V. Sridevi M. Raghuram


Sugar industrial effluents possess high amounts of toxic pollutants and contaminate the receiving sites. Treatment of contaminated sites by using microorganisms provides an alternate to conventional methods hence demands in the identification of metal tolerant microorganisms has been increasing day by day. Therefore in this study soil samples collected from Tanuku sugar factory residual effluent point (bank of Gosthani river), west Godavari district A.P were analyzed for the bacterial tolerance to Copper (Cu), Zinc (Zn) and Lead (Pb) in their chloride forms. Additionally, the study was carried out to identify the metal tolerant bacteria by morphological, biochemical and 16S rRNA gene sequencing studies. Four potential bacterial isolates were selected to analyze metal tolerance against CuCl2, ZnCl2, and PbCl2. The sequences were compared with those in NCBI and submitted in gene bank with accession numbers MK100333 (Paenibacillus cookie), MK100334 (Bacillus cereus), MK100335 (Aneurini bacillus sp) and MK100387 (Paenibacillus sp.). A Phylogenetic tree was constructed to Paenibacillus sp. the highly efficient bacterial strain among the four isolates using MEGA 7 soft ware. The results of this study showed that P. dentritiformis had multiple metal tolerances (Cu, Zn and Pb) up to 500mg/L after 72 hrs.  The identified bacterial strain proved to be the strong heavy metal tolerant bacterial strain. Hence, its usage will be helpful in the treatment of heavy metals specifically Cu, Zn and Pb contaminated soils and further optimization of these cultures is required to improve its metal resistant capacity.




Copper, Lead removal, Metal tolerance, Paenibacillus species, Zinc removal, 16s rRNA gene sequencing

