Assessment of anti bacterial, anti inflammation and wound healing activity in Wistar albino rats using green silver nanoparticles synthesized from Tagetes erecta leaves
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Abstract
Silver nanoparticles synthesized from plant material have superior bioactivities. The purpose of this current study was to synthesis, characterize and to explore the bioactive efficacy of silver nanoparticles (Ag-NPs) using aqueous leaf extract of Tageteserecta. The biosynthesized Ag-NPs were characterized using ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. Ag-NPs were studied for in-vivo anti-inflammatory and wound healing activities performed in female Wistar albino rats. UV –Vis absorption spectrum of the T.erecta leaves extract was obtained at 428nm due to excitation of surface plasmon vibration in nanoparticles and confirms the synthesis of silver nanoparticles. The FTIR analysis showed the presence of sulfate, alkene and alcohol in the AgNP of T.erectaleaves. The average crystallite size of AgNP synthesized was found to be 27.2 nm. The spherical silver grain of 15.5 nm average size has been depicted with high-resolution scanning electron microscopy. Maximum activity (15mm) of T.erecta leaves silver nanoparticles was observed against Salmonella typhi (15mm) followed by Escherichia coli (12mm). Ag-NPs exhibited significant wound healing activity and anti-inflammatory activity in carrageenan-induced paw volume tests performed in female Wistar albino rats. Colloidal Ag-NPs can be synthesized by simple, nonhazardous methods, and biosynthesized Ag-NPs using T.erectaleaves extract have significant therapeutic properties.This work evidently confirmed that silver nanoparticles mediated T.erecta could be considered as a potential source for anti-inflammatory and wound healing drug.
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Keywords
Anti-inflammatory activity, Bactericidal activity, Green synthesis, Silver nanoparticles, Tagetes erecta, Wound healing activity
References
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Baygar, T. Sarac, N., Ugur, A. and Karaca, I.R. (2018). Antimicrobial characteristics and biocompatibility of the surgical suturecoated with biosynthesized silver nanoparticles. Bioorg. Chem.,8 (6), 254–258.
Beyth, N. Houri Haddad, Y. Domb, A. Khan, W. and Hazan, R. (2015). Alternative antimicrobial approach: Nano-antimicrobial materials. Evid. Based Compl. Alt., 246012.
Blacklow, S.O., Li, J. Freedman, B.R. Zeidi, M. Chen, C. and Mooney, DJ.(2019). Bioinspired mechanically active adhesive dressings to accelerate wound closure. Science Advances, 5(7),3963.
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Fabrega, J. Renshaw, J.C. and Lead, J.R. (2009a). Interactions of silver nanoparticles with Pseudomonas putida biofilms. Environ Sci Technol., 43(23):9004–9009.
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Gopinath, V.Mubarakali, D. Priyadarshini, S. Priyadarshini, NM.Thajuddin, N. and Velusamy, P. (2012). Biosynthesis of Silver nanoparticles from Tribulus terrestris and its antimicrobial activity: a novel biological approach. Colloids and Surfaces, B: Biointerfaces, 96, 69-74.
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Hemali, P. Pooja,and M, Sumitra, C.(2015). Green synthesis of silver nanoparticles from marigold flower and its synergistic antimicrobial potential. Arab J Chem., 8(5),732–741.
HemaliPadalia, Pooja Moteriya, andSumitra Chanda.(2014). Green synthesis of silver nanoparticles from marigold flower and its synergist antimicrobial potential. Arabian Journal of Chemistry,10, 1878- 5352.
Jasminka Talapko, Tatjana Matijevic, Martina Juzbasic, Arlen AntolovicPozgain, and Ivana Skrlec (2020). Antibacterial activity of silver and its application in Dentistry, Cardiology and Dermatology.Microorganisms, 8,1400.
Kahalil, M.H. Ismail, E. H. and El-Magdob, F.(2012). Biosynthesis of Au nanoparticles using olive leaf extract. Arab. J. Chem., 5,431-437.
