Development of a web-based tool for probit analysis to compute LC50/LD50/GR50 for its use in toxicology studies

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Published Dec 9, 2020
DOI: https://doi.org/10.31018/jans.v12i4.2408

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Vinay Kumar
Department of Mathematics and Statistics, CCS Haryana Agricultural University, Hisar -125004 (Haryana), India
O.P. Sheoran
Department of Mathematics and Statistics, CCS Haryana Agricultural University, Hisar -125004 (Haryana), India
Sarita Rani
Department of Mathematics and Statistics, CCS Haryana Agricultural University, Hisar -125004 (Haryana), India
Komal Malik
Department of of Economics, Govt. College, Nalwa, Hisar (Haryana), India

Abstract

The toxicity bioassays are essential to detect and estimate the potential toxicological effects of chemicals on an organism.
LC50/LD50/GR50 is the estimation of dose/concentration necessary to kill 50 per cent of a population of the test species. Experimentally this is done by administrating a chemical at different doses to a group of organism and then observing the resulting mortalities in a set of the time period. A web-based module for a statistical analysis tool to calculate and compare the median lethal dose has been developed in ASP scripting language based on Server-Client Architecture. The module produces the final probit line, dose-response curve, LC50/LD50 with 95 % confidence interval. The Chi-square statistic has been obtained for testing the adequacy of fit. A user-friendly interface for entering/pasting the data and various parameters such as number of variables, number of observations etc. has also been provided. Additionally, a complete procedure to perform probit analysis has also been provided in the help file.

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Keywords

Concentration, LC50/LD50, Probit Analysis, Server-Client Architecture

References
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Issue
Vol 12 No 4 (2020)
Section
Research Articles
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How to Cite

Development of a web-based tool for probit analysis to compute LC50/LD50/GR50 for its use in toxicology studies. (2020). Journal of Applied and Natural Science, 12(4), 641-646. https://doi.org/10.31018/jans.v12i4.2408