Molecular characterization of Indian species of the genus Cornudiscoides Kulkarni, 1969 (Monogenoidea: Dactylogyridae)
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Abstract
Molecular characterization and phylogenetic study based on partial sequences of 28S and 18S ribosomal DNA (rDNA) of sixteen Indian species of the genus Cornudiscoides (Monogenoidea: Dactylogyridae) were conducted to decode the genetic relationship between them and with other members of the family Dactylogyridae. Blastn searches disclosed the significant similarity among the species of the Cornudiscoides for large ribosomal subunits as well as for small ribosomal subunit showing genetic relatedness. The phylogenetic tree using neighbour-joining (NJ) and minimum evolution (ME) methods for 28S ribosomal subunit depicted that all Cornudiscoides species clustering in a single clade and forming sister clade with other members of the family Dactylogyridae and similar results were obtained from 18S ribosomal subunit. Thus, the present study demonstrated that both 28S and 18S ribosomal subunits are very helpful in discriminating Cornudiscoides species (intra or interspecific variation) and in the establishment of the evolutionary relationship among them.
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Keywords
Cornudiscoides, Dactylogyridae, Large and small ribosomal subunit, Maximum likelihood methods, Neighbour-joining, Phylogenetic analysis
References
Agrawal, N., Verma, J., Rajvanshi, S. & Asthana, A. (2020). Application of two interesting statistical tools, used in differentiation of closely related species of Cornudiscoides Kulkarni, 1969 (Platyhelminthes: Monogenoidea: Dactylogyridae). Uttar Pradesh Journal of Zoology, 41(10) ,143-156.
Agrawal, N., Rajvanshi, S. & Verma, J. (2016). Two new species of the genus Cornudiscoides Kulkarni, 1969 from naked catfish Sperata aor (Hamilton, 1822): Specialist or Generalist? Pakistan Journal of Zoology, 48 (6) , 1687-1693.
Agrawal, N. & Vishwakarma, P. (1996). Six new species and re-description of two known species of the genus Cornudiscoides Kulkarni, 1969 (Monogenea) from Lucknow U.P. Indian Journal of Helmintology, 13 (1-2) , 10-31
Blair, D. & Barker, S.C. (1993). Affinities of the Gyliauchenidae: utility of 18S rRNA gene for phylogenetic inference in the Digenea (Platyhelminthes). International Journal for Parasitology, 23(4), 527–532. doi.org/10.10 16/0020-7519(93)90042-W
Cunningham, C.O., Aliesky, H. & Collins, C.M. (2000). Sequence and secondary structure variation in the Gyrodactylus (Platyhelminth: Monogenea) ribosomal RNA gene array. Journal of Parasitology, 83 (3), 567–576.doi.org/10.1645/0022-3395(2000) 086[0567:SASSVI] 2.0.CO;2
Cunningham, C.O., McGillivary, D.M. & Mackenze, K. (1995). Phylogenetic analysis of Gyrodactylus salaries Malmberg, 1957 based on the small subunit (18S) ribosomal RNA gene. Molecular and Biochemical Parasitology, 71(1) 139–142. doi: 10.1016/0166-6851(95)00043-z
Dayrat, B. (2005). Towards integrative taxonomy. Biological Journal of Linnean Society, 85(3) , 407–415. doi.org/10.1111/j.1095-8312.2005.00503.x
Devak, A. & Pandey, K.C. (2007). A new species of
Cornudiscoides Kulkarni, 1969 (Monogenoidea: Dactylogyridae) its locomotion, mode of attachment and distribution. Indian Journal of Helminthology, 25 (1-2), 41-58.
Dubey, A. Gupta, A.K. & Agrawal, S.M. (1992). Studies on monogenean parasites in freshwater fishes at Raipur IX. Two new species of the genus Cornudiscoides Kulkarni,1969 and a taxonomic discussion on species included in it. Indian Journal of Helminthology, 44, 109-115.
Ferguson, J. W. H. (2002). On the use of genetic divergence for identifying species. Biological journal of the Linnean Society, 75(4), 509-516.