Kumar, SSD. Rajendran, NK. Houreld, NN. andAbrahamse, H.(2018). Recent advances on silver nanoparticle and biopolymer-based biomaterials for wound healing applications. Int. J. Biol. Macromol.,115,165–175.
Kumar, V. and Yadav, SK.(2009). Plant-mediated synthesis of silver and gold nanoparticles and their applications. Journal of Chemical Technology and Biotechnology, 84:151-157.
Liao, C. Li, Y. and Tjong, SC. (2019). Bactericidal and cytotoxic properties of silver nanoparticles. Int. J. Mol. Sci., 20,449.
Mathur, P. Jha, S. Ramteke, S.and Jain, NK. (2018). Pharmaceutical aspects of silver nanoparticles. Artif. Cells Nanomed. Biotechnol., 46, 115–126.
Mcdonnell, G. and Russell, AD. (1999). Antiseptics and disinfectants Activity, action, and resistance. Clin. Microbiol. Rev., 12, 147–179.
Mihai, MM. Dima, MB. Dima, B. and Holban, AM.(2019). Nanomaterials for wound healing and infection control. Materials Basel., 12(13),2176.
Mohanpuria, P. Rana, NK. and Yadav, SK.(2008). Biosynthesis of nanoparticles: technological concepts and future applications. Journal of Nanoparticle Research. 10:507-517.
Mubarak Ali, D. Thajuddin, N. Jeganathan, K. and Gunasekaran, M. (2011). Plant extracts mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens Colloids and Surfaces, B: Biointerfaces, 85, 360-365.
Nabikhan, A. Kandasamy, K. Raj, A.and Alikunhi, N. (2010). Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuviumportulacastrum. Colloids and Surfaces,B: Biointerfaces. 79:488-493.
Orlowski, P. Zmigrodzka, M. Tomaszewska, E. Ranoszek Soliwoda, K. Czupryn, M. Antos-Bielska, M. Szemraj, J. Celichowski, G. Grobelny, J. and Krzyzowska, M.(2018). Tannic acid-modified silver nanoparticles for wound healing: The importance of size. Int. J. Nanomed. 13, 991–1007.
Panacek, A.Kvitek, L.and Prucek, R.(2006). Silver colloid nanoparticles: synthesis, characterization and their antibacterial activity. J Phys Chem B., 110(33), 16248–16253.
Pothireddy, S.Kaliki, A. Mekapogu, AR.Yegireddy, M. Pagadala, E. P. and Prasad, TNVKV.(2016). Evaluation of the Wound Healing Efficacy of Chemical and Phytogenic Silver Nanoparticles. IET Nanobiotechnol., 10, 340–348.
Pourali, P.and Yahyaei, B. (2016). Biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat. J. Trace Elem. Med. Biol., 34, 22–31.
Pourali, P.Razavian Zadeh, N. andYahyaei, B. (2016). Silver nanoparticles production by two soil isolated bacteria, Bacillus thuringiensis and Enterobacter cloacae, and assessment of their cytotoxicity and wound healing effect in rats. Wound Repair Regen., 24, 860–869.
Prasad, TNVKV. Elumalai, EK.and Khateeja, S.(2011). Evaluation of the antimicrobial efficacy of phytogenic silver nanoparticles. Asian Pac J Trop Biomed., 82–85.
Prathna, TC. Chandrasekaran, N. Raichur, AM.and Mukherjee, A.(2011). Biomimetic synthesis of silver nanoparticles by Citrus limon (lemon) aqueous extract and theoretical prediction of particle size. Colloids and Surfaces, B: Biointerfaces., 82,152-159.
Reenal, M. andIruthayaKalai Selvam, S.(2015). Green Synthesis and Antibacterial activity of Silver Nanoparticles using Oryza Sativa Husk Extract. International Research Journal of Environment sciences., 4(5):68-72.
Satishkumar, M. Sneha, K. Won, SW. Cho, CW. Kim, S. and Yun, YS. (2009). Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its antibacterial activity.Colloids and Surfaces, B: Biointerfaces., 73:332-338.
