Micropropagation of Dendrocalamus strictus nees from mature nodal explants
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Abstract
An effort has been made to propagate Dendrocalamus strictus Nees using nodal explants from mature plants. The explants, after surface sterilization, were inoculated on solidified MS basal medium with 2 mg/l BAP. The buds, which started growing, were transferred to solidified MS basal medium with 4 mg/l BAP and 15 mg/l AdS to achieve about three fold multiplication. Single shoots or 2-shoot clusters did not survive and therefore all the experiments were conducted with a cluster of minimum 3-shoots. The rooting was found difficult. Three hormones viz. IAA, IBA and NAA with a concentration of 1, 3 and 5 mg/l were used in various combinations for rooting. Out of the total 9 treatments, only 20% rooting was found only in one treatment, namely in the medium with 5 mg/l indole butyric acid (IBA).
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Keywords
Bamboo, Dendrocalamus strictus, Micropropagation, Nodal explants
References
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Arya, S. and Sharma, S. (1998). Micropropagation technology of Bambusa bambos through shoot proliferation. Ind. For., 9: 725-731.
Arya, S., Rana, P. K., Sharma, R. and Arya, I. D. (2006). Tissue culture technology for rapid multiplication of Dendrocalamus giganteus Munro. Ind. For., 132: 345-357.
Banik, R. L. (1987). Techniques of bamboo propagation with special reference to pre-rooted and pre-rhizomed branch cutting and tissue culture. In: A. N. Rao, G. Dhanraj and C.
B. Shastry (Eds.), Recent research on bamboos, The Chinese Acad. For. and IDRC, Singapore.
Chambers, S. M., Heuch, J. H. R. and Pirrie, A. (1991). Micropropagation and in vitro flowering of the bamboo Dendrocalamus hamiltonii Munro. Plant Cell Tiss. Org. Cult., 27: 45-48.
Chaturvedi, A. N. (1986). Bamboos for farming. Uttar Pradesh Forest Bulletin, No.52., Lucknow.
Chaturvedi, H. C., Sharma, M. and Sharma, A. K. (1993). In vitro regeneration of Dendrocalamus strictus Nees. through nodal segments taken from field-grown culms. Plant Sci., 92: 97-101.
Das, P. and Rout, G. R. (1991). Mass multiplication and flowering of bamboo in vitro. Orissa J. Hort., 19: 118-121.
Dekkers, A. J. and Rao, A. N. (1989). Tissue culture of four bamboo genera. In: A. N. Rao and A. M. Yusoff (Eds.), Proc. Seminar on tissue culture of forest species. FRI, Malaysia and IDRC, Singapore.
George, E. F. (1993). Plant propagation by tissue culture, Vol. I. Exegetics Ltd., Edington, England.
Khoshoo, T. N. (1994). Making forestry sustainable. Curr Sci., 70: 205-214.
Maity, S. and Ghosh, A. (1997). Efficient plant regeneration from seeds and nodal segments of Dendrocalamus strictus using in vitro techniques. Ind For., 4: 313-318.
Nadgir, A. L., Phadke, C. H., Gupta, P. K., Parshrami, V. A., Nair, S. and Mascarenhas, A. F. (1984). Rapid multiplication of bamboo by tissue culture. Silvae Genetica, 33: 219-223.
Paranjothy, K., Saxena, S., Banerjee, M., Jaiswal, V. S. and Bhojwani, S. S. (1990). Clonal multiplication of woody plants. In: S. S. Bhojwani (Ed.), Plant Tissue Culture: Application and Limitations. Elsevier, Amsterdam.
Pathak, K. C., Sharma, A. K., Hazarika, P. K., Neog, D. and Mishra, H. (2005). Genetic conservation and improvement work of bamboos at Rain Forest Research Institute, Jorahat, Assam. Ind For., 131: 11.
Prutpongse, P. and Gavinlertvatana, P. (1992). In vitro micropropagation of 54 species from 15 genera of bamboo. Hort. Sci, 27: 453-454.
Rao, I. U., Rao, I. V. R. and Narang, V. (1985). Somatic embryogenesis and regeneration of plants in the bamboo, Dendrocalamus strictus. Plant Cell Rep., 4: 191-194.
Sastri, B.N. (1952). The Wealth of India: Raw Materials, Vol. III: D-E. Council of Scientific and Industrial Research, New Delhi
Saxena, S. (1990). In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation. Plant Cell Rep., 9: 431-434.
Thorpe, T. A., Harry, I. S. and Kumar, O. P. (1991). Application of micropropagation to forestry. In: P. C. Debergh and R H Zimmerman (Eds.), Micropropagation: technology and application. Kluwer, Dordrecht.
Vatsala. (2003). Bamboos in India. NISCAIR, New Delhi.
Yashoda, R., Sumathi, R., Malliga, P. and Gurumurthi, G. (1997). Genetic enhancement and mass production of quality propagules of Bambusa nutans and Dendrocalamus membranaceus. Ind For., 4: 303-306.
Yeh, M. L. and Chang, W. C. (1986a). Plant regeneration through somatic embryogenesis in callus culture of green bamboo (Bambusa oldhamii). Theor Appl Genet., 73: 161-163.
Yeh, M. L. and Chang, W. C. (1986b). Somatic embryogenesis and subsequent plant regeneration from inflorescence callus of Bambusa beecheyana Munro var. beecheyana. Plant Cell Rep., 5: 409-411.
Zamora, A. B., Gruezo, S. S. and Damasco, O. P. 1989. Callus induction and plant regeneration from internode tissues of Dendrocalamus latiflorus cv. Machiko. In: A. N. Rao and A. M. Yusoff (Eds.), Proc. of the seminar on tissue culture of forest species. FRI, Malaysia and IDRC, Singapore.
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How to Cite
Micropropagation of Dendrocalamus strictus nees from mature nodal explants. (2012). Journal of Applied and Natural Science, 4(1), 5-9. https://doi.org/10.31018/jans.v4i1.213
