Determination of anticholinesterase and antioxidant activities of methanol and water extracts of leaves and fruits of Chamaerops humilis L.

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Published Mar 10, 2019
DOI: https://doi.org/10.31018/jans.v11i1.2001

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Aicha Bouhafsoun
Laboratoire de Production et valorisation végétale et microbienne, Département de Biotechnologie, Faculté SNV, Université des Sciences et Technologie Mohamed Boudiaf (USTOMB), Bp 1505 El M’naouer Oran 31000, Algérie
Mehmet Boga
Department of Pharmaceutical Technology, Faculty of Pharmacy, Dicle University, Diyarbakir, 21280, Turkey
Ahmed Boukeloua
Département des sciences de la nature et de la vie, Faculté des sciences exactes et sciences de la nature et de la vie, Université Larbi ben M’Hidi Oum-EL-Bouaghi, Oum-EL-Bouaghi, Algérie
Hamdi Temel
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Dicle University, Diyarbakir, 21280, Turkey
Meriem Kaid-Harche
Laboratoire de Production et valorisation végétale et microbienne, Département de Biotechnologie, Faculté SNV, Université des Sciences et Technologie Mohamed Boudiaf (USTOMB), Bp 1505 El M’naouer Oran 31000, Algérie

Abstract

The present study was carried out to investigate the antioxidant activity of the water and methanol extracts of leaves and fruits extracts of Chamaerops humilis L. by using ABTS cation radicals and cupric reducing antioxidant capacity (CUPRAC). Anticholinesterase effect of the extracts was tested against both AChE and BChE using a microplate-reader assay based on the Ellman method. The methanol extracts of C. humilis leaves contained relatively higher content of flavonoids and total phenolics than those of fruits. All the extracts were found to have different levels of antioxidant activity in the systems tested. The leave extract showed the highest value of antioxidant activity, based on ABTS radical-scavenging activity, while the fruit water extract showed the highest value (0.53±0.50 µg/mL) of cupric reducing antioxidant activity.  Our data indicates that both methanol and water fruit extract were active for BChE inhibition (31.65 ± 0.37 and 30.19 ± 0.56%) respectively, whereas, all leave extracts did not show any activity against BChE. The present study demonstrated that the methanol and water extracts fractions of C. humilis have different responses with different antioxidant methods. Our results suggest that the C. humilis could be used as a source of antioxidant agents and may be beneficial in the AD treatment.

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Keywords

Biological activity, Chamaerops humilis L., Methanol extract, Secondary metabolites, Water extract

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Issue
Vol 11 No 1 (2019)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Determination of anticholinesterase and antioxidant activities of methanol and water extracts of leaves and fruits of Chamaerops humilis L. (2019). Journal of Applied and Natural Science, 11(1), 144-148. https://doi.org/10.31018/jans.v11i1.2001