An effort has been made to propagate Dendrocalamus strictus Nees using nodal explants from mature plants. The explants, after surface sterilization, were inoculated on solidified MS basal medium with 2 mg/l BAP. The buds, which started growing, were transferred to solidified MS basal medium with 4 mg/l BAP and 15 mg/l AdS to achieve about three fold multiplication. Single shoots or 2-shoot clusters did not survive and therefore all the experiments were conducted with a cluster of minimum 3-shoots. The rooting was found difficult. Three hormones viz. IAA, IBA and NAA with a concentration of 1, 3 and 5 mg/l were used in various combinations for rooting. Out of the total 9 treatments, only 20% rooting was found only in one treatment, namely in the medium with 5 mg/l indole butyric acid (IBA).
Bamboo, Dendrocalamus strictus, Micropropagation, Nodal explants
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