Hookoom M, Puchooa D (2013) Isolation and identification of heavy metals tolerant bacteria from industrial and agricultural areas in Mauritius. Curr Res Microbiol Biotechnol., 1:119–123
Smrithi A, Usha K (2012) Isolation and characterization of chromium removing bacteria from tannery effluent disposal site. Int. J. Adv. Biotechnol. Res., 3:644–652.Open Access.
Shuttleworth KL, Unz RF (1993) Sorption of heavy metals to the filamentous bacterium Thiothrix strain A1. Appl. Environ. Microbiol., 59:1274–1282.
Salinas E, Melorza DO (2000) Removal of cadmium and lead from dilute aqueous solution by Rhodotorularuba. Bioresour Technol., 72:107–112.
Anoop K, Viraraghvan T (1999) Removal of heavy metals using the fungus Aspergillus niger. Bioresour Technol., 70:95–104. https://doi.org/10.1016/s0960-8524(98)00192-8.
Ahuja P, Gupta R (1999) Zn+2 biosorption by Oscillatoria angustissima. Process Biochem., 34:77–85.
Yasar A, Khan M, Tabinda AB, Hayyat MU, Zaheer A (2013) Percentage uptake of heavy metals of different macrophytes in stagnant and flowing textile effluent. J Anim Plant Sci.,23:1709–1713.
V. Sridevi, M.Raghuram and D. Ravisankar (2018) Isolation and Screening of Heavy Metal Resistant Microorganisms from Industrial Soil. Journal of Pure and Applied Microbiology, Sept. 2018. Vol. 12(3): 1667-1674. http://dx.doi.org/10.22207/JPAM.12.3.75.
F. Abraham Samuel, V. Mohan and L. Jeyanthi Rebecca (2014) Physicochemical and heavy metal analysis of sugar mill effluent. J. Chem. Pharm. Res., 2014, 6(4):585-58. Open Access.
Suresh B, Sudhakar. G, Damodharam T.(2015). Determination of Heavy Metals in Sugar Industry Effluent, International Journal of Modern Engineering Research (IJMER), Vol. 5, Iss.4. Open Access.
Usha Damodhar and M. Vikram Reddy (2012). Assessment of trace metal pollution of water and sediment of river Gadilam (Cuddalore, South east coast of India) receiving sugar industry effluents. Continental J. Environmental Sciences, 6 (3): 8 – 24
R. A. Ansari, A. A. Qureshi & D. S. Ramteke (2016). Isolation and characterization of heavy-metal resistant microbes from Industrial soil. International Journal Of Environmental Sciences, Volume 6, No5, 2016. doi: 10.6088/ijes.6063.
Seralathan Kamala-Kannan and Kui Jae Lee (2008). Metal Tolerance and Antibiotic Resistance of Bacillus species Isolated from Sunchon Bay Sediments, South Korea, 7 (1): 149-152. DOI: 10.3923/biotech.2008.149.152.
Shameer Syed  and Paramageetham Chinthala (2015). Heavy Metal Detoxification by Different Bacillus Species Isolated from Solar Salterns. http://dx.doi.org/10.1155/2015/319760
Shruti Murthy, Geetha balli and S.K. Sarangi (2013). Effect of lead on growth, protein and biosorption capacity of Bacillus cereus isolated from industrial effluents. A Journal of Environmental Biology.
Y?lmaz IE (2003) Metal tolerance and biosorption capacity of Bacillus circulans strain EB1. Res Microbiol.,154:409–415. https://doi.org/10.1016/S0923-2508(03)00116-5.
Reddy CA, Beveridge TJ, Breznak JA, Marzluf G (2007). Methods for general and molecular microbiology. (3rdedn), ASM Press, ASM, Washington DC.
Shruti Murthy, Geetha Bali and S. K. Sarangi (2012). Biosorption of Lead by Bacillus cereus Isolated from Industrial Effluents. British Biotechnology Journal, 2(2): 73-84, 2012.
M.M. Areco, S. Hanela, J. Duran, M.D.S.(2012). Afonso Biosorption of Cu(II), Zn(II), Cd(II) and Pb(II) by dead biomasses of green alga Ulva lactuca and the development of a sustainable matrix for adsorption implementation. J Hazard Mater, 213–4 , 123-132. https://doi.org/10.1016/j.jhazmat.2012.01.073.
Chang, J.S., Law, R., Chang, C. (1997). Biosorption of lead, copper and cadmium by biomass of Pseudomonas aeruginosa PU 21. Water Research, 31: 1651-1658. http://dx.doi.org/10.1016/S0043-1354(97)00008-0.
M. Govarthanan, R. Mythili, T. Selvankumar, S. Kamala-Kannan, A. Rajasekar and Young-Cheol Chang (2016). Bioremediation of heavy metals using an endophytic bacterium Paenibacillus sp. RM isolated from the roots of Tridaxprocumbens. Biotech. 6:242. doi: 10.1007/s13205-016-0560-1.
D.H. Bergey, R.E. Buchanan, N.E. Gibbons (1974). Bergey's Manual of Determinative Bacteriology (eighth ed.), Williams and Wilkins Co., Baltimore,pp 1246
A. Hamid, A. Mushtaq, R. Nazir and S. Asghar (2017). Heavy metals in soil and vegetables grown with municipal wastewater in Lahore. Bangladesh. J. Sci. Ind. Res., 52(4), 331-336. DOI: https://doi.org/10.3329/bjsir.v52i4.34821.
XiaoX., Luo S.L., Zeng G.M, Wei W.Z., Wan Y., Chen L., Guo H.J., Cao Z., Yang L.X., Chen J.L., Xi Q., 2010. Bioabsorption of cadmium by endophyticus fungus (EF) Microsphaeropsis sp. LSE10 isolated from cadmium hyperaccumulator Solanum nigrum L. Bioresour. Technol., 101, 1668-1674 doi: 10.1016/j.biortech.2009.09.083.
Zouboulis, A.I., Loukidou, M.X., Martis, K.A., 2004. Biosorption of toxic metals from aqueous solutions by bacteria strains isolated from metal polluted soils. Process Biochem., 39 909-916. DOI: 10.1016/s0032-9592(03)00200-0
Leedjarv, A., Ivask, A., Virta, M., 2008. The interplay of different transporters in the mediation of divalent heavy metal resistance in Pseudomonas putida KT2440. J.Bacteriol., 190 2680-2689. doi: 10.1128/JB.01494-07
M. Govarthanan, R. Mythili, T. Selvankumar, S. Kamala-Kannan, A. Rajasekar and Young-Cheol Chang (2016). Bioremediation of heavy metals using an endophytic bacterium Paenibacillus sp. RM isolated from the roots of Tridaxprocumbens. Biotech., 6:242. DOI 10.1007/s13205-016-0560-1.
Hao, O.J., Huang, L., Chen, J.M., Buglass, R.L. (1994). E?ects of metal additions on sulfate reduction activity in wastewaters. Toxicological and Environmental Chemistry, 46, 197–212. https://doi.org/10.1080/02772249409358113.
Mohammed Umar Mustapha and NormalaHalimoon. (2015). Screening and isolation of heavy metal tolerant bacteria in industrial effluent. Procedia Environmental Sciences, 30: 33 – 37. https://doi.org/10.1016/j.proenv.2015.10.006.
Saitou N. and Nei M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4:406-425. DOI: 10.1093/oxfordjournals.molbev.a040454.
Tamura K., Nei M., and Kumar S. (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA), 101:11030-11035. DOI: 10.1073/pnas.0404206101.
Kumar S., Stecher G., and Tamura K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33:1870-1874. doi: 10.1093/molbev/msw054.
Research Articles

How to Cite

Multiple metal tolerance of Paenibacillus dentritiformis isolated from metal contaminated soils west Godavari district (Andhra Pradesh): MUTLTIPLE METAL TOLERANCE OF PAENIBACILLUS DENTRITIFORMIS. (2019). Journal of Applied and Natural Science, 11(2), 486-491. https://doi.org/10.31018/jans.v11i2.2094