Exploration of multitrait antagonistic microbes against Fusarium oxysporum f.sp. lycopersici
Article Main
Abstract
Fusarium wilt is one of the major diseases of tomato causing extensive loss of production. Exploration of agriculturally important microbes (AIMs) for management of the tomato wilt is an ecofriendly and cost effective approach. In the present study, a total 30 Trichoderma and 30 bacterial isolates were screened in the laboratory for their biocontrol activity against Fusarium oxysporum f.sp. lycopersici (FOL). Out of all the isolates tested, Trichoderma asperellum BHU P-1 and Ochrobactrum sp. BHU PB-1 were found to show maximum inhibition of FOL in dual culture assay. Both the microbes also exhibited plant growth promoting activities such as phosphate solubilisation, production of siderophore, hydrogen cyanide (HCN), indole acetic acid (IAA) and protease activity. These microbes could be evaluated further in greenhouse and field studies for their potential use in management of Fusarium wilt of tomato.
Article Details
Article Details
Keywords
Fusarium oxysporum f.sp. lycopersici, Trichoderma, Ochrobactrum, Tomato
References
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Bakker, A.W., and Schipper, B. (1987). Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biology Biochemistry, 19, 451–457
Bashan, Y., andDe-Bashan, L. E. (2010). How the plant growth-promoting bacterium Azospirillum promotes plant growth—a critical assessment. Advances in agronomy (Vol. 108), Academic Press, pp. 77-136
Bisen, K., Keswani, C., Patel, J. S., Sarma, B. K., andSingh, H. B. (2016). Trichoderma spp.: efficient inducers of systemic resistance in plants. Microbial-mediated induced systemic resistance in plants. Springer, Singapore, pp 185-195.
Choudhary, D. K., Kasotia, A., Jain, S., Vaishnav, A., Kumari, S., Sharma, K. P., and Varma, A. (2016). Bacterial-mediated tolerance and resistance to plants under abiotic and biotic stresses. Journal of Plant Growth Regulation, 35(1), 276-300.
Datta, C., and Basu, P. (2000). lndole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub Cajanus cajan. Microbiological Research, 155, 123 - 127.
Elad, Y. (2000). Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Protection, 19(8-10), 709-714.
Felici, C., Vettori, L., Giraldi, E., Forino, L. M. C., Toffanin, A., Tagliasacchi, A. M., and Nuti, M. (2008). Single and co-inoculation of Bacillus subtilis and Azospirillum brasilense on Lycopersicon esculentum: effects on plant growth and rhizosphere microbial community. Applied Soil Ecology, 40(2), 260-270.
Forchetti, G., Masciarelli, O., Alemano, S., Alvarez, D., and Abdala, G. (2007). Endophytic bacteria in sunflower (Helianthus annuus L.): isolation, characterization, and production of jasmonates and abscisic acid in culture medium. Applied Microbiology and Biotechnology, 76(5), 1145-1152.
Gordon, S. A., and Weber, R. P. (1951). Colorimetric estimation of indole acetic acid. Plant Physiology, 26, 192–195. DOI: 10.1104/pp.26.1.192
Grondona, I., Hermosa, R., Tejada, M., Gomis, M.D., Mateos, P.F., Bridge, P.D., Monte, E. and Garcia-Acha, I. (1997). Physiological and biochemical characterization of Trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens. Applied and Environmental Microbiology, 63(8), 3189-3198.
Howell, C. R. (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Disease, 87(1), 4-10.
Jain, S., Vaishnav, A., Kasotia, A., Kumari, S., and Choudhary, D.K. (2014). Plant growth-promoting bacteria elicited induced systemic resistance and tolerance in plants. Emerging Technologies and Management of Crop Stress Tolerance Volume 2, Elsevier, pp 109-132 DOI: 10.1016/B978-0-12-800875-1.00005_3
Katan, J. (1971). Symptomless carriers of the tomato Fusarium wilt pathogen. Phytopathology, 61(10), 1213-1217.
King, J.E. (1932). The colorimetric determination of phosphorus. Biochemistry Journal, 26: 292.
Kumar, G., Maharshi, A., Patel, J., Mukherjee, A., Singh, H. B., and Sarma, B. K. (2017). Trichoderma: a potential fungal antagonist to control plant diseases. SATSA Mukhapatra-Annual Technical Issue, 21, 206-218.
