Hepatoprotective efficacy of edible macrofungi Dacryopinax spathularia (Schwein) and Schizophyllum commune (Fries) against Carbon tetrachloride induced hepatotoxicity in albino Wistar rats
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Abstract
In the present study the hepatoprotective efficacy of two edible macrofungi Dacryopinax spathularia and Schizophyllum commune has been assessed against CCl4-induced hepatotoxicity in albino wistar rats. The administration of CCl4(1ml/Kg) resulted into significant (p<0.05) rise in the levels of liver function marker enzymes Alanine Transaminase (ALT), Aspartate Aminotransferase (AST), Alkaline Phosphatase (ALP) and bilirubin and decrease in the levels of total protein and albumin in blood. On administration of low dose (250mg/Kg) and high dose (500mg/Kg) of both macrofungal extracts in different hepatotoxic group of rats, the serum levels of AST, ALT, ALP and bilirubin significantly (p<0.05) lowered down and the levels of total protein and albumin significantly (p<0.05) increased in comparison to the hepatotoxic group of rats, reflecting the hepatoprotective impact of both the extracts.
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Keywords
Carbon tetrachloride, Hepatotoxicity, Hepatoprotective, Macrofungi
References
Adeyemi, D.O., Ukwenya, V. O., Obuotor, E.M., Adewole, S.O. (2014). Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model streptozotocin diabetes-induced liver damage. BMC complement. Alt. Med. 14: 277-287.
Andreia, A.S., Anacharis B. de Sa N., Adelar, B., Sandra, M.G. da Costa, Eloa, A.K., Cristina, G.M. de Souza, Rosane, M.P. (2013). Review on Hepatoprotective effects of Mushrooms. Molecules, 18, 7609-7630; doi : 10.3390/molecules18077609.
Barros, L.; Baptista, P.; Estevinho, L.M.; Ferreira, I.C.F.R. (2007). Effect of fruiting body maturity stage on chemical composition and antimicrobial activity of Lactarius sp. Mushrooms. J. Agric. Food Chem. 55, 8766–8771.
Bessey, O.A., Lowry, D.H., Brock, M.J. (1964). A method for the rapid determination of alkaline phosphatase with five cubic millimeter of serum. J. Biol. Chem. 164: 321-326.
Chang, S.T. and Miles, P.G. (2004). Mushrooms cultivation, Nutritional value, Medicinal effect, and Environmental impact. United states: CRC press.
Chatterjee, S., Dey, A., Dutta, R., Dey, S., Acharya, K. (2011). Hepatoprotective effect of the ethanolic extract of Calocybe indica on mice with CCl4 hepatic intoxication . Int. J. Pharm. Tech. Res. 3: 2162-2168.
Maity, T. and Ahmad, A. (2012) Protective effect of Mikania scandens (L.) Willd. against Isoniazid induced hepatotoxicity in rats. Int. J. Pharm. Sci. 4:466-9.
Dandapat, S. and Sinha, M.P. (2015). Antioxidant and anti-inflammatory activity of Pleurotus tuber-regium (Rumph. Ex. Fr.) Singer. Adv. In Biol. Res., 9 (3): 140-145.
Dass, E. and Sattigeri, B.M. (2018). Hepatoprotective effect of DL-Methionine on diclofenac induced hepatotoxicity in albino rats. Int. J. Res. Med. Sci., 6(3), 802-807.
De Groot, H., Littauer, A., Hugo-Wissemann, D., Wissemann, P., Noll, T. (1988). Lipid peroxidation and cell viability in isolated hepatocytes in a redesigned oxystat system: Evaluation of the hypothesis that lipid peroxidation, preferentially induced at low oxygen partial pressure, is decisive for CC14 liver cell injury . Arch Biochem Biophys. 264:591-99.
Edwards, M.J., Keller, B.J., Kauffman, F.C., Thurman, R.G. (1993). The involvement of kupffer cells in carbon tetrachloride toxicity. Toxicol. Appl. Pharmacol. 119: 275-279.
