Characterization of pectin extracted from Citrus reticulata L. Blanco collected from different altitudes of Sikkim Himalaya
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Abstract
Sikkim mandarin (Citrus reticulata) is most important cash crops of Sikkim Himalaya, a tiny state in North East India. The fruit is usually peeled off and eaten as desert used for extraction of juiceor processed for other products. The peel is thrown as waste, though it is rich commercially important essentialoil andpectin. The pectin can be obtained from the pulp waste after extraction of essential oil. The essential oil and pectin content is the effect of the climatic functions. In Himalayas, there is abrupt change in microclimate with change in the altitude. C. reticulata in Sikkim Himalayas grows at the altitudinal range of 800 to 1800 metre from mean sea level. During the present studies pectin was extracted from peel waste after extraction of essential oil. The fruits were collected from five different altitude range viz: 800-1000m, 1000-1200m, 1200-1400m, 1400-1600m and >1600m. FTIR works on the basis of functional group showed range from 3607 cm?1 (O-H stretch region) to 748cm?1 (C-H bend) in mature stage and 3585 cm?1(O-H stretch) to 883 (C-Cl stretch)cm?1 in immature stage. Moreover essential oil showed different compound identification. Limonene was found to be the highest at >1600m altitude (88.46%) at mature stage and (89.06%) at immature stage respectively. These variation may be due to different climatic condition and soil of the elevation. The overall results showed that the pectin can be beneficial for industrial use as well as in pharmaceutical health promotion and treatment. Further peel of the species can be evaluated for its rich content of limonene by different industries.
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Altitude, Essential oil, Pectin, Pulp waste, Sikkim mandarin (Citrus reticulata)
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