Preeti G . Dharmik Ashok V. Gomashe


The study was carried out from July 2010 to June 2011. The aim of the present investigation was to isolate Staphylococcus aureus from raw milk obtained from cow from different parts of Nagpur district, India and to determine their antibiotic susceptibility profile. General biochemical tests along with the MASTASTAPHTM rapid agglutination tests were employed for bacterial identification. All the well characterized isolates were further confirmed by amplifying coa gene by PCR. A total 95 S. aureus isolates were obtained during this study from 50 raw milk samples. When all the well characterized isolates were subjected to antibiotic susceptibility testing, erythromycin and chloramphenicol were the most effective antibiotics. A large proportion of isolates were found to be resistant to Co-trimoxazole (89%) followed by Linomycin (81%) and Penicillin (35%) It was concluded from the current study that the hygiene of the milk was poor and resistant strains contaminated the milk probably during the process of transportation.


Download data is not yet available.


Metrics Loading ...




Antibiotic susceptibility, Staphylococcus aureus, Raw milk

Astal, Z., EI-manama, A. and Sharif, F.A. (2002). Antibiotic resistance of bacteria associated with community acquired urinary tract infection in the southern area of Gaza Strip. J Chemother, 14 (3):259-64.
Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Truck, M. (1966). Antibiotic Susceptibility testing by standardized single disc method. Am. J. Clin. Pathol., 45: 493-496.
Braddy, A.J. (2002). Bovine mastitis an evolving disease. Vet J. 164 : 116-128.
Chigbu, C.O. and Ezeronye, O.U. (2003). Antibiotic Staphylococcus in Abia State, Nigeria. African J Biotech, 2 (10) : 374-378.
Collins, N., Ateba, Moses Mbewe, Modisane, S., Moneoang, Cornelius, C. and Bezuidenhout (2010). Antibiotic resistant S. aureus isolated from milk in the Mafikeng area, North West province, South Africa. S. Afr. J. Sci., 106 : 11 - 12.
Devriese, L.A., Haesebrouck, F., Hommez, H. and Vandermeersch, R. (1997). A 25-year survey of antibiotic susceptibility testing in Staphylococcus aureus from bovine mastitis in Belgium, with special reference to penicillinase. Vlaams Diergeneeskundig Tijdschrift, 66 : 170-173.
Esron, D., Karimuebo, E., Lughano, T., Kusilika, R. H., Melegela, Aglowisye, M., Kappa, M. and Kalvin, S. (2005). Study on mastitis, milk quality and health risk associated with consumption of milk from pastoral herds in Dodoma and Morgora region, Tanzania. Vet. Sci., 6 : 213 - 221.
Evenson, M.L., Hinds, M.W., Bernstein, R.S. and Bergdoll, M.S. (1988). Estimation of human dose of staphylococcal enterotoxin A from large outbreak of staphylococcal food poisoning involving chocolate milk. Int J Food Microbiol, 7:311–316.
Farzana, K., Nisar, S., Shah, H. and Jabee, F. (2004). Antibiotic resistance pattern against various isolates of Staphylococcus aureus from raw milk samples. J Research Science, 15 (2): 145 - 151.
Garrod, L.P., Lambert, H.P. and Grady, F.O. (1981). In: Antibiotic Chemotherapy, 5th ed., Churchill, Livingstone, Edinburgh.
Hassan, Z.A., Shaw, E.J. and Shooter, R.A. (1978). Changes in antibiotic sensitivity in strains of Staphylococcus aureus. Brit. Med. J., 2 : 536.
Larsen, H.D., Sloth, K.H. and Elsberg, C. (2000). The dynamics of Staphylococcus aureus intramammary infections in nine Danish dairy herds. Vet Microbio., 71 : 89-101.
Mahon, C.R and Larsen, H.S. (1995). Staphylococcus aureus. In: Mahon CR, Manuseseilis G Jr., editors. Textbook of diagnostic microbiology. New York: WB Saunders Company, pp. 325-330.
Malahat Ahmadi, Sayad Mehdi Razavi Rohani, Nooshin Ayremloun (2010). Detection of Staphylococcus aureus in milk by PCR.Comp Clin Pathol, 19: 91-94.
Matsunaga, T., Kamata, S., Kakiichi, N. and Uchida K. (1993). Characteristics of Staphylococcus aureus isolates from peracute, acute and chronic bovine mastitis. J Vet Med Sci., 55 : 297-300.
NCCLS, National Committee for Clinical Laboratory Standards (1999). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Approved standard M13-A. Wayne: NCCL.
Odongo, O. and Ambani, A. (1989). Microorganisms isolated from bovine milk samples submitted to veterinary diagnostic laboratory, Kabete, Kenya. Bull Anim Health Prod Afr., 37: 195-196.
Onasanya, A., Mignouna, H.D. and Thottappilly, G. (2003). Genetic fingerprinting and phylogenetic diversity of Staphylococcus aureus isolates from Nigeria. African J. Biotech, 2(8):246-250.
Pitkala, A., Haveri, M., Pyorala, S., Myllys, V. and Honkanen- Buzalski, T. (2004). Bovine mastitis in Finlaand– Prevalence, distribution of bacteria and antimicrobial resisatnce. J Dairy Sci., 87: 2433-2441.
Sears, P.M. and McCarthy, K.K. (2003). Management and treatment of Staphylococcal mastitis. Vet Ciin North Am Food Anim Pract., 19:171-185.
Shoemaker, E.H. and Yow, E.M. (1954).Clinical evaluation of erythromycin. Arch. Intern. Med., 3: 397-401.
Uwaezuoke, J.C., Aririatu, LE. (2004). A servey of Antibiotic resistant Staphylococcus aureus strains from clinical sources in Owerri. J Appl Sci Environ Managt., 8(1):67-8.
Citation Format
How to Cite
Dharmik, P. G. ., & Gomashe, A. V. (2011). Isolation, characterization and antibiotic susceptibility profile of Staphylococcus aureus from raw milk samples in Nagpur district, India. Journal of Applied and Natural Science, 3(2), 319-322. https://doi.org/10.31018/jans.v3i2.208
More Citation Formats:
Research Articles