Saxena, A. Tripathi, RM. Zafar, F.and Singh, P. (2012). Green synthesis of silver nanoparticles using aqueous solution of Ficus benghalensis leaf extract and characterization of their antibacterial activity. Materials Letters., 67, 91-94.
Shabnum Shaheen, Uzma Hanif, Farah Khan, Mehwish Jaffer, Sobia Ilyas, Zeb Siddique, Tehreema Iftikhar, Samina Sarwar, Sana Khalid and SaiqaIshtiaq.(2017). Study of Systematic Application of morpho palynological characterization of medicinal plants. Transylvanian Review, Vol 15,(19).
Shahverdi, AR.Fakhimi, A.Shahverdi, HR.and Minaian, S.(2007). Synthesis and effect of Silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. NanomedNanotechnol., 3(2),168–171.
Shang, L. Nienhaus, K. and Nienhaus, GU.(2014). Engineered nanoparticles interacting with cells: Size matters. J. Nanobiotechnol.12.
Sondi, I.and SalopekSondi, B. (2004).Silvernanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci., 275(1),177–182.
Tian, J. Wong, KKY. Ho, CM. Lok, CN. Yu, WY. Che, CM. Chiu, JF.and Tam, PKH.(2007). Topical delivery of silver nanoparticles promotes wound healing. Chem. Med. Chem., 2, 129–136.
Umesh, P. Dhuldhaj shivaji, D. Deshmukh Aniket, K. Gade, Madhu Yashpal and Mahendra, K.(2019).Tagetes erecta mediated phytosynthesis of silver nanoparticles: an ecofriendly Approach. Nusantara Bioscience, 11(1), 2087-3956.
Valli, JS. and Vaseeharan, B. (2012). Biosynthesis of silver nanoparticles by Cissus quadrangularis extracts. Materials Letters., 82, 171-173.
Vijayakumar, V. Samal, SK. Mohanty, and S. Nayak, SK.(2019). Recent advancements in biopolymer and metal nanoparticle-based materials in diabetic wound healing management. Int.J.Biol. Macromol., 122,137-148.
Wang, L. Hu, C.and Shao, L. (2017). The antimicrobial activity of nanoparticles: Present situation and prospects for the future. Int. J. Nanomed.,12,1227–1249.
Wei, L. Lu, J. Xu, H. Patel, A. Chen, ZS. and Chen, G.(2015). Silver nanoparticles: synthesis, properties and therapeutic applications. Drug. Discov. Today., 20,595-601.
Winter, CA.Risley,and EA.Nuss, GW.(1962). Carrageenan-induced edema in hind paw of rats as an assay for anti-inflammatory drugs. Proc. Soc. Exp. Biol. Med., 111, 544–547.
Wong, KKY. Cheung, SOF. Huang, L.Niu, J. Tao, C. Ho, CM. Che, CM.and Tam, PKH.(2009). Further evidence of the anti-inflammatory effects of silver nanoparticles. Chem. Med. Chem., 4,1129-1135.
Adebayo, E.A. Oloke, J.K. andAina, D.A. (2014) Bora TC. Antioxidant and nutritional importance of some Pleurotus species. JMBFS. 3(4):289-294.
Ahmad, N. Sharma, S.Alam, MK. Singh, VN. Shamsi, SF.andMehta, BR.(2010). Rapid synthesis of silver nanoparticles using dried medicinal plant of basil Colloids and Surfaces. B: Biointerfaces. 81:81-86.
Anil RamdasShet, Pritam Ghose, Laxmikant Patil, and VeerannaHombalimath. (2015). A Preliminary study on green synthesis and antibacterial activity of silver nanoparticles. Int. J. Curr. Biotechnol., 3(2), 1-6.
Bankar, A. Joshi, B. Kumar, A.R. and Zinjarde, S. (2010). Banana peel extract mediated novel route for the synthesis of silver nanoparticles. Colloids and Surfaces A-Physicochemical and Engineering Aspects, 368:58-63.
