Analysis of physico-chemical parameters of soil and population density of Azospirillum isolates from paddy fields of Theni district, Tamilnadu
Article Main
Abstract
The rhizosphere region of eight different paddy field areas of Theni district was studied for their physico-chemical analysis of soil and the population density of Azospirillum sp. The study results showed range of values of pH (7.4-7.9), bulk density (1.13g/cm3 -1.60g/cm3), water holding capacity (34.31% - 18.25%), electrical conductivity (1.31 – 1.11), organic carbon (0.93% - 0.71%) . The macronutrient values namely total nitrogen (1.72% - 0.78%), phosphorus content (0.177% - 0.122%) and potassium (1.364% - 1.273%) were observed. Also micronutrients of various paddy fields like Zn (0.9% - 0.5%), Cu (2.3% - 1.7%), Fe (10.9% -8.2%), Mn (6.7% - 5.2%) were recorded, whereas the values of available macronutrients namely nitrogen (295kg/ha - 223kg/ha), phosphorous (89Kg/acre - 49kg/acre), potassium (790kg/acre - 490kg/acre) were noted. The Azospirillum population density was highest in Chinnamanur (192 x105 CFU/g) and lowest in Royyanpatti (91 x105 CFU/g). Thus through this work we were able to isolate and identify novel high yielding Azosprillium sp from paddy fields of Theni district.
Article Details
Article Details
Keywords
Azospirillum, Paddy, Population density, Rhizosphere, Soil, Theni
References
Ketterings, Q.M., Blair, J.M. and Marinissen, J.C.Y. (1997). Effects of earthworms on soil aggregate stability and carbon and nitrogen storage in a legume cover crop agroecosystem. Soil Biol. Biochem. 29, 401-408.
Paoletti, M. G. (1999). The role of earthworms for assessment of sustainability and as bioindicators. – Agriculture. Ecosystems, Environment. 74, p. 37-155.
Narayan, R and Gupta, N.C., 2018. Isolation, cultural and physiological characterisation of Azospirillum from acidic soils of Ranchi. J Pharmacognosy and Phytochem. 7(4): 2611-2617.
Ivask, M., Kuu, A., Sizov, E. (2008). Abundance of earthworm species in Estonian arable soils. Eur. J. Soil Biol. 43: S39-S42.
Haiyambo. D.H., and Chimwamurombe, P.M., 2018. Isolation of the Azospirillum Species from the Rhizosphere of the Leguminous Bauhinia petersiana in North Eastern Namibia. Jordan J Bio Sci. 11, (4): 347 – 353.
Lopez-de-Victoria, G., 1989. Chemotactic behaviour of deep subsurface bacteria toward carbohydrates, amino acids and a chlorinated alkene. M.Sc., Thesis University of Pureto Rica. Rio Piedras.
Mohamed, M.G., Mohamed H.M., Gameh M., El-Rewainy H.M., 2018. Isolation and Characterization of Azospirillum Isolates from Soil and Their Effect on Growth and Yield of Wheat (Triticum aestivum L.) under Different Levels of Nitrogen Fertilizer. Assiut J. Agric. Sci. 49(3):107-116.
Jackson, M. L., 1973, Soil Chemical Analysis, Prentice Hall of India Private Limited, New Delhi.
Walkley, A.J. and Black, I.A. (1934) Estimation of soil organic carbon by the chromic acid titration method. Soil Sci. 37, 29-38.
Subbiah, B.V. and Asija, G.L. (1956). A rapid procedure for estimation of available nitrogen in soils. Curr. Sci., 25: 259-260.
Stanford, S. and English, L, 1949. Use of flame photometer in rapid soil tests for K and Ca. Agron. ]., 41: 446-447.
Muthuvel, P. and Udayasoorian, C., 1999. Soil, plant, water are agrochemical analysis. Tamilnadu. Agricultural Unviersity, Coimbatore, India.Lindsay and Norvell, 1978.
Lindsay, W.L. and Norvell, W.A. (1978) Development of a Dtpa Soil Test for Zinc, Iron, Manganese, and Copper. Soil Science Society of America Journal, 42, 421-428.
Baldani, V. L. D. and Dobereiner, J., 1980, Host-plant specificity in the infection ofcereals with Azospirillum sp. Soil Biol. Bioche.,12:86-90.
Rodriguez Cacéres E. A. (1982) Improved medium for isolation of Azospirillum spp. Applied and Environmental Microbiology 44, 990-991.
Vaneet Kumar, Sandip Singh Bhatti and Avinash Kaur Nagpal, 2016. Seasonal analysis of physico-chemical parameters of agricultural soil samples collected from banks of rivers Beas and Sutlej, Punjab, India. Journal of Chemical and Pharmaceutical Research, 8(8):439-449.
Chahal V., C Piar; A Nagpal; JK Katnoria; YB Pakade, Evaluation of heavy metals contamination and its genotoxicity in agricultural soil of Amritsar, Punjab, India. International Journal of Research in Chemistry and Environment, 2014, 4, 20-28.
