Sikkim mandarin (Citrus reticulata) is most important cash crops of Sikkim Himalaya, a tiny state in North East India. The fruit is usually peeled off and eaten as desert used for extraction of juiceor processed for other products. The peel is thrown as waste, though it is rich commercially important essentialoil andpectin. The pectin can be obtained from the pulp waste after extraction of essential oil. The essential oil and pectin content is the effect of the climatic functions. In Himalayas, there is abrupt change in microclimate with change in the altitude. C. reticulata in Sikkim Himalayas grows at the altitudinal range of 800 to 1800 metre from mean sea level. During the present studies pectin was extracted from peel waste after extraction of essential oil. The fruits were collected from five different altitude range viz: 800-1000m, 1000-1200m, 1200-1400m, 1400-1600m and >1600m. FTIR works on the basis of functional group showed range from 3607 cm?1 (O-H stretch region) to 748cm?1 (C-H bend) in mature stage and 3585 cm?1(O-H stretch) to 883 (C-Cl stretch)cm?1 in immature stage. Moreover essential oil showed different compound identification. Limonene was found to be the highest at >1600m altitude (88.46%) at mature stage and (89.06%) at immature stage respectively. These variation may be due to different climatic condition and soil of the elevation. The overall results showed that the pectin can be beneficial for industrial use as well as in pharmaceutical health promotion and treatment. Further peel of the species can be evaluated for its rich content of limonene by different industries.
Altitude, Essential oil, Pectin, Pulp waste, Sikkim mandarin (Citrus reticulata)
Bagde, P.P., Dhenge, S., and Bhivgade, S. (2017).Extraction of pectin from orange peel and lemon peel. International Journal of Engineering Technology Science and Research, 4 (3): 1-7
Brouns, F., Theuwissen, E., Adam, A., Bell, M., Berger, A., and Mensink, R.P.(2012). Cholesterol-lowering properties of different pectin types in mildly hyper-cholesterolemic men and women. European Journal of Clinical Nutrition, 66(5): 591-900.
Chin, L.S., Chin, N.L., andYusof, Y.A. (2014). Extraction and characterization of pectin from passion fruit peels. Agriculture and Agricultural Science Procedia, 2: 231-236.
Devi, W.E., Shukla, R.N., Abraham, A., Jarpula, S., and Kaushik, U. (2014). Optimized Extraction condition and characterization o f pectin from orange Peel, IJREAT, 2: 1–9.
Fakayode, O.A., and Abobi, K. E.(2018).Optimization of oil and pectin extraction from orange (Citrus sinensis) peels: a response surface approach. Journal of Analytical Science and Technology, 9: 20
Ismail, A., Lukman, S., Ojo, S. O., Bolorunduro, K. A., Adeosun, O. O., andOke, I. A. (2016). Solutions of selected pseudo loop equations in water distribution network using microsoft excel solver. Ife Journal of Science, 18 (2): 371-387
Javed, S., Javaid, A., Nawaz, S., Saeed, M.K., Mahmood, Z., Siddiqui, S.Z and Ahmad, R.2014.Phytochemistry, GC-MS Analysis, Antioxidant and Antimicrobial Potential of Essential Oil From Five Citrus Species. Journal of Agricultural Sciences, 6 (3): 201-208.
Joslyn, M.N. (1980). Methods of food analysis, physical chemical and instrumentation method of analysis. Academic Press, New York,5, 67-70.
Joye, D.D. and G.A. Luzio, 2000. Process for Selective Extraction of Pectin from Plant Material by Differential pH. Journal of Carbohydrate Polymer, 43(4): 337-342.
Kamal, G. M., Anwar, F., Hussain, A. I., Sarri, N., and Ashraf, M. Y. (2011).Yield and chemical composition of Citrus essential oils as affected by drying pre treatment of peels. International Food Research Journal,18(4): 1275-1282
Kanmani, P., Dhivya, E., Aravind, J., and Kumaresan, K. (2014). Extraction and Analysis of Pectin from Citrus Peels: Augmenting the yield from Citrus limon using statistical experimental design. Iranica Journal of Energy and Environment, 5 (3):303-312
Khule, R.N., Nitin, B.M., Dipak, S.S., Manisha, M.R., and Sanjay, R.C.(2012). Extraction of pectin from citrus fruit peel and use as natural binder in paracetamol tablet. Scholars Research Library, 4(2):558-564.
Krishnamurthi, C.R., and Giri, K.V. (2003). Preparation, purification and composition of pectin from Indian fruits and vegetables. Brazilian Archives of Biology and Technology, 44: 476-483.
Kulkarni, G.T., Gowthmarajan, K., Rao, B., and Suresh, B.(2006). Evaluation of Binding Properties of Blantago ovate and Trigonella foenum graecum mucilages. Indian Drugs,39(8), 422-425.
Norziah, M.H., Fang, E.O.,andKarim, A.A.(2000). Extraction and characterization of pectin from pomelo fruit peels. In P.A. Williams (Ed.), Gums and stabilisers for the food industry, Cambridge, UK: The Royal Society of Chemistry, 10: 26-36.
Owens, H.S., Mc Cready, R.M., Shepherd, A.D., Schultz, S.H., Pippen, E.L., Swenson, H.A.,.Miers, J.C., Erlandsen, R.F.,andMaclay, W.D. (1952). Methods used at western regional research laboratory for extraction and analysis of pectic materials, AIC-340, Western Regional Research Laboratory, Albany California.
Pagan, J., Ibarz, A., Llorca, M., and Paga, A. (2001). Extraction and characterization of pectin from for the extraction of pectin from stored peach pomace. Food Research International, 34: 605-612
Ranganna, (1986). Handbook of analysis and quality control for fruit and vegetable products. Tata McGraw-Hill Education Private Limited. New Delhi, p 33.
Schultz. (1976).Methods in Carbohydrate Chemistry. In T. Schultz, Methods in Carbohydrate Chemistry, New York, Academic Press, pp 189.
Tobias, N.E., Eke, N.V., Okechukwu, R.I., Nwoguikpe, R.N.,andDuru, C.M. (2011). Waste to health: Industrial raw materials. Potential of peels of Nigerian sweet orange (Citrus sinensis). African Journal of Biotechnology, 10(33): 6257-6264.
Tiwari, A.K., Saha, S.N., Yadav, V.P., Uadhyay, U.K., Katiyar, D., and Mishra, T. (2017).Extraction and characterization of pectin from orange peels. International Journal of Biotechnology and Biochemistry, 13: 39–47.
Wu, P.S., Kuo, Y.T., Chen, S.M., Li, Y ., and Lou, S. B.(2014). Gas Chromatography-Mass Spectrometry Analysis of Photosensitive Characteristics in Citrus and Herb Essential Oils. Journal of Chromatrography Separation Techniques, 6(1): 1-9.
Yadav, S.D, Bankar, N.S., Waghmare, N.N., andShete, D.C. (2017). Extraction and characterization of pectin from sweet lime. 4th International Conference on Multidisciplinary Research and Practice, pp. 58–63.
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