Evaluation of antioxidant potential of alcoholic stem bark extracts of Bauhinia variegata Linn.

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Published Mar 10, 2019
DOI: https://doi.org/10.31018/jans.v11i1.2015

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Ashwani Kumar
Institute of Pharmacy, Shri Jagdish Prasad Jhabarmal Tibrewala University, Jhunjhunu - 333001 (Rajasthan), India
Vikas Anand
School of Pharmaceutical Sciences and Applied Chemistry, Sardar Bhagwan Singh University, Balawala, Dehradun-248161 (Uttarakhand), India
R. C. Dubey
Department of Botany and Microbiology, Gurukul Kangri Vishwavidyalaya, Haridwar-249407 (Uttarakhand), India
Kapil K. Goel
Department of Pharmaceutical Sciences, Gurukul Kangri Vishwavidyalaya, Haridwar-249407 (Uttarakhand), India

Abstract

The present study was carried out for the evaluation of in-vitro antioxidant potential of alcoholic stem bark (ALSB) extract of Bauhinia variegata Linn. Dried stem bark extract of B. variegata Linn. was screened to evaluate its free radical scavenging effect. Various methods like DPPH assay, reducing power assay, percentage scavenging activity of hydrogen peroxide and superoxide radical scavenging activity were used for screening in-vitro antioxidant potential. Antioxidant potentials were concentration dependent which were compared with standard antioxidants such as butylated hydroxyanisole (BHA) and ascorbic acid. The maximum scavenging effect of B. variegata Linn. alcoholic stem bark extract on  DPPH free radical, superoxide radical and hydrogen peroxide was 72.19 + 0.20, 81.60 + 0.22 and 76.06 + 0.16 respectively at a concentration of 2500 ?g/mL.  It was clearly indicated that the alcoholic extract of the stem bark has significant in vitro antioxidant activity. Currently available synthetic antioxidants like butylated hydroxyanisole (BHA), butylated hydroxyl toluene (BHT), Ascorbic acid and gallic acid appear to be associated with hepatotoxicity and many others negative health effects. Therefore, natural antioxidants may be preferred over the synthetic antioxidants.

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Keywords

Alcoholic extract, Antioxidant activity, Bauhinia variegata Linn, Scavenging potential, Stem bark

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Issue
Vol 11 No 1 (2019)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Evaluation of antioxidant potential of alcoholic stem bark extracts of Bauhinia variegata Linn. (2019). Journal of Applied and Natural Science, 11(1), 235-239. https://doi.org/10.31018/jans.v11i1.2015