Rashidi M; M Seilsepour. Modeling of soil total nitrogen based on soil organic carbon. Journal of Agricultural and Biological Science, 2009, 4, 1-5.
Calalang GD; L Bock; G Colinet. Establishing a soil reference system for fertility assessment and monitoring at plot level in the high lands of Mindanao, Philippines. Eurasian Journal of Soil Science, 2014, 3, 189-196.
Bharadwaj A; V Khandelwal; P Choudhary; AK Bhatia. Comparative studies of organic enrichers in the improvement of physicochemical and microbiological characteristics of saline/usar soils. Journal of Chemical and Pharmaceutical Research, 2011, 3, 997-1003.
Perveen S, Tariq M, Farmanullah J, Khattak K, Hamid A (1993). Study of micronutrient status of some important sites of NWFP Pakistan., Sarhad J Agri, 9:467-473.
Sumathi CS, Balasubramanian V, Ramesh N, Rajesh Kannan V (2008). Influence of biotic and abiotic features on Curcuma longa L. plantation under tropical condition., Middle-East J Sci Res, 3:171-178.
Akbar MH, Ahmed OH, Jamaluddin AS, Nik Ab. Majid NM, Abdul-Hamid H, Jusop S, Hassan A, Yusof KH, Abdu A (2010). Differences in soil physical and chemical properties of rehabilitated and secondary forests., American J Appl Sci, 7:1200-1209.
Senthil Kumar. R and Panneerselvam. A (2013). A Studies on Azospirillum isolated from the soils of Thiruvarur Dt., Tamilnadu, India. Adv. Appl. Sci. Res., 4(1):86-93.
PrameenaSheeja (2015). Assessment of macro and micronutrients in soils from Mannargudi Area, Thiruvarur, India. Res. J. Chem. Environ. Sci. 3, 6;32-37.
Kanimozhi, K and Panneerselvam, A., 2011. Investigation of soil characters and Azospirillum isolated from paddy soils of Thanjavurdistrict, East Coast of Tamilnadu, India. Archieves of Applied Science Research, 3 (2):525-536.
Cristyakova, I.K and Kalininskaya, TA., 1984. Azospirillum typically occur in the rhizosphere of vascular plants. Ser. Biol., 1: 149 – 153.
Attitalla, I. H., Abobaker, M. A., Muftah, A. N., Amir, H. G., Latiffah, Z., Hasnah, M. J., Ibrahim, A. A. and Baharuddin, S., 2010, Occurrence and microbiological characteristics of Azospirillumstrains associated with leguminous and nonleguminous plants in Al jabal Al Akhdar eco- Region, Libya. American- Eurasian J. Agric. Environ. Sci., 8(6): 617-625.
Ki-Yoon Kim, H. P. Deka Boruah, Chung-Woo Kim, C. C. Shagol and Tong-Min Sa, 2010, Isolation and evaluation of inoculation effect of Azospirillum sp. on growth, colonization and nutrient uptake of crops under green house condition. 19th World Congress of Soil Science, 1-6 August 2010, Brisbane, Australia.
Paoletti, M. G. (1999). The role of earthworms for assessment of sustainability and as bioindicators. – Agriculture. Ecosystems, Environment. 74, p. 37-155.
Narayan, R and Gupta, N.C., 2018. Isolation, cultural and physiological characterisation of Azospirillum from acidic soils of Ranchi. J Pharmacognosy and Phytochem. 7(4): 2611-2617.
Ivask, M., Kuu, A., Sizov, E. (2008). Abundance of earthworm species in Estonian arable soils. Eur. J. Soil Biol. 43: S39-S42.
Haiyambo. D.H., and Chimwamurombe, P.M., 2018. Isolation of the Azospirillum Species from the Rhizosphere of the Leguminous Bauhinia petersiana in North Eastern Namibia. Jordan J Bio Sci. 11, (4): 347 – 353.
Lopez-de-Victoria, G., 1989. Chemotactic behaviour of deep subsurface bacteria toward carbohydrates, amino acids and a chlorinated alkene. M.Sc., Thesis University of Pureto Rica. Rio Piedras.
Mohamed, M.G., Mohamed H.M., Gameh M., El-Rewainy H.M., 2018. Isolation and Characterization of Azospirillum Isolates from Soil and Their Effect on Growth and Yield of Wheat (Triticum aestivum L.) under Different Levels of Nitrogen Fertilizer. Assiut J. Agric. Sci. 49(3):107-116.
Jackson, M. L., 1973, Soil Chemical Analysis, Prentice Hall of India Private Limited, New Delhi.
Walkley, A.J. and Black, I.A. (1934) Estimation of soil organic carbon by the chromic acid titration method. Soil Sci. 37, 29-38.
Subbiah, B.V. and Asija, G.L. (1956). A rapid procedure for estimation of available nitrogen in soils. Curr. Sci., 25: 259-260.