Froese, R. and Pauly, D. (2014-2018) Fish base. World Wide Web electronic publication. Version 10/2015. Available at www.fishbase.org (accessed).
Gusev, A.V. (1976). Freshwater Indian Monogenoidea. Principles of systematics, analysis of the world faunas and their evolution. Indian Journal of Helminthology, 25 and 26, 1–241.
Hillis, DM., & Dixon, MT. (1991). Ribosomal DNA molecular evolution and phylogenetic inference. Quarterly Review of Biology, 66 (4), 411-453.doi: 10.1086/417338
Jayaram, K.C. (1955). Silurid fishes of India, Burma and Ceylon. XIV: Fishes of the genus Mystus Scopoli, 1777. Record Indian Museum (Culcutta), 51, 527–558.
Kritsky, DC., Thatcher, VE. & Boeger, WA. (1986). Neotropical Monogenea 8. Reveision of Urocleidoides (Dactylogyridae, Ancyrocephalinae). Proceding of
Helminthological Society of Washington, 53 (1) , 1-37.
Kulkarni, T. (1969). Studies on the monogenetic trematodes of fishes found in Hyderabad, Andhra Pradesh (India). Part I. Riv di Parasitologica 30 (2), 73–90.
Kumar, S., Stecher, G. & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology Evolution, 33(7) , 870-1874. doi:10.1093/molbev/msw054. doi: 10.1093/molbev/msw054.
Littlewood, D.T., Rohde, K. & Clough, K.A. (1998). The phylogenetic position of Udonella (Platyhelminth). International Journal of Parasitology, 28 (8), 1241-1250. doi: 10.1016/s0020-7519(98)00108-8.
Matejusová, I., Koubková, B., Amelio, S.D. & Cunniungham, C.O. (2001). Genetic characterization of six species of Diplozoon (Monogenea; Dioplozoiae). Parasitology, 123 (5), 465–474. doi: 10.1017/s0031182001008617.
Mollaret, I., Jamieson, B.G.M. & Justine, J.L. (2000). Phylogeny of Monopisthocotylea and Polyopisthocotylea (Platyhelminthes) inferred from 28S rDNA sequences. International Journal of Parasitology, 30 (2), 171–185. doi:10.1016/S0020-7519(99)00197-6.
Mollaret, I., Jamieson, B.G.M., Adlard, R.D., Hugall, A., Lecointre, G., Chombard, C. & Justine, J.L. (1997). Phylogenetic analysis of the Monogenea and their relationships with Digenea and Eucestoda inferred from 28S rDNA sequences. Molecular and Biochemical Parasitology, 90(2), 433–438. doi: 10.1016/S0166-6851(97)00176-X.
Nolan, M. & Cribb, T. (2005). The use and implication of ribosomal DNA sequencing for the discrimination of dignean species. Advances in Parasitology, 60,101-163. doi: 10.1016/S0065-308X(05)60002-4
Olson, P. & Littlewood D.T.G. (2002). Phylogeny of Monogenea – evidence from a medley of molecules. International Journal for Parasitology, 32(3), 233–244. doi.org/10.1016/S0020-7519 (01)00328-9
Olsen, G.J. & Woese, C.R. (1993). Ribosomal RNA: a key to phylogeny. FASEB J 7, 113-123. doi:10.1096/fase bj.7.1.8422957
Pandey, K.C. & Agrawal, N. (2008). An encyclopaedia of Indian Monogenoidea. New Delhi, Vitasta Publishing.
Plaisance, L., Littlewood, D.T.J., Olson, P.D. & Morand, S. (2005). Molecular phylogeny of gill monogeneans (Platyhelminthes, Monogenea, Dactylogyridae) and colonization of Indo-West Pacific butterflyfish hosts (Perciformes, Chaetodontidae). Zoologica Scripta, 34 (4), 425–436. doi.org/10.1111/j.1463-6409.2005.00191.x.
Rizvi, S.S.H. (1971). Monogenea of Pakistan fishes I. Ancylodiscoides mystusi, New species and A. aori, New species, from the gills of Mystus aor (Ham.). Pakistan Journal of Zoology, 3, (2)87-92.