Baygar, T. Sarac, N., Ugur, A. and Karaca, I.R. (2018). Antimicrobial characteristics and biocompatibility of the surgical suturecoated with biosynthesized silver nanoparticles. Bioorg. Chem.,8 (6), 254–258.
Beyth, N. Houri Haddad, Y. Domb, A. Khan, W. and Hazan, R. (2015). Alternative antimicrobial approach: Nano-antimicrobial materials. Evid. Based Compl. Alt., 246012.
Blacklow, S.O., Li, J. Freedman, B.R. Zeidi, M. Chen, C. and Mooney, DJ.(2019). Bioinspired mechanically active adhesive dressings to accelerate wound closure. Science Advances, 5(7),3963.
Burdus, A.C.Gherasim, O.Grumezescu, A. M.Mogoanta, L.Ficai, A.and Andronescu, E. (2018). Biomedical applications of silver nanoparticles; An up-to-date overview. Nanomaterials, 8, 681.
Cameron, SJ. Hosseinian, F. and Willmore, WG.(2018). A current overview of the biological and cellular effects of nanosilver. Int. J. Mol. Sci. 19:2030.
Dakal, TC. Kumar, A. Majumdar, RS. and Yadav, V. (2016). Mechanistic basis of antimicrobial actions of silvernanoparticles. Front. Microbiol. 7l, 1831.
Deshmukh, SP. Patil, SM.Mullani,and SB.Delekar, SD.(2019). Silver nanoparticles as an effective disinfectant: A review. Mater.Sci.Eng.C Mater.97,954–965.
Devendra Jain, and Kothari, SL. (2014). Green synthesis of silver nanoparticles and their application in plant virus inhibition. J Mycol. Plant Pathol., 44:21-24.
Dixit Priyanka, Tripathi Shalini, Verma Kumar Navneetet.(2013). A brief study on marigold (Tagetes species): A review. International Research Journal of Pharmacy, 4(1), 43-47.
Fabrega, J. Fawcett, SR. Renshaw, JC.and Lead, JR.(2009b). Silver nanoparticle impact on bacterial growth: effect of pH, concentration and organic matter. Environ Sci Tech., 43(19),7285–7290.
Fabrega, J. Renshaw, J.C. and Lead, J.R. (2009a). Interactions of silver nanoparticles with Pseudomonas putida biofilms. Environ Sci Technol., 43(23):9004–9009.
Frankova, J.Pivodova, V. Vagnerova, H. Juranova, J.and Ulrichova, J.(2016). Effects of silver nanoparticles on primary cell cultures of fibroblasts and keratinocytes in a wound-healing model. J. Appl. Biomater. Funct. Mater.,14, 137-142.
Gong, C.P. Li. S.C. and Wang, R.Y.(2018). Development of biosynthesized silver nanoparticles-based formulation for treating wounds during nursing care in hospitals. J. Photochem. Photobiol. B., 183:137–141.
Gopinath, V.Mubarakali, D. Priyadarshini, S. Priyadarshini, NM.Thajuddin, N. and Velusamy, P. (2012). Biosynthesis of Silver nanoparticles from Tribulus terrestris and its antimicrobial activity: a novel biological approach. Colloids and Surfaces, B: Biointerfaces, 96, 69-74.
Haider, A.and Kang, IK. (2015). Preparation of silver nanoparticles and their industrial and biomedical applications:A comprehensive review. Adv. Mater. Sci. Eng.,16 5257.
Heatly NG. (1944). A method for the assay of pencillin. Biochem. J., 38,61-65.
Hemali, P. Pooja,and M, Sumitra, C.(2015). Green synthesis of silver nanoparticles from marigold flower and its synergistic antimicrobial potential. Arab J Chem., 8(5),732–741.
HemaliPadalia, Pooja Moteriya, andSumitra Chanda.(2014). Green synthesis of silver nanoparticles from marigold flower and its synergist antimicrobial potential. Arabian Journal of Chemistry,10, 1878- 5352.