Kumari, S., Vaishnav, A., Jain, S., Varma, A., and Choudhary, D. K. (2015). Bacterial-mediated induction of systemic tolerance to salinity with expression of stress alleviating enzymes in soybean (Glycine max L. Merrill). Journal of Plant Growth Regulation, 34(3), 558-573.
Liu, C. H., Chiu, C. S., Ho, P. L., and Wang, S. W. (2009). Improvement in the growth performance of white shrimp, Litopenaeus vannamei, by a protease?producing probiotic, Bacillus subtilis E20, from natto. Journal of Applied Microbiology, 107(3)1031-1041.
Loper, J. E., and Schroth, M. N. (1986). Influence of bacterial sources of indole-3-acetic acid on root elongation of sugar beet. Phytopathology, 76(4), 386-389.
Mehta, S., and Nautiyal, C. S. (2001). An efficient method for qualitative screening of phosphate-solubilizing bacteria. Current Microbiology, 43(1):51-56
Perrig, D., Boiero, M. L., Masciarelli, O. A., Penna, C., Ruiz, O. A., Cassán, F. D., and Luna, M. V. (2007). Plant-growth-promoting compounds produced by two agronomically important strains of Azospirillum brasilense, and implications for inoculant formulation. Applied Microbiology and Biotechnology, 75(5), 1143-1150.
Richardson, A. E., Barea, J. M., McNeill, A. M., and Prigent-Combaret, C. (2009). Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and Soil, 321(1-2), 305-339.
Rijavec, T., and Lapanje, A. (2016). Hydrogen cyanide in the rhizosphere: not suppressing plant pathogens, but rather regulating availability of phosphate. Frontiers in Microbiology, 7, 1785.
Schreinemachers, P., Simmons, E. B., and Wopereis, M. C. (2018). Tapping the economic and nutritional power of vegetables. Global Food Security, 16, 36-45.
Schwyn, B., and Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160: 47–56.
Sharma, A., and Johri, B. N. (2003). Growth promoting influence of siderophore-producing Pseudomonas strains GRP3A and PRS9 in maize (Zea mays L.) under iron limiting conditions. Microbiological Research, 158(3), 243-248.
Singh HB (2014a) Management of plant pathogens with microorganisms. Proceeding of National Academy of Science, 80: 443-454
Singh, H. B., Singh, A., Sarma, B. K., and Upadhyay, D. N. (2014b). Trichoderma viride 2% WP (Strain No. BHU-2953) formulation suppresses tomato wilt caused by Fusarium oxysporum f. sp. lycopersici and chilli damping-off caused by Pythium aphanidermatum effectively under different agroclimatic conditions. International Journal of Agriculture Environment and Biotechnology, 7, 313-320.
Stevens, C., Khan, V.A., Rodriguez-Kabana, R., Ploper, L.D., Backman, P.A., Collins, D.J., Brown, J.E., Wilson, M.A. and Igwegbe, E.C.K. (2003). Integration of soil solarization with chemical, biological and cultural control for the management of soilborne diseases of vegetables. Plant and Soil, 253(2):493-506
Swain, M. R., and Ray, R. C. (2009). Biocontrol and other beneficial activities of Bacillus subtilis isolated from cowdung microflora. Microbiological Research, 164(2), 121-130.
Vacheron, J., Desbrosses, G., Bouffaud, M.L., Touraine, B., Moënne-Loccoz, Y., Muller, D., Legendre, L., Wisniewski-Dyé, F. and Prigent-Combaret, C. (2013). Plant growth-promoting rhizobacteria and root system functioning. Frontiers in Plant Science, 4, 356.
Vaishnav, A., Jain, S., Kasotia, A., Kumari, S., Gaur, R. K., and Choudhary, D. K. (2014). Molecular mechanism of benign microbe-elicited alleviation of biotic and abiotic stresses for plants. Approaches to Plant Stress and their Management. Springer, New Delhi. pp. 281-295.
Vaishnav, A., Varma, A., Tuteja, N., and Choudhary, D. K. (2016). PGPR-mediated amelioration of crops under salt stress. Plant-Microbe Interaction: An Approach to Sustainable Agriculture. Springer, Singapore. pp. 205-226
Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Woo, S. L., and Lorito, M. (2008). Trichoderma–plant–pathogen interactions. Soil Biology and Biochemistry, 40(1), 1-10.
