12.
Keinath, A. P. (2015). Cucurbit downy mildew management for 2015. Clemson University Cooperative Extension, IL, 90.
Khodary, S. E. A. (2004). Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants. Int. J. Agric. Biol., 6, 5-8.
Kumar, P. R. (1999). Package of practices and contingency plan for enhancing production of rapeseed-mustard in India. A publ. of the National Res. Centre on Rapeseed-Mustard, Sewar, Bharatpur, pp 1-39.
Lowry, O. H., Rosebrough, N. J., Furr, A. L. and Randal, R. J. (1951). Protein measurement with folin-phenol reagent J. Boil. Chem., 193, 265-75.
Murthy, K. N., Uzma, F. and Srinivas, C. (2013). Induction of systemic resistance by Trichoderma asperellum against bacterial wilt of tomato caused by Ralstonia solanacearum. Ind.J.Agri.Res., 1, 181-94.
Oostendorp, M., Kunz, W., Dietrich, B. and Staub, T. (2001). Induced disease resistance in plants by chemical. Eur. J. Plant. Pathol., 107, 19 – 28.
Saharan, G. S., Verma, P.R. and Nashaat, N.I. (1997). Monograph on downy mildew of crucifers.technical bulletin 1997-01, Saskatoon Research Centre, Canada, 197 pp.
Sharma, N., Kaur, C., Singh, J. and Gupta, A. K. (2012). Biochemical responses associated with bacterial blight of rice (Oryza sativa). Arch. Phytopath. Pl. Protect., 45, 47-61.
Smith, J. A. and Hammerschmidt, R. (2007). Comparative study of acidic Peroxidases associated with induced resistance in cucumber, muskmelon and watermelon. Physiol. Mol. Plant. Pathol., 33 (2), 255-261.
Tian, S., Wang, Y., Qin, G. and Xu, Y. (2005). Induction of defense against alternaria rot by different elicitors in harvested pear fruit. Appl. Pl. Microbial. Biotechnol. 78,1-6.
Van Loon, L. C., Rep, M., Pieterse, C. M. (2006). Significance of inducible defense-related proteins in infected plants. Annu. Rev. Phytopathol., 44, 135-62.
Walker, J. M. (1996). SDS polyacrylamide gel electrophoresis of proteins. In, Walker J M (ed) The protein protocols handbook. pp 55-61. Humana Press Inc. Totowa N J.
Zauberman, G., Ronen, R., Akerman, M., Weksler, A., Rot, I. and Fuchs, Y. (1991). Post-harvest retention of the red colour of litchi fruit pericarp. Scientia. Hort. 47: 89-97
Ziadi, S., Barbedette, S., Godard, J. F., Monot, C., L,e Corre. D. and Silue, D. (2001). Production of pathogenesis related proteins in cauliflower (Brassica oleracea var botrytis) downy mildew (Peronosora sparasitica) pathosystems treated with acibenzollar -S- methyl. Pl. Pathol ., 50, 579-86.
Zimmerli, L., Jakab, C., Metraux, J. P. and Mauch-Mani, B. (2000). Potentiation of pathogen specific defense mechanisms in Arabidopsis by beta-aminobutyric acid. Proceedings of the national academy of sciences of the USA, 97,12929-25.
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