Jasminka Talapko, Tatjana Matijevic, Martina Juzbasic, Arlen AntolovicPozgain, and Ivana Skrlec (2020). Antibacterial activity of silver and its application in Dentistry, Cardiology and Dermatology.Microorganisms, 8,1400.
Kahalil, M.H. Ismail, E. H. and El-Magdob, F.(2012). Biosynthesis of Au nanoparticles using olive leaf extract. Arab. J. Chem., 5,431-437.
Kumar, SSD. Rajendran, NK. Houreld, NN. andAbrahamse, H.(2018). Recent advances on silver nanoparticle and biopolymer-based biomaterials for wound healing applications. Int. J. Biol. Macromol.,115,165–175.
Kumar, V. and Yadav, SK.(2009). Plant-mediated synthesis of silver and gold nanoparticles and their applications. Journal of Chemical Technology and Biotechnology, 84:151-157.
Liao, C. Li, Y. and Tjong, SC. (2019). Bactericidal and cytotoxic properties of silver nanoparticles. Int. J. Mol. Sci., 20,449.
Mathur, P. Jha, S. Ramteke, S.and Jain, NK. (2018). Pharmaceutical aspects of silver nanoparticles. Artif. Cells Nanomed. Biotechnol., 46, 115–126.
Mcdonnell, G. and Russell, AD. (1999). Antiseptics and disinfectants Activity, action, and resistance. Clin. Microbiol. Rev., 12, 147–179.
Mihai, MM. Dima, MB. Dima, B. and Holban, AM.(2019). Nanomaterials for wound healing and infection control. Materials Basel., 12(13),2176.
Mohanpuria, P. Rana, NK. and Yadav, SK.(2008). Biosynthesis of nanoparticles: technological concepts and future applications. Journal of Nanoparticle Research. 10:507-517.
Mubarak Ali, D. Thajuddin, N. Jeganathan, K. and Gunasekaran, M. (2011). Plant extracts mediated synthesis of silver and gold nanoparticles and its antibacterial activity against clinically isolated pathogens Colloids and Surfaces, B: Biointerfaces, 85, 360-365.
Nabikhan, A. Kandasamy, K. Raj, A.and Alikunhi, N. (2010). Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuviumportulacastrum. Colloids and Surfaces,B: Biointerfaces. 79:488-493.
Orlowski, P. Zmigrodzka, M. Tomaszewska, E. Ranoszek Soliwoda, K. Czupryn, M. Antos-Bielska, M. Szemraj, J. Celichowski, G. Grobelny, J. and Krzyzowska, M.(2018). Tannic acid-modified silver nanoparticles for wound healing: The importance of size. Int. J. Nanomed. 13, 991–1007.
Panacek, A.Kvitek, L.and Prucek, R.(2006). Silver colloid nanoparticles: synthesis, characterization and their antibacterial activity. J Phys Chem B., 110(33), 16248–16253.
Pothireddy, S.Kaliki, A. Mekapogu, AR.Yegireddy, M. Pagadala, E. P. and Prasad, TNVKV.(2016). Evaluation of the Wound Healing Efficacy of Chemical and Phytogenic Silver Nanoparticles. IET Nanobiotechnol., 10, 340–348.
Pourali, P.and Yahyaei, B. (2016). Biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat. J. Trace Elem. Med. Biol., 34, 22–31.
Pourali, P.Razavian Zadeh, N. andYahyaei, B. (2016). Silver nanoparticles production by two soil isolated bacteria, Bacillus thuringiensis and Enterobacter cloacae, and assessment of their cytotoxicity and wound healing effect in rats. Wound Repair Regen., 24, 860–869.
Prasad, TNVKV. Elumalai, EK.and Khateeja, S.(2011). Evaluation of the antimicrobial efficacy of phytogenic silver nanoparticles. Asian Pac J Trop Biomed., 82–85.