Hu, S.-H.; Wang, J.-C.; Lien, J.-L.; Liaw, E.-T.; Lee, M.-Y. (2006). Antihyperglycemic effect of polysaccharide from fermented broth of Pleurotus citrinopileatus. Appl. Microbiol. Biotechnol. 70, 107–113.
Huo, H.Z., Wang, B., Liang, Y.K., Bao, Y.Y., Gu, Y. (2011). hepatoprotective and antioxidant effects of licorice extract against CCl4-induced oxidative damage in rats. Int. J. Mol. Sci. 12: 6529-6543.
Hussain, F., Malik, A., Ayyaz, U., Shafique, H., Rana, Z., Hussain, Z. (2017). Efficient hepatoprotective activity of Cranberry extract against CCl4-induced hepatotoxicity in wistar albino rat model: Down regulation of liver enzymes and strong antioxidant activity. Asian Pacific J. of Trop. Med., 10(11): 1054-1058.
Jayakumar, T.; Ramesh, E.; Geraldine, P. (2006). Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCL4-induced liver injury in rats. Food Chem. Toxicol. 44, 1989–1996.
Kingsley, S.R. and Frankel, S.J. (1939). The determination of serum total protein albumin and globulin by biuret reaction. J. Biol. Chem. 128:131-137.
Karaman, M., Vesic, M., Stahl, M., Novakovic, M., Janjic, L., Matavuly, M. (2012) Bioactive properties of wild growing mushroom species Ganorderma applanatum (Pers.) pat. From Fruska Gora forest (Serbia). Ethnomed. Ther. Valid. 32: 361-377.
Kodavanti, P.R., Joshi, U.M., Young, Y.A., Meydrech, E.F., Mehendale, H.M. (1989). Protection of hepatotoxic and lethal effects of CCl4 by partial
hepatectomy. Toxicol. Pathol. 17, 494-505.
Kumar, A., Ali, S., Lal, S.B., Sinha, M.P. (2018).
Mycochemical screening and determination of nutritive potency and antioxidant activity of edible macrofungi Dacryopinax spathularia and Schizophyllim commune. World J. of Pharm. Res., 7(16): 1311-1321.
Lindequist, U., Niedermeyer, T.H., Julich, W.D. (2005). The pharmacological potential of mushrooms. Evid Based Complement Alternat. Med., 2:285-289.
Moradali, M.-F.; Mostafavi, H.; Ghods, S.; Hedjaroude, G.-A. (2007). Immunomodulating and anticancer in the realm of macromycetes fungi (macrofungi). Intern. Immunopharmacol., 7:701–724.
Moro, C.; Palacios, I.; Lozano, M.; DÁrrigo, M.; Guillamón, E.; Villares, A.; Martínez, J.A.; García-Lafuente, A. (2012). Anti-inflammatory activity of methanolic extracts from edible mushrooms in LPS activated RAW 264.7 macrophages. Food Chem. 130, 350–355.
OECD (2004). OECD guidelines for the testing of chemicals /section 4: Health effects test no. 423; Acute Oral Toxicity-AcuteTtoxic Class Method.Organisation for Economic Cooperation and
Development.
Peralta, R.M.; Oliveira, A.L.; Eler, G.J.; Soares, A.A.; Bracht, A. (2008). Funcional properties of edible and medicinal mushrooms. Curr. Trends Microbiol. 4:45–60.
Reitman, S. and Frankel, S.A. (1957). Colorimetric method for the determination of serum glutamic oxaloacetic and pyruvic transaminase. Am. J. Clin. Path. 28:56.
Seifert, W.F., Bosma, A., Brouwer, A. (1994). Vitamin A deficiency potentiates CCl4- induced liver fibrosis in rats. Hepatology, 19(1): 193-201.
