Antioxidant activity and production of secondary metabolites of adult plant and in vitro calli of Anodendron paniculatum
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Abstract
The members of the family Apocynaceae are the rich in alkaloids, terpinoids, flavonoids, phenolic compounds and hydrocarbons. Anodendron paniculatum Roxb. is an woody climber, the roots of which have been used to control vomiting and cough. In the present study, adult plant and tissue culture plant extracts of A. paniculatum collected from Eastern Ghats, Araku, Andhra Pradesh were used for the production of their secondary metabolites and antioxidant activity. Methanol extracts of calli showed high contents of total phenol and alkaloid with 25.53 and 25.98 mg/g respectively. Methanol extract of tissue culture plant at higher concentrations showed better antioxidant activity with reference to standard ascorbic acid. For this study different concentrations (12.5, 25, 50, 100 and 200 µg/ml) of extracts were prepared by using four different solvents. Tissue culture extracts from methanol showed maximum scavenging activity with 231.9±1.39 % at 200 µg/ml of concentration, while that of adult plant extracts showed the scavenging activity of 189.1±0.74% only. These results on high antioxidant activity of the methanol extract of A. paniculatum calli can be attributed to the presence of high phenolic and alkaloid contents in calli when compared to that of adult plant extract.
Article Details
Article Details
Alkaloids, Anodendron paniculatum, Antioxidant activity, Apocynaceae, Phenols
Anbukkarasi, M., Thomas, P. A., Sundararajan, M., and Geraldine, P. (2016). Gas chromatography?mass spectrometry analysis and in vitro antioxidant activity of the ethanolic extract of the leaves of Tabernaemontana divaricata. Pharmacognosy Journal, 8(5) : 451–458.
Bhadane, B. S., & Patil, R. H. (2017). Isolation, purification and characterization of antioxidative steroid derivative from methanolic extract of Carissa carandas (L.) leaves. Biocatalysis and Agricultural Biotechnology, 10, 216–223.
Bidchol, A.M., Wilfred, A., Abhijna, P. and Harish, R., (2011). Free radical scavenging activity of aqueous and ethanolic extract of Brassica oleracea L. var. italica. Food and Bioprocess Technology, 4(7):1137-1143. DOI 10.1007/s11947-009-0196-9
Chi, V.V. (1997). Dictionary of Vietnam Medicinal Plants. Medical Publishing House, Hanoi.
Elnour, M.E., Ali, A.M. and Saeed, B.A.T. (2015). Effect of different concentrations of auxins and combination with kinetin on callus initiation of Trigonella Foenum-Graecum. L. Int. J. Technic. Res. Appl, 3(2):117-122.
Flora of China Editorial Committee (1995). Flora of China (Gentianaceae through Boraginaceae). Volume 16. Flora of China Press & Missouri Botanical Garden Press, Beijing and St.Louis.
Ganapathy, P. S., Ramachandra, Y. L., & Rai, S. P. (2011). In vitro antioxidant activity of Holarrhena antidysenterica Wall. methanolic leaf extract. Journal of Basic and Clinical Pharmacy, 4(4), 175.
Harborne, J.B. and Williams, C.A. (2000). Advances in flavonoid research since 1992. Phytochemistry 55 : 481-504. https://doi.org/10.1016/S0031-9422(00)00235-1
Iqbal, Z., Iqbal, M.S. and Mishra, K., (2017). Screening of antioxidant property in medicinal plants belonging to the family Apocynaceae. Asian J Pharm Clin Res, 10(12):415-418. DOI: http://dx.doi.org/10.22159/ajpcr.2017.v10i12.22303
James, J., Veettil, A. K. T., Pratyush, K., Misra, C. S., Sahadevan, L. D. M., & Thankamani, V. (2011). In vitro antioxidant activity of flowers and fruits of Alstonia scholaris. International Journal of Phytomedicine, 3(4), 475– 479.
Middleton DJ., Flora Malesiana. (2007). Series I, Volume 18. Foundation Flora Malesiana, Netherlands.
Shamsa, F., Monsef, H., Ghamooshi, R. and Verdian-rizi, M., (2008). Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci., 32:17-20.
Singleton, V.L., Orthofer, R. and Lamuela-Raventós, R.M., (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In Methods in Enzymology, 299: 152-178. https://doi.org/10.1016/S0076-6879(99)99017-1
Takhtajan, A. (2009). Flowering plants. Springer, St. Petersburg.
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