Fractal analysis of colony margins as an aid for screening freshwater yeast cultures for bioclarification of turbid polluted water resources
Article Main
Abstract
In Iron ore mining areas of Goa, water resources are polluted due to high turbidity and mineral colloids. For bioclarification of the turbidity, we need to identify some promising property of strains by which the strains of freshwater yeasts can be screened. This work presents a screening of freshwater yeast cultures, based on the complexity of colony margins. We performed screening of the wild aquatic yeasts isolated from different fresh water bodies of Goa on 2nd, 4th and 6th day of incubation respectively. Colony margins of sixteen different strains were studied for their fractality indexes and on comparison significant differences were observed among them. We report comparative analysis of five representative strains in this paper. Particularly strain Bchlm-1-2 showed high fractality index approximately 1410 on 6th day of incubation. This work provides quantitative scoring system of the morphological behavior of large number of strains. Our approach has the potential to improve the accuracy and speed to quantify and compare large number of isolates on the basis of their colony margins.
Article Details
Article Details
Keywords
Biosedimentation, Colony margins, Flocculation, Fractal dimensions
References
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Barry, D., and Mcgee, S. (2009). Relating Fractal Dimension to Branching Behaviour in Filamentous Microorganisms, ISAST Transactions on Electronics and Signal Processing, 4 (1): 71-76.
Bellouti, M. (1997) Flocs vs granules : differentiation by fractal dimension. Water Research, 31:5 1227-1231
Boddy L, Wells J. M., Culshaw C, Donnelly D.P. (1999). Fractal analysis in studies of mycelium in soil. Geoderma, 88:301-328.
Gadd, G. M., Ramsay, L., Crawford, J. W. and Ritz, K. (2001). Nutritional influence on fungal colony growth and biomass distribution in response to toxic metals. FEMS Microbiology Letters, 204(2), 311-316.
Gonzalez-perez, A., Feld, K., and Ruso, J. M. (2016). Polymersomes mimic biofilms fractal growth. Journal of Polymer Research, 1–6.
Gontar A, Bottema M.J., Binder, B.J., Tronnolone, H. (2018). Characterizing the shape patterns of dimorphic yeast pseudohyphae. R. Soc. Open Sci., 5: 180820
Ji, Z., Card, K.J. and Dazzo, F.B. (2015) CMEIAS J Frad: A digital computing tool to discriminate the Fractal geometry of landscape architectures and spatial patterns of individual cells in microbial biofilms, Microb Ecol., 69:710-720
Lestari, V. D., Mardawati, E., and Nurhadi, B. (2018). Fractal Dimension Analysis of Texture Formation of Whey Protein-Based Foods, International Journal of Food Science Volume 2018, Article ID 7673259,1- 17
Lee, C., and Kramer, T. A. (2004). Prediction of three-dimensional fractal dimensions using the two-dimensional properties of fractal aggregates, Advances in colloid and Interface Science, 112, 49–57.
Li, D. H., and Ganczarczyk, J. (1989). Fractal geometry of particle aggregates generated in water and wastewater treatment processes. Environmental Science and Technology, 23(11), 1385-1389.
Li, T. Z. Zhu, D.S. (2006) Wang, C.H. Yao, H.X. Tang, Characterization of floc size, strength and structure under various coagulation mechanisms, Powder Technol., 168 104–110.
Li, X. Y., and Leung, R. P. (2005). Determination of the fractal dimension of microbial flocs from the change in their size distribution after breakage. Environmental Science and Technology, 39(8), 2731-2735.
Logan, B. E. (1991.). Fractal Coagulation Kinetics. Department of Civil and Environmental Engineering The Pennsylvania State University.
Luan, L., Sun, Y., Chen, S., Wu, C., and Hu, Y. (2018). A study of fractal dimension as a quality indicator of hairtail ( Trichiurus haumela ) samples during frozen storage. Scientific Reports, (December 2017), 1–8.
Lundy, S. D., Payne, R. J., Giles, K. R., and Garrill, A. (2001). Heavy metals have different effects on mycelial morphology of Achlya bisexualis as determined by fractal geometry, FEMS Microbiology Letters, 201 201, 259–263.
Mahallati, S. Z., Pazira, E., Abbasi, F., and Babazadeh, H. (2018). Estimation of soil water retention curve using fractal dimension. Journal of Applied Sciences and Environmental Management, 22(2), 173-178.
Malekani, K., Rice, J. A., and Lin, J. (1996). Comparison Of Techniques For Determining The F ractal Dimensions of clay mineral, Journal of Clays and Clay Minerals, 44(5), 677–685.
Obert, M., Pfeifer, P., Sernetz, M., and Giessen, J. (1990). Microbial Growth Patterns Described by Fractal Geometry, Journal of Bacteriology, 172(3), 1180–1185.
Obert. M. (1993). Microbial growth patterns: fractal and kinetic characteristics of patterns generated by a computer model to simulate fungal growth. Journal of Fractal Biology, 254-374.
Papagianni, M. (2006). Quantification of the fractal nature of mycelial aggregation in Aspergillus niger submerged cultures, Microbial Cell Factories,
13:1–13
Papagianni M: (2004). Fungal morphology and metabolite production in submerged mycelial processes. Biotechno.l Adv. 22:189-259.
Prado, E. G. De, Rivas, E., Silóniz, M. De, Diezma, B., and Barreiro, P. (2014). Quantitative analysis of morphological changes in yeast colonies growing on solid medium?: the eccentricity and Fourier indices, Yeast 2014; 31: 431–440.
Puchkov, E. (2016). Image Analysis in Microbiology?: A review. Journal of Computer and Communications, 4 : 8-32.
Ravindranath S., Kamath, Bungay H,.(1998). Yeast colonies on solid media, Journal of General Microbiology (1988), 134, 3061-3069.
Ruusuvuori, P., Nykter, M., Yli-harja, O., Shmulevich, I., and Dudley, A. M. (2014). Quantitative analysis of colony morphology in yeast. Biotechniques. 2014 January ; 56(1): 18–27 56(1).
Saiedi, N.N., A. A. Besalatpour, H. Shirani, P. Abbaszadeh Dehaji, I. Esfandiarpour, M. Faramarzi (2017). Agregation and fractal dimension of aggregates formed in sand dunes stabilized by Pistachio PAM and PistachioPVAc, European Journal of Soil Science, 783–791.
Shirali, S. A. (2014). Fractal dimension and the cantor set, Resonance 1000–1004.
Sun, J., Qin, L., Li, G., and Kang, Y. (2013). Effect of hydraulic conditions on flocculation performances and floc characteristics in Chinese herbal extracts by chitosan and chitosan hydrochloride. Chemical Engineering Journal, 225: 641–649.
Tomankova, K. (2006). Use of image analysis to study growth and division of yeast cells, ImagingSci.Technol.50 : 583-589
Vesela, M., Zemskal, O., Vesely, M., Nezadal, M. (2001). The fractal analysis of image structures for bilogic applications, Digital Imagine in Biology and Medicine, 53-54.
Woriax, H. E. (2009). A study on fractal morphogenesis in bacteria as a response to environmental stress Hannah E . Woriax Honors College Thesis UNC Pembroke, 1–18.
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How to Cite
Fractal analysis of colony margins as an aid for screening freshwater yeast cultures for bioclarification of turbid polluted water resources. (2019). Journal of Applied and Natural Science, 11(2), 250-256. https://doi.org/10.31018/jans.v11i2.2028