Prathna, TC. Chandrasekaran, N. Raichur, AM.and Mukherjee, A.(2011). Biomimetic synthesis of silver nanoparticles by Citrus limon (lemon) aqueous extract and theoretical prediction of particle size. Colloids and Surfaces, B: Biointerfaces., 82,152-159.
Reenal, M. andIruthayaKalai Selvam, S.(2015). Green Synthesis and Antibacterial activity of Silver Nanoparticles using Oryza Sativa Husk Extract. International Research Journal of Environment sciences., 4(5):68-72.
Satishkumar, M. Sneha, K. Won, SW. Cho, CW. Kim, S. and Yun, YS. (2009). Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its antibacterial activity.Colloids and Surfaces, B: Biointerfaces., 73:332-338.
Saxena, A. Tripathi, RM. Zafar, F.and Singh, P. (2012). Green synthesis of silver nanoparticles using aqueous solution of Ficus benghalensis leaf extract and characterization of their antibacterial activity. Materials Letters., 67, 91-94.
Shabnum Shaheen, Uzma Hanif, Farah Khan, Mehwish Jaffer, Sobia Ilyas, Zeb Siddique, Tehreema Iftikhar, Samina Sarwar, Sana Khalid and SaiqaIshtiaq.(2017). Study of Systematic Application of morpho palynological characterization of medicinal plants. Transylvanian Review, Vol 15,(19).
Shahverdi, AR.Fakhimi, A.Shahverdi, HR.and Minaian, S.(2007). Synthesis and effect of Silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. NanomedNanotechnol., 3(2),168–171.
Shang, L. Nienhaus, K. and Nienhaus, GU.(2014). Engineered nanoparticles interacting with cells: Size matters. J. Nanobiotechnol.12.
Sondi, I.and SalopekSondi, B. (2004).Silvernanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci., 275(1),177–182.
Tian, J. Wong, KKY. Ho, CM. Lok, CN. Yu, WY. Che, CM. Chiu, JF.and Tam, PKH.(2007). Topical delivery of silver nanoparticles promotes wound healing. Chem. Med. Chem., 2, 129–136.
Umesh, P. Dhuldhaj shivaji, D. Deshmukh Aniket, K. Gade, Madhu Yashpal and Mahendra, K.(2019).Tagetes erecta mediated phytosynthesis of silver nanoparticles: an ecofriendly Approach. Nusantara Bioscience, 11(1), 2087-3956.
Valli, JS. and Vaseeharan, B. (2012). Biosynthesis of silver nanoparticles by Cissus quadrangularis extracts. Materials Letters., 82, 171-173.
Vijayakumar, V. Samal, SK. Mohanty, and S. Nayak, SK.(2019). Recent advancements in biopolymer and metal nanoparticle-based materials in diabetic wound healing management. Int.J.Biol. Macromol., 122,137-148.
Wang, L. Hu, C.and Shao, L. (2017). The antimicrobial activity of nanoparticles: Present situation and prospects for the future. Int. J. Nanomed.,12,1227–1249.
Wei, L. Lu, J. Xu, H. Patel, A. Chen, ZS. and Chen, G.(2015). Silver nanoparticles: synthesis, properties and therapeutic applications. Drug. Discov. Today., 20,595-601.
Winter, CA.Risley,and EA.Nuss, GW.(1962). Carrageenan-induced edema in hind paw of rats as an assay for anti-inflammatory drugs. Proc. Soc. Exp. Biol. Med., 111, 544–547.
Wong, KKY. Cheung, SOF. Huang, L.Niu, J. Tao, C. Ho, CM. Che, CM.and Tam, PKH.(2009). Further evidence of the anti-inflammatory effects of silver nanoparticles. Chem. Med. Chem., 4,1129-1135.
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How to Cite
Assessment of anti bacterial, anti inflammation and wound healing activity in Wistar albino rats using green silver nanoparticles synthesized from Tagetes erecta leaves. (2021). Journal of Applied and Natural Science, 13(1), 343-351. https://doi.org/10.31018/jans.v13i1.2519