Wargovich MJ (2000) Anticancer properties of fruits and vegetables. Horticultural Science 35 (4): 573-575
Antoun, H., and Prévost, D. (2005). Ecology of plant growth promoting rhizobacteria. . PGPR: biocontrol and biofertilization. Springer, Dordrecht, 1–38.
Bakker, A.W., and Schipper, B. (1987). Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biology Biochemistry, 19, 451–457
Bashan, Y., andDe-Bashan, L. E. (2010). How the plant growth-promoting bacterium Azospirillum promotes plant growth—a critical assessment. Advances in agronomy (Vol. 108), Academic Press, pp. 77-136
Bisen, K., Keswani, C., Patel, J. S., Sarma, B. K., andSingh, H. B. (2016). Trichoderma spp.: efficient inducers of systemic resistance in plants. Microbial-mediated induced systemic resistance in plants. Springer, Singapore, pp 185-195.
Choudhary, D. K., Kasotia, A., Jain, S., Vaishnav, A., Kumari, S., Sharma, K. P., and Varma, A. (2016). Bacterial-mediated tolerance and resistance to plants under abiotic and biotic stresses. Journal of Plant Growth Regulation, 35(1), 276-300.
Datta, C., and Basu, P. (2000). lndole acetic acid production by a Rhizobium species from root nodules of a leguminous shrub Cajanus cajan. Microbiological Research, 155, 123 - 127.
Elad, Y. (2000). Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Protection, 19(8-10), 709-714.
Felici, C., Vettori, L., Giraldi, E., Forino, L. M. C., Toffanin, A., Tagliasacchi, A. M., and Nuti, M. (2008). Single and co-inoculation of Bacillus subtilis and Azospirillum brasilense on Lycopersicon esculentum: effects on plant growth and rhizosphere microbial community. Applied Soil Ecology, 40(2), 260-270.
Forchetti, G., Masciarelli, O., Alemano, S., Alvarez, D., and Abdala, G. (2007). Endophytic bacteria in sunflower (Helianthus annuus L.): isolation, characterization, and production of jasmonates and abscisic acid in culture medium. Applied Microbiology and Biotechnology, 76(5), 1145-1152.
Gordon, S. A., and Weber, R. P. (1951). Colorimetric estimation of indole acetic acid. Plant Physiology, 26, 192–195. DOI: 10.1104/pp.26.1.192
Grondona, I., Hermosa, R., Tejada, M., Gomis, M.D., Mateos, P.F., Bridge, P.D., Monte, E. and Garcia-Acha, I. (1997). Physiological and biochemical characterization of Trichoderma harzianum, a biological control agent against soilborne fungal plant pathogens. Applied and Environmental Microbiology, 63(8), 3189-3198.
Howell, C. R. (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Disease, 87(1), 4-10.
Jain, S., Vaishnav, A., Kasotia, A., Kumari, S., and Choudhary, D.K. (2014). Plant growth-promoting bacteria elicited induced systemic resistance and tolerance in plants. Emerging Technologies and Management of Crop Stress Tolerance Volume 2, Elsevier, pp 109-132 DOI: 10.1016/B978-0-12-800875-1.00005_3
Katan, J. (1971). Symptomless carriers of the tomato Fusarium wilt pathogen. Phytopathology, 61(10), 1213-1217.
King, J.E. (1932). The colorimetric determination of phosphorus. Biochemistry Journal, 26: 292.
Kumar, G., Maharshi, A., Patel, J., Mukherjee, A., Singh, H. B., and Sarma, B. K. (2017). Trichoderma: a potential fungal antagonist to control plant diseases. SATSA Mukhapatra-Annual Technical Issue, 21, 206-218.
Kumari, S., Vaishnav, A., Jain, S., Varma, A., and Choudhary, D. K. (2015). Bacterial-mediated induction of systemic tolerance to salinity with expression of stress alleviating enzymes in soybean (Glycine max L. Merrill). Journal of Plant Growth Regulation, 34(3), 558-573.
