Study of different isozymes in Pinus gerardiana Wall.

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Published Mar 10, 2019
DOI: https://doi.org/10.31018/jans.v11i1.1987

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Mamata Ranot
Department of Agriculture, SGTB Khalsa College, Sri Anandpur Sahib- 140118 (Punjab), India
Rajesh Sharma
Himalayan Forest Research Institute, conifer campus, Panthaghati, Shimla-171013 (H.P.), India

Abstract

Pinus gerardiana (Chilgoza) is a small to medium sized evergreen tree yielding a highly valuable edible nut. The present investigations were undertaken to assess the different isozymes in Chilgoza pine. Seven isozymes viz., MDH (Malate dehydrogenase), GDH (Glutamate dehydrogenase), SKDH (Shikimic acid dehydrogenase), 6PGDH
(6-Phosphogluconate dehydrogenase), MNR (Menadione reductase), IDH (Isocitrate dehydrogenase) and ADH (Alcohol dehdrogenase) were studied. A total of sixteen gene loci were recorded for seven enzyme systems out of which 6 loci namely MDH- A, 6PGDH- A, SKDH- A, IDH- B, MNR- B and ADH- A were polymorphic whereas the
remaining ten gene loci i.e. MDH- B, MDH- C, MDH- D, GDH- A, 6PGDH- B, SKDH- B, IDH- A, MNR- A, MNR- B and ADH- B showed no variation. The percentage of polymorphic loci and average number of alleles per locus are one of the useful criterions for comparing species of populations for genetic diversity.

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Keywords

Pinus gerardiana, Isozymes, Alleles, Loci

References
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Issue
Vol 11 No 1 (2019)
Section
Research Articles

This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)

How to Cite

Study of different isozymes in Pinus gerardiana Wall. (2019). Journal of Applied and Natural Science, 11(1), 116-120. https://doi.org/10.31018/jans.v11i1.1987