The aim of the present study was to enumerate the diversity of gut bacterial population of epigeic earthworm species, E. eugeniae exposed to heavy metal. Adult earthworms were treated with three different doses (0.06%, 0.13% and 0.19% w/v) of potassium dichromate (K2Cr2O7) for this purpose. The gut samples were serially diluted and inoculated in seven distinct media viz. Jensen's media, King’smedium B base, Burk’s media, Yeast Mannitol Agar media, Vogel- Johnson agar base media, Luria Bertani Broth Miller and Nutrient agar media. Dose dependent decrease in bacterial population was observed in treated worms when compared with control. Maximum percent change (62.59%) in bacterial population was observed in Burk’s media when exposed to 0.19% chromium. The results revealed that chromium alters the microbial population present in gut of earthworms. Therefore, changes in earthworms’ gut microbial community due to soil management practices can also be used as markers of soil fertility and quality.
Bacterial population, chromium, Earthworm, Media
Cikutovic, M. A., Fitzpatrick, L. C., Venables, B. J. and Goven, A. J.(1993). Sperm count in earthworms (Lumbricusterrestries) as a biomarker for environmental toxicology: effects of cadmium and chlordane. Environmental Pollution,81: 123–125.
Dallinger, R. and Rainbow, P.S. (1993). Ecotoxicology of Metals in Terrestrial Invertebrates. Lewis Publishers: London.
Dutta, A. and Dutta, H.(2016). Some insights on the effect of pesticides on earthworms. International Research Journal of Environment Sciences, 5: 61-66.
Edwards, C. A. and Bohlen, P. J.(1996). Biology and Ecology of earthworms. Chapman and Hall, London.
Esakkiammal, B. and Lakshmibai, L.(2013). Enumeration of bacterial population in the gut region of Eudriluseugeniae. International Journal of Current Microbiology and Applied Sciences,2(5): 267-270.
Flack, F. M. and Hartenstein, R.(1984). Growth of the earthworm Eisenia fetidaon microorganisms and cellulose. Soil Biology and Biochemistry, 16:491-495
Garbisu, C. and Alkorta, I.(2003). Basic concepts on heavy metal soil bioremediation.The European Journal of Mineral Processing and Environmental Protection, 3: 58-66.
Kennette, D., Hendershot, W., Tomlin, A. and Sauve, S.(2002). Uptake of trace metals by the earthworm LumbricusterrestrisL. in urban contaminated soils. Applied Soil Ecology, 19: 191–198.
Martin, J.P. (1950). Use of acid rose Bengal and streptomycin in plate method for estimating soil fungi. Soil Science, 69: 215-232.
Morgan, J. E. and Morgan, A. J.(1999). The accumulation of metals (Cd, Cu, Pb, Zn and Ca) by two ecologically contrasting earthworm species (LumbricusrubellusandAporrectodeacaliginosa): implications for ecotoxicological testing. Applied Soil Ecology,13: 9–20.
Nannipieri, P. and Badalucco, L.(2003). Handbook of processes and modelling in the soil-plant system. Biological processes. In: Benbi DK, Niedere R (eds) Haworth, Binghamton, N.Y. pp 57–82.
Parthasarathi, K. and Ranganathan. L S.(2000). Influence of pressmud on the development of the ovary, oogenesis and the neurosecretory cells of the earthworm, Eudriluseugeniae(Kinberg). African Zoology,35(2): 281-286.
Parthasarathi, K., Ranganathan, L.S., Anandi, V. and Zeyer, J.(2007). Diversity of microflora in the gut and casts of tropical composting earthworms reared on different substrates.Journal of Environmental Biology,28(1): 87-97.
Ranganathan, L. S. and Parthasarathi, K.(1999). Precocious development of LampitomauritiiandEudriluseugeniaereared in pressmud.Pedobiologia, 43: 904-908.
Rangasamy, S., Alagirisamy, B., Purushothaman, G. and Santiago, M.(2013). Effect of vermicompost on biotransformation and bioavailability of hexavalent chromium in soil. IOSR Journal of Agriculture and Veterinary Science,5(3): 34-40.
Ravindran, B., Contreras-Ramos, S. M. and Sekaran, G. (2015). Changes in earthworm gut associated enzymes and microbial diversity on the treatment of fermented tannery waste using epigeic earthworm Eudriluseugenia.Ecological Engineering, 74:394–401
Sampedro, L. and Whalen, J. K.(2007). Changes in the fatty acid profiles through the digestive tract of the earthworm Lumbricusterrestris. Applied Soil Ecology,35: 226–236.
Shefali., Gupta, R. K. and Yadav, J.(2018). Reproductive parameters as assessment tools for arsenic and chromium inducedtoxicity in Eudriluseugeniae. Indian Journal of Agricultural Research,52(6):676-680
Sonia, V., Felix, S. and Antony, C.(2016). Comparative study of growth and reproduction of earthworm Eudriluseugeniae in different organic substrate. International Journal of Applied Sciences, 4: 61-68.
Torsvik, V. and Ovreas, L.(2002). Microbial diversity and function in soil: from genes to ecosystems. Current Opinion in Microbiology,5: 240-245.
Verma, R. and Dwivedi, P.(2013). Heavy metal water pollution- A case study. Recent Research in Science and Technology,5(5): 98-99.
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