Schlick-Steiner, B.C., Steiner, FM. Seifert, B. Stauffer, C. Christian, E. & Crozier, RH. (2010). Integrative taxonomy: a multisource approach to exploring biodiversity. Annual Review of Entomology, 55, 421–438. doi.org/10.1146/an nurev-ento-112408-085432.
Šimková, A., Matejusová, I. & Cunniungham, C.O. (2006). A molecular phylogeny of the Dactylogyridae sensu Kristy and Boeger (1998) (Monogenea) based on the D1–D3 domain of larger subunit of rDNA. Parasitology, 133(1) , 43–53. doi: 10.1017/S0031182006009942.
Šimková, A., Plaisance, L., Matejusová, I., Morand, S. & Verneau, O. (2003). Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933 (Monogenea: Dactylogyridea): the need for the systematic revision of the Ancyrocephalinae Bychowsky, 1937. Systematic Parasitology, 54(1), 1–11.doi.org/10.1023/A:1022133608662.
Shrivastava, R.R., Agrawal, N. & Upadhyay, M.K. (2013). Molecular analysis based on 28S rDNA of Dactylogyroides species parasitizing Puntius species. Bioengineering and Biosciences, 1(3), 25–30. doi: 10.13189/bb.20 13.010301
Van Steenberge, M., Pariselle, A., Huyse, T., Volckaert, F. A., Snoeks, J., & Vanhove, M. P. (2015). Morphology, molecules, and monogenean parasites: an example of an integrative approach to cichlid biodiversity. PloS one, 10, e0124474. doi.org/10.1371/journal.pone.0124474
Verma, J., Agrawal, N. & Verma, A.K. (2016). The use of large and small subunits of ribosomal DNA in evaluating phylogenetic relationships between species of Cornudiscoides Kulkarni, 1969 (Monogenoidea: Dactylogyridae) from India. Journal of Helminthology, 91(2) , 206-214. doi: 10.1017/S0022149X16000134
Verma J., Rajvanshi S. & Agrawal, N. (2018). Genetic characterization of three species of the genusCornudiscoides Kulkarni, 1969 (Monogenoidea: Dactylogyridae), Parasitizing long whiskered cat fish Sperata aor (Ham) using ribosomal and mitochondrial DNA. Journal of Zoological Sciences, 6, 31-37.
Zhu, X., Gasser, R.B. & Chilton, N.B. (1998). Differences in the 5.8S rDNA sequences among ascarid nematodes. International Journal for Parasitology, 28(4), 617–622. doi.org/10.1016/S0020-7519(97)00214-2.
Agrawal, N., Rajvanshi, S. & Verma, J. (2016). Two new species of the genus Cornudiscoides Kulkarni, 1969 from naked catfish Sperata aor (Hamilton, 1822): Specialist or Generalist? Pakistan Journal of Zoology, 48 (6) , 1687-1693.
Agrawal, N. & Vishwakarma, P. (1996). Six new species and re-description of two known species of the genus Cornudiscoides Kulkarni, 1969 (Monogenea) from Lucknow U.P. Indian Journal of Helmintology, 13 (1-2) , 10-31
Blair, D. & Barker, S.C. (1993). Affinities of the Gyliauchenidae: utility of 18S rRNA gene for phylogenetic inference in the Digenea (Platyhelminthes). International Journal for Parasitology, 23(4), 527–532. doi.org/10.10 16/0020-7519(93)90042-W
Cunningham, C.O., Aliesky, H. & Collins, C.M. (2000). Sequence and secondary structure variation in the Gyrodactylus (Platyhelminth: Monogenea) ribosomal RNA gene array. Journal of Parasitology, 83 (3), 567–576.doi.org/10.1645/0022-3395(2000) 086[0567:SASSVI] 2.0.CO;2
Cunningham, C.O., McGillivary, D.M. & Mackenze, K. (1995). Phylogenetic analysis of Gyrodactylus salaries Malmberg, 1957 based on the small subunit (18S) ribosomal RNA gene. Molecular and Biochemical Parasitology, 71(1) 139–142. doi: 10.1016/0166-6851(95)00043-z
Dayrat, B. (2005). Towards integrative taxonomy. Biological Journal of Linnean Society, 85(3) , 407–415. doi.org/10.1111/j.1095-8312.2005.00503.x
Devak, A. & Pandey, K.C. (2007). A new species of
Cornudiscoides Kulkarni, 1969 (Monogenoidea: Dactylogyridae) its locomotion, mode of attachment and distribution. Indian Journal of Helminthology, 25 (1-2), 41-58.