Liu, C. H., Chiu, C. S., Ho, P. L., and Wang, S. W. (2009). Improvement in the growth performance of white shrimp, Litopenaeus vannamei, by a protease?producing probiotic, Bacillus subtilis E20, from natto. Journal of Applied Microbiology, 107(3)1031-1041.
Loper, J. E., and Schroth, M. N. (1986). Influence of bacterial sources of indole-3-acetic acid on root elongation of sugar beet. Phytopathology, 76(4), 386-389.
Mehta, S., and Nautiyal, C. S. (2001). An efficient method for qualitative screening of phosphate-solubilizing bacteria. Current Microbiology, 43(1):51-56
Perrig, D., Boiero, M. L., Masciarelli, O. A., Penna, C., Ruiz, O. A., Cassán, F. D., and Luna, M. V. (2007). Plant-growth-promoting compounds produced by two agronomically important strains of Azospirillum brasilense, and implications for inoculant formulation. Applied Microbiology and Biotechnology, 75(5), 1143-1150.
Richardson, A. E., Barea, J. M., McNeill, A. M., and Prigent-Combaret, C. (2009). Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and Soil, 321(1-2), 305-339.
Rijavec, T., and Lapanje, A. (2016). Hydrogen cyanide in the rhizosphere: not suppressing plant pathogens, but rather regulating availability of phosphate. Frontiers in Microbiology, 7, 1785.
Schreinemachers, P., Simmons, E. B., and Wopereis, M. C. (2018). Tapping the economic and nutritional power of vegetables. Global Food Security, 16, 36-45.
Schwyn, B., and Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry, 160: 47–56.
Sharma, A., and Johri, B. N. (2003). Growth promoting influence of siderophore-producing Pseudomonas strains GRP3A and PRS9 in maize (Zea mays L.) under iron limiting conditions. Microbiological Research, 158(3), 243-248.
Singh HB (2014a) Management of plant pathogens with microorganisms. Proceeding of National Academy of Science, 80: 443-454
Singh, H. B., Singh, A., Sarma, B. K., and Upadhyay, D. N. (2014b). Trichoderma viride 2% WP (Strain No. BHU-2953) formulation suppresses tomato wilt caused by Fusarium oxysporum f. sp. lycopersici and chilli damping-off caused by Pythium aphanidermatum effectively under different agroclimatic conditions. International Journal of Agriculture Environment and Biotechnology, 7, 313-320.
Stevens, C., Khan, V.A., Rodriguez-Kabana, R., Ploper, L.D., Backman, P.A., Collins, D.J., Brown, J.E., Wilson, M.A. and Igwegbe, E.C.K. (2003). Integration of soil solarization with chemical, biological and cultural control for the management of soilborne diseases of vegetables. Plant and Soil, 253(2):493-506
Swain, M. R., and Ray, R. C. (2009). Biocontrol and other beneficial activities of Bacillus subtilis isolated from cowdung microflora. Microbiological Research, 164(2), 121-130.
Vacheron, J., Desbrosses, G., Bouffaud, M.L., Touraine, B., Moënne-Loccoz, Y., Muller, D., Legendre, L., Wisniewski-Dyé, F. and Prigent-Combaret, C. (2013). Plant growth-promoting rhizobacteria and root system functioning. Frontiers in Plant Science, 4, 356.
Vaishnav, A., Jain, S., Kasotia, A., Kumari, S., Gaur, R. K., and Choudhary, D. K. (2014). Molecular mechanism of benign microbe-elicited alleviation of biotic and abiotic stresses for plants. Approaches to Plant Stress and their Management. Springer, New Delhi. pp. 281-295.
Vaishnav, A., Varma, A., Tuteja, N., and Choudhary, D. K. (2016). PGPR-mediated amelioration of crops under salt stress. Plant-Microbe Interaction: An Approach to Sustainable Agriculture. Springer, Singapore. pp. 205-226
Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Woo, S. L., and Lorito, M. (2008). Trichoderma–plant–pathogen interactions. Soil Biology and Biochemistry, 40(1), 1-10.
Wargovich MJ (2000) Anticancer properties of fruits and vegetables. Horticultural Science 35 (4): 573-575
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Exploration of multitrait antagonistic microbes against Fusarium oxysporum f.sp. lycopersici. (2019). Journal of Applied and Natural Science, 11(2), 503-510. https://doi.org/10.31018/jans.v11i2.2111
