The genotoxic effects of nimbecidine, a commercial botanical pesticide derived from the neem tree were assessed by PCR assay on a mosquito Anopheles stephensi taken as an experimental model. After treatment with LC20 of the nimbecidine, the sequence variations in the internal transcribed spacers 1 and 2 (ITS1 and ITS2) of control and treated individuals were studied from their sequence alignment data and the mutations in the form of insertions, deletions, and substitutions were analyzed. Nimbecidine treatment induced 16 deletions, 13 insertions, 93 transitions and 140 transversions in the ITS1 sequence. Similarly, in the ITS2 sequence of treated individual there were 2 deletions, 4 insertions, 15 transitions and 39 transversions. Present study suggests that plant based pesticides also effect the integrity of normal DNA sequences.
Anopheles stephensi, Nimbecidine genotoxicity, ITS1 and 2
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