Dubey, A. Gupta, A.K. & Agrawal, S.M. (1992). Studies on monogenean parasites in freshwater fishes at Raipur IX. Two new species of the genus Cornudiscoides Kulkarni,1969 and a taxonomic discussion on species included in it. Indian Journal of Helminthology, 44, 109-115.
Ferguson, J. W. H. (2002). On the use of genetic divergence for identifying species. Biological journal of the Linnean Society, 75(4), 509-516.
Froese, R. and Pauly, D. (2014-2018) Fish base. World Wide Web electronic publication. Version 10/2015. Available at www.fishbase.org (accessed).
Gusev, A.V. (1976). Freshwater Indian Monogenoidea. Principles of systematics, analysis of the world faunas and their evolution. Indian Journal of Helminthology, 25 and 26, 1–241.
Hillis, DM., & Dixon, MT. (1991). Ribosomal DNA molecular evolution and phylogenetic inference. Quarterly Review of Biology, 66 (4), 411-453.doi: 10.1086/417338
Jayaram, K.C. (1955). Silurid fishes of India, Burma and Ceylon. XIV: Fishes of the genus Mystus Scopoli, 1777. Record Indian Museum (Culcutta), 51, 527–558.
Kritsky, DC., Thatcher, VE. & Boeger, WA. (1986). Neotropical Monogenea 8. Reveision of Urocleidoides (Dactylogyridae, Ancyrocephalinae). Proceding of
Helminthological Society of Washington, 53 (1) , 1-37.
Kulkarni, T. (1969). Studies on the monogenetic trematodes of fishes found in Hyderabad, Andhra Pradesh (India). Part I. Riv di Parasitologica 30 (2), 73–90.
Kumar, S., Stecher, G. & Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Molecular Biology Evolution, 33(7) , 870-1874. doi:10.1093/molbev/msw054. doi: 10.1093/molbev/msw054.
Littlewood, D.T., Rohde, K. & Clough, K.A. (1998). The phylogenetic position of Udonella (Platyhelminth). International Journal of Parasitology, 28 (8), 1241-1250. doi: 10.1016/s0020-7519(98)00108-8.
Matejusová, I., Koubková, B., Amelio, S.D. & Cunniungham, C.O. (2001). Genetic characterization of six species of Diplozoon (Monogenea; Dioplozoiae). Parasitology, 123 (5), 465–474. doi: 10.1017/s0031182001008617.
Mollaret, I., Jamieson, B.G.M. & Justine, J.L. (2000). Phylogeny of Monopisthocotylea and Polyopisthocotylea (Platyhelminthes) inferred from 28S rDNA sequences. International Journal of Parasitology, 30 (2), 171–185. doi:10.1016/S0020-7519(99)00197-6.
Mollaret, I., Jamieson, B.G.M., Adlard, R.D., Hugall, A., Lecointre, G., Chombard, C. & Justine, J.L. (1997). Phylogenetic analysis of the Monogenea and their relationships with Digenea and Eucestoda inferred from 28S rDNA sequences. Molecular and Biochemical Parasitology, 90(2), 433–438. doi: 10.1016/S0166-6851(97)00176-X.