Stanford, S. and English, L, 1949. Use of flame photometer in rapid soil tests for K and Ca. Agron. ]., 41: 446-447.
Muthuvel, P. and Udayasoorian, C., 1999. Soil, plant, water are agrochemical analysis. Tamilnadu. Agricultural Unviersity, Coimbatore, India.Lindsay and Norvell, 1978.
Lindsay, W.L. and Norvell, W.A. (1978) Development of a Dtpa Soil Test for Zinc, Iron, Manganese, and Copper. Soil Science Society of America Journal, 42, 421-428.
Baldani, V. L. D. and Dobereiner, J., 1980, Host-plant specificity in the infection ofcereals with Azospirillum sp. Soil Biol. Bioche.,12:86-90.
Rodriguez Cacéres E. A. (1982) Improved medium for isolation of Azospirillum spp. Applied and Environmental Microbiology 44, 990-991.
Vaneet Kumar, Sandip Singh Bhatti and Avinash Kaur Nagpal, 2016. Seasonal analysis of physico-chemical parameters of agricultural soil samples collected from banks of rivers Beas and Sutlej, Punjab, India. Journal of Chemical and Pharmaceutical Research, 8(8):439-449.
Chahal V., C Piar; A Nagpal; JK Katnoria; YB Pakade, Evaluation of heavy metals contamination and its genotoxicity in agricultural soil of Amritsar, Punjab, India. International Journal of Research in Chemistry and Environment, 2014, 4, 20-28.
Rashidi M; M Seilsepour. Modeling of soil total nitrogen based on soil organic carbon. Journal of Agricultural and Biological Science, 2009, 4, 1-5.
Calalang GD; L Bock; G Colinet. Establishing a soil reference system for fertility assessment and monitoring at plot level in the high lands of Mindanao, Philippines. Eurasian Journal of Soil Science, 2014, 3, 189-196.
Bharadwaj A; V Khandelwal; P Choudhary; AK Bhatia. Comparative studies of organic enrichers in the improvement of physicochemical and microbiological characteristics of saline/usar soils. Journal of Chemical and Pharmaceutical Research, 2011, 3, 997-1003.
Perveen S, Tariq M, Farmanullah J, Khattak K, Hamid A (1993). Study of micronutrient status of some important sites of NWFP Pakistan., Sarhad J Agri, 9:467-473.
Sumathi CS, Balasubramanian V, Ramesh N, Rajesh Kannan V (2008). Influence of biotic and abiotic features on Curcuma longa L. plantation under tropical condition., Middle-East J Sci Res, 3:171-178.
Akbar MH, Ahmed OH, Jamaluddin AS, Nik Ab. Majid NM, Abdul-Hamid H, Jusop S, Hassan A, Yusof KH, Abdu A (2010). Differences in soil physical and chemical properties of rehabilitated and secondary forests., American J Appl Sci, 7:1200-1209.
Senthil Kumar. R and Panneerselvam. A (2013). A Studies on Azospirillum isolated from the soils of Thiruvarur Dt., Tamilnadu, India. Adv. Appl. Sci. Res., 4(1):86-93.
PrameenaSheeja (2015). Assessment of macro and micronutrients in soils from Mannargudi Area, Thiruvarur, India. Res. J. Chem. Environ. Sci. 3, 6;32-37.
Kanimozhi, K and Panneerselvam, A., 2011. Investigation of soil characters and Azospirillum isolated from paddy soils of Thanjavurdistrict, East Coast of Tamilnadu, India. Archieves of Applied Science Research, 3 (2):525-536.
Cristyakova, I.K and Kalininskaya, TA., 1984. Azospirillum typically occur in the rhizosphere of vascular plants. Ser. Biol., 1: 149 – 153.
Attitalla, I. H., Abobaker, M. A., Muftah, A. N., Amir, H. G., Latiffah, Z., Hasnah, M. J., Ibrahim, A. A. and Baharuddin, S., 2010, Occurrence and microbiological characteristics of Azospirillumstrains associated with leguminous and nonleguminous plants in Al jabal Al Akhdar eco- Region, Libya. American- Eurasian J. Agric. Environ. Sci., 8(6): 617-625.
Ki-Yoon Kim, H. P. Deka Boruah, Chung-Woo Kim, C. C. Shagol and Tong-Min Sa, 2010, Isolation and evaluation of inoculation effect of Azospirillum sp. on growth, colonization and nutrient uptake of crops under green house condition. 19th World Congress of Soil Science, 1-6 August 2010, Brisbane, Australia.
Issue
Section
Research Articles
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) © Author (s)
How to Cite
Analysis of physico-chemical parameters of soil and population density of Azospirillum isolates from paddy fields of Theni district, Tamilnadu. (2019). Journal of Applied and Natural Science, 11(1), 88-93. https://doi.org/10.31018/jans.v11i1.1972