Stephens, W.E. (2003). Oxidative stress, toxic hepatitis, and antioxidants with particular emphasis on zinc. Exp. Biol. 31: 316-321.
Thawbrew, M.I., Joice, P.D.T.M., Rajatissa, W.A. (1987). Comparative study of efficacy of Patella indica and Osbeckia octandra in the treatment of liver dysfunction. Plant Med. 53: 239-241.
Uru, O.M.Q., Emeka, I.E., Lotanna, A.D., Ogechukwu, U.B. (2013). Hepatoprotective and anti-hepatotoxic activities of aqueous leaf extract of Tacazzea barteria against carbon tetrachloride induced hepatotoxicity in albino rat. Int. Res. J. Pharm. 4: 60-65.
Wasser, S. P., Weis, A. (1999). Medicinal properties of substances occurring in higher basidiomycetes mushrooms : Current perspectives (review). Int. J. Med. Mushrooms 1:31-62.
Andreia, A.S., Anacharis B. de Sa N., Adelar, B., Sandra, M.G. da Costa, Eloa, A.K., Cristina, G.M. de Souza, Rosane, M.P. (2013). Review on Hepatoprotective effects of Mushrooms. Molecules, 18, 7609-7630; doi : 10.3390/molecules18077609.
Barros, L.; Baptista, P.; Estevinho, L.M.; Ferreira, I.C.F.R. (2007). Effect of fruiting body maturity stage on chemical composition and antimicrobial activity of Lactarius sp. Mushrooms. J. Agric. Food Chem. 55, 8766–8771.
Bessey, O.A., Lowry, D.H., Brock, M.J. (1964). A method for the rapid determination of alkaline phosphatase with five cubic millimeter of serum. J. Biol. Chem. 164: 321-326.
Chang, S.T. and Miles, P.G. (2004). Mushrooms cultivation, Nutritional value, Medicinal effect, and Environmental impact. United states: CRC press.
Chatterjee, S., Dey, A., Dutta, R., Dey, S., Acharya, K. (2011). Hepatoprotective effect of the ethanolic extract of Calocybe indica on mice with CCl4 hepatic intoxication . Int. J. Pharm. Tech. Res. 3: 2162-2168.
Maity, T. and Ahmad, A. (2012) Protective effect of Mikania scandens (L.) Willd. against Isoniazid induced hepatotoxicity in rats. Int. J. Pharm. Sci. 4:466-9.
Dandapat, S. and Sinha, M.P. (2015). Antioxidant and anti-inflammatory activity of Pleurotus tuber-regium (Rumph. Ex. Fr.) Singer. Adv. In Biol. Res., 9 (3): 140-145.
Dass, E. and Sattigeri, B.M. (2018). Hepatoprotective effect of DL-Methionine on diclofenac induced hepatotoxicity in albino rats. Int. J. Res. Med. Sci., 6(3), 802-807.
De Groot, H., Littauer, A., Hugo-Wissemann, D., Wissemann, P., Noll, T. (1988). Lipid peroxidation and cell viability in isolated hepatocytes in a redesigned oxystat system: Evaluation of the hypothesis that lipid peroxidation, preferentially induced at low oxygen partial pressure, is decisive for CC14 liver cell injury . Arch Biochem Biophys. 264:591-99.
Edwards, M.J., Keller, B.J., Kauffman, F.C., Thurman, R.G. (1993). The involvement of kupffer cells in carbon tetrachloride toxicity. Toxicol. Appl. Pharmacol. 119: 275-279.
Hu, S.-H.; Wang, J.-C.; Lien, J.-L.; Liaw, E.-T.; Lee, M.-Y. (2006). Antihyperglycemic effect of polysaccharide from fermented broth of Pleurotus citrinopileatus. Appl. Microbiol. Biotechnol. 70, 107–113.
Huo, H.Z., Wang, B., Liang, Y.K., Bao, Y.Y., Gu, Y. (2011). hepatoprotective and antioxidant effects of licorice extract against CCl4-induced oxidative damage in rats. Int. J. Mol. Sci. 12: 6529-6543.
