The aim of the present study was to isolate and identify azo dye degrading microbes using 16s r RNA sequencing. For this, ten effluent and fifty soil samples were collected from textile industry from a discharge panel of textile industries near Peenya and Magadi road Industrial area, Bengaluru, and dying industries near Kerur, Bagalkot, Karnataka, India. The pH, temperature, BOD, COD, Odor, Total dissolved solid (TDS), Total suspended solids (TSS), Chemical oxygen demand (COD), Biological oxygen demand (BOD), Dissolved Oxygen (DO), and Total Hardness values were very high when compared with the values given by the Bureau of Indian Standards. Pure cultures were screened on the basis of colony morphology. Three different types of unique cultures were selected and named as isolates S1, S2 and S3. Outof 6 dyes viz. Corafast blue, Corafast red, Red 3BN, Solophenyl Brown, RemazoleRG , Rubin M2B used, isolate S1 showed degradation on the maximum number of dyes Red 3BN, Corafast blue, Corafast red, in comparison to other isolates (isolates S2 and S3). Thus, isolate S1 was used for the further studies. For this study Red 3BN dye was chosen. The isolated bacterium was gram positive Bacilli. In the biochemical characterization, the isolate was partially confirmed as Bacillus sp.
Further, the selected isolate was identified by sequencing the 16S rRNA sequencing. The bacterial isolate was identified as Bacillus species exhibiting 99% similarity, the phylogenic relationship of 16S ribosomal RNA gene, partial sequence of the isolated strain and species related to Bacillus species in the gene bank database. Thus, this organism may be used significantly in effluent treatment such as textile, paper, ink and other industries.
Azo dye, Bacillus species, Degradation, Effluent, Fermentation, Pure culture
AmminiParvathi, Kiran Krishna, JiyaJose, Neetha Joseph. And SanthaNair.(2009). Biochemical and Molecular characterization of Bacillus pumilis isolated from coastal environment in Cochin, India.Brazilian Journal of Microbiology (40):269-275.
Andre, B.D.,Fransisco, J.C. and Jules,B.L. (2007). Review paper on current technologies for decolourization of textile effluent. Colourage,(40): 35-38.
Aneja, K.R. (2004). Experiments in Microbiology. New Age International (P) Limited Publishers,(4).
Azhar A. Hussain, Mohamed S. Abdel-Salam, Hoda H. Abo-Ghalia, Wafaa K. Hegazy, Safa S. Hafez (2017). Optimization and molecular identification of novel cellulose degrading bacteria isolated from Egyptian environment. Journal of Genetic Engineering and Biotechnology 15: 77–85.
Beuving, J., Christina, F.M., van der Donk., Catharina, F.M., Linssen Petra. F.G. Wolffs. andAnneliesVerbon. (2011).Evaluation of direct inoculation of the BD PHOENIX system from positive BACTEC blood cultures for both Gram-positive cocci and Gram-negative rods. BMC Microbiology, 11:156.
Gogate.P.R., and Pandit.A.B., (2004). A review of imperative technologies for wastewater hybrid methods. Adv.Environ.Res., 8: 553-597.
Hauwa Tahir, ZuwairaDanyaro, Halima Ladan, Sanusi Magaji (2016). Isolation and identification of Bacteria Involved in the Contamination of Some selected Fast foods Solid Within Wutin dada area, Bauchi. International Journal of Advances in Science Engineering and Technology, 4, (4).
Holding, A. J. and Collee, J. G. (1971). Chapter I Routine biochemical tests. In Methods in microbiology (Vol. 6, pp. 1-32). Academic Press.
Isabel Sa´nchez, Llanos Palop, and Cristina Ballesteros. (2000). Biochemical characterization of lactic acid bacteria isolated from spontaneous fermentation of ‘Almagro’ eggplants. International Journal of Food Microbiology, 59: 9–17
Laowansiri, Sunantha, SoydoaVinitnantharat, PawineeChaiprasert, and SungRyongHa. (2008). Anaerobic degradation kinetics of reactive dye with differentcarbon sources. J. Environ. Biol., (29): 309-314.
Manikandan, N., SurumbarKuzhali, S. and Kumuthakalavalli, R. (2012).Decolorisation of textile dye effluent using fungal microflora isolated from spent mushroom substrate (SMS). J. Microbiol. Biotech. Res, 2 (1): 57-62.
Mathur, Nupur.andPradeepBhatnagar.(2007). Mutagenicity assessment of textiledyes from Sanganer (Rajasthan). J. Environ. Biol., (28): 123-126.
Nigam, P. Armour, Banat. G., Singh, I.M., Marchant, D., McHale, R., McMullan, G. (2000) Physical removal of textile dyes from effluents and solid-state fermentation of dye-adsorbed agricultural residues. BioresTechnol., (74):179–179.
Pant Deepak, Anoop Singh, YaminiSatyawali, and Gupta, R.K. (2008). Effect of carbon and nitrogen source amendment on synthetic dyes decolourizing efficiency of white-rot fungus, Phanerochaete chrysosporium. J.Environ. Biol., (29): 79-84.
Rajeswari, K., Subashkumar, R. and Vijayaraman, K.J. (2013). Physico chemical parameters of Effluents collected from Tirupur Textile dyeing and CETP and analysis of Heterotropic bacterial population. Journal of Microbiology and Biotechnology Research, 3(5): 37–41
Robinson, T., McMullan, G.andNigam, P. (2001).Remediation of dyes in textile effluent: a critical review on current treatment technologies with a proposed alternative.Bioresource Technology, (77): 247-255.
Sofia Nosheen, Haq Nawaz and Khalil-UR-Rehman, (2000). Physico-Chemical Characterization of Effluents of Local Textile Industries of Faisalabad–Pakistan. International Journal of Agriculture and Biology, 2(3): 232-233.
Sriram, N. and Reetha, D.(2015). Isolation and characterization of dye degrading bacteria e effluents. Central European Journal of Experimental Biology, 4 (2):5-10.
Stolz, A. (2001). Basic and applied aspects in the microbial degradation of azo dyes. Appl. Microbiol. Biotechnol., (56): 69–80.
Sudha, M., Saranya, A., Selvakumar, G. and Sivakumar N.(2014).Microbial degradation of Azo Dyes: A review Int.J.Curr.Microbiol.App.Sci, 3(2): 670-690.
Sujatha, P., Naresh Kumar, B.andKalarani, V. (2012).Isolation, characterization and molecular identification of bacteria from tannery effluent using 16S rRNA sequencing. Current Biotica, 6 (2): 198-207.
ThokerFarook Ahmed, Manderia Sushi, and Manderia Krishna. (2012). Impact of Dye Industrial Effluent on Physicochemical Characteristics of Kshipra River, Ujjain City, India International Research Journal of Environment Sciences, 1(2): 41-45.
Trupti D. Chaudhari, Susan Eapen and Fulekar1, M. H. (2009). Characterization of industrial waste and identification of potential micro-organism degrading tributyl phosphate Journal of Toxicology and Environmental Health Sciences 1(1): 001-007.
Van Veen, S. Q.,sClaas, E. C. and EdKuijper, J. (2010).High-Through put Identification of Bacteria and Yeast by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in Conventional Medical Microbiology Laboratories. Journal of Clinical Microbiology, 3(48): 900–907.
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