Study of different isozymes in Pinus gerardiana Wall.

Article Sidebar

PDF
Published Mar 10, 2019
DOI: https://doi.org/10.31018/jans.v11i1.1987

Article Main

Mamata Ranot
Department of Agriculture, SGTB Khalsa College, Sri Anandpur Sahib- 140118 (Punjab), India
Rajesh Sharma
Himalayan Forest Research Institute, conifer campus, Panthaghati, Shimla-171013 (H.P.), India

Abstract

Pinus gerardiana (Chilgoza) is a small to medium sized evergreen tree yielding a highly valuable edible nut. The present investigations were undertaken to assess the different isozymes in Chilgoza pine. Seven isozymes viz., MDH (Malate dehydrogenase), GDH (Glutamate dehydrogenase), SKDH (Shikimic acid dehydrogenase), 6PGDH
(6-Phosphogluconate dehydrogenase), MNR (Menadione reductase), IDH (Isocitrate dehydrogenase) and ADH (Alcohol dehdrogenase) were studied. A total of sixteen gene loci were recorded for seven enzyme systems out of which 6 loci namely MDH- A, 6PGDH- A, SKDH- A, IDH- B, MNR- B and ADH- A were polymorphic whereas the
remaining ten gene loci i.e. MDH- B, MDH- C, MDH- D, GDH- A, 6PGDH- B, SKDH- B, IDH- A, MNR- A, MNR- B and ADH- B showed no variation. The percentage of polymorphic loci and average number of alleles per locus are one of the useful criterions for comparing species of populations for genetic diversity.

Article Details

Article Details

Keywords

Pinus gerardiana, Isozymes, Alleles, Loci

References
Anonymous. (1992). Enzyme Nomenclature. Academic Press, San Diego, California, ISBN 0-12- 227164-5
Bakshi, M. and Konnert, M. (2011). Genetic diversity and differentiation through isozymes in natural populations of Pinus wallichiana A. b. Jacks (Blue Pine) in India. Annals of forest Research 54 (1): 23-27.
Bednorz, L., Myczko, L., Kosinski, P. (2006). Genetic variability and structure of the wild service tree (Sorbus torminalis (L.) Crantz) in Poland. Silvae Genetica 55 (4-5): 197-201.
Fallour, D., Fady, B. and Lefevre, F. (2001). Evidence of variation in segregation patterns within a Cedrus population. The American Genetic Association 92: 260-266.
Gülbaba, A. G. and Özkurt, N. (2002). Isozyme diversity in Cedar (Cedrus libani A. Rich.) populations sampled from the Bolkar mountains. Ministry of Forestry, publication no: 191, Eastern Mediterranean Forestry Research Institute Publ. no: 23, Tech. Bull. no: 14, p. 30 (in Turkish, English summary).
Kurt, Y., Kaya, N. and Isik, K. (2008). Isozyme variation in four natural populations of Cedrus libani A. Rich. In Turkey. Turk. J. Agric. For. 32: 137- 145.
Liensgri. (1990). A manual for starch gel electrophoresis (Ph.D Thesis).
Rawat, K., Kumar, P. and Nautiyal, S. (2006). Variability studies in different seed sources of Pinus wallichiana A.B. Jacks. (Blue pine) in India with special reference to seed and germination characteristics. In: Studies on Himalayan Pines, Negi, S.S., Srivastava, R.K. and Nautiyal, S. (eds.), FRI, Dehradun. pp.64-79
Sharma, R., Khurana, D. K. and Schute, G. (2008). Allozyme variation in populations of Pinus roxburghii sargent in western himalayas. Forest Biotechnology in India. pp.121-136.
White, T. L., Adams, W. T. and Neale, D. B. (2007). Forest genetics. CAB, Publ.,Cambridge MA, USA.
Issue
Vol 11 No 1 (2019)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Study of different isozymes in Pinus gerardiana Wall. (2019). Journal of Applied and Natural Science, 11(1), 116-120. https://doi.org/10.31018/jans.v11i1.1987