Hussain, F., Malik, A., Ayyaz, U., Shafique, H., Rana, Z., Hussain, Z. (2017). Efficient hepatoprotective activity of Cranberry extract against CCl4-induced hepatotoxicity in wistar albino rat model: Down regulation of liver enzymes and strong antioxidant activity. Asian Pacific J. of Trop. Med., 10(11): 1054-1058.
Jayakumar, T.; Ramesh, E.; Geraldine, P. (2006). Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCL4-induced liver injury in rats. Food Chem. Toxicol. 44, 1989–1996.
Kingsley, S.R. and Frankel, S.J. (1939). The determination of serum total protein albumin and globulin by biuret reaction. J. Biol. Chem. 128:131-137.
Karaman, M., Vesic, M., Stahl, M., Novakovic, M., Janjic, L., Matavuly, M. (2012) Bioactive properties of wild growing mushroom species Ganorderma applanatum (Pers.) pat. From Fruska Gora forest (Serbia). Ethnomed. Ther. Valid. 32: 361-377.
Kodavanti, P.R., Joshi, U.M., Young, Y.A., Meydrech, E.F., Mehendale, H.M. (1989). Protection of hepatotoxic and lethal effects of CCl4 by partial
hepatectomy. Toxicol. Pathol. 17, 494-505.
Kumar, A., Ali, S., Lal, S.B., Sinha, M.P. (2018).
Mycochemical screening and determination of nutritive potency and antioxidant activity of edible macrofungi Dacryopinax spathularia and Schizophyllim commune. World J. of Pharm. Res., 7(16): 1311-1321.
Lindequist, U., Niedermeyer, T.H., Julich, W.D. (2005). The pharmacological potential of mushrooms. Evid Based Complement Alternat. Med., 2:285-289.
Moradali, M.-F.; Mostafavi, H.; Ghods, S.; Hedjaroude, G.-A. (2007). Immunomodulating and anticancer in the realm of macromycetes fungi (macrofungi). Intern. Immunopharmacol., 7:701–724.
Moro, C.; Palacios, I.; Lozano, M.; DÁrrigo, M.; Guillamón, E.; Villares, A.; Martínez, J.A.; García-Lafuente, A. (2012). Anti-inflammatory activity of methanolic extracts from edible mushrooms in LPS activated RAW 264.7 macrophages. Food Chem. 130, 350–355.
OECD (2004). OECD guidelines for the testing of chemicals /section 4: Health effects test no. 423; Acute Oral Toxicity-AcuteTtoxic Class Method.Organisation for Economic Cooperation and
Development.
Peralta, R.M.; Oliveira, A.L.; Eler, G.J.; Soares, A.A.; Bracht, A. (2008). Funcional properties of edible and medicinal mushrooms. Curr. Trends Microbiol. 4:45–60.
Reitman, S. and Frankel, S.A. (1957). Colorimetric method for the determination of serum glutamic oxaloacetic and pyruvic transaminase. Am. J. Clin. Path. 28:56.
Seifert, W.F., Bosma, A., Brouwer, A. (1994). Vitamin A deficiency potentiates CCl4- induced liver fibrosis in rats. Hepatology, 19(1): 193-201.
Stephens, W.E. (2003). Oxidative stress, toxic hepatitis, and antioxidants with particular emphasis on zinc. Exp. Biol. 31: 316-321.
Thawbrew, M.I., Joice, P.D.T.M., Rajatissa, W.A. (1987). Comparative study of efficacy of Patella indica and Osbeckia octandra in the treatment of liver dysfunction. Plant Med. 53: 239-241.
Uru, O.M.Q., Emeka, I.E., Lotanna, A.D., Ogechukwu, U.B. (2013). Hepatoprotective and anti-hepatotoxic activities of aqueous leaf extract of