Nolan, M. & Cribb, T. (2005). The use and implication of ribosomal DNA sequencing for the discrimination of dignean species. Advances in Parasitology, 60,101-163. doi: 10.1016/S0065-308X(05)60002-4
Olson, P. & Littlewood D.T.G. (2002). Phylogeny of Monogenea – evidence from a medley of molecules. International Journal for Parasitology, 32(3), 233–244. doi.org/10.1016/S0020-7519 (01)00328-9
Olsen, G.J. & Woese, C.R. (1993). Ribosomal RNA: a key to phylogeny. FASEB J 7, 113-123. doi:10.1096/fase bj.7.1.8422957
Pandey, K.C. & Agrawal, N. (2008). An encyclopaedia of Indian Monogenoidea. New Delhi, Vitasta Publishing.
Plaisance, L., Littlewood, D.T.J., Olson, P.D. & Morand, S. (2005). Molecular phylogeny of gill monogeneans (Platyhelminthes, Monogenea, Dactylogyridae) and colonization of Indo-West Pacific butterflyfish hosts (Perciformes, Chaetodontidae). Zoologica Scripta, 34 (4), 425–436. doi.org/10.1111/j.1463-6409.2005.00191.x.
Rizvi, S.S.H. (1971). Monogenea of Pakistan fishes I. Ancylodiscoides mystusi, New species and A. aori, New species, from the gills of Mystus aor (Ham.). Pakistan Journal of Zoology, 3, (2)87-92.
Schlick-Steiner, B.C., Steiner, FM. Seifert, B. Stauffer, C. Christian, E. & Crozier, RH. (2010). Integrative taxonomy: a multisource approach to exploring biodiversity. Annual Review of Entomology, 55, 421–438. doi.org/10.1146/an nurev-ento-112408-085432.
Šimková, A., Matejusová, I. & Cunniungham, C.O. (2006). A molecular phylogeny of the Dactylogyridae sensu Kristy and Boeger (1998) (Monogenea) based on the D1–D3 domain of larger subunit of rDNA. Parasitology, 133(1) , 43–53. doi: 10.1017/S0031182006009942.
Šimková, A., Plaisance, L., Matejusová, I., Morand, S. & Verneau, O. (2003). Phylogenetic relationships of the Dactylogyridae Bychowsky, 1933 (Monogenea: Dactylogyridea): the need for the systematic revision of the Ancyrocephalinae Bychowsky, 1937. Systematic Parasitology, 54(1), 1–11.doi.org/10.1023/A:1022133608662.
Shrivastava, R.R., Agrawal, N. & Upadhyay, M.K. (2013). Molecular analysis based on 28S rDNA of Dactylogyroides species parasitizing Puntius species. Bioengineering and Biosciences, 1(3), 25–30. doi: 10.13189/bb.20 13.010301
Van Steenberge, M., Pariselle, A., Huyse, T., Volckaert, F. A., Snoeks, J., & Vanhove, M. P. (2015). Morphology, molecules, and monogenean parasites: an example of an integrative approach to cichlid biodiversity. PloS one, 10, e0124474. doi.org/10.1371/journal.pone.0124474
Verma, J., Agrawal, N. & Verma, A.K. (2016). The use of large and small subunits of ribosomal DNA in evaluating phylogenetic relationships between species of Cornudiscoides Kulkarni, 1969 (Monogenoidea: Dactylogyridae) from India. Journal of Helminthology, 91(2) , 206-214. doi: 10.1017/S0022149X16000134
Verma J., Rajvanshi S. & Agrawal, N. (2018). Genetic characterization of three species of the genusCornudiscoides Kulkarni, 1969 (Monogenoidea: Dactylogyridae), Parasitizing long whiskered cat fish Sperata aor (Ham) using ribosomal and mitochondrial DNA. Journal of Zoological Sciences, 6, 31-37.
Zhu, X., Gasser, R.B. & Chilton, N.B. (1998). Differences in the 5.8S rDNA sequences among ascarid nematodes. International Journal for Parasitology, 28(4), 617–622. doi.org/10.1016/S0020-7519(97)00214-2.
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Molecular characterization of Indian species of the genus Cornudiscoides Kulkarni, 1969 (Monogenoidea: Dactylogyridae). (2021). Journal of Applied and Natural Science, 13(1), 1-7. https://doi.org/10.31018/jans.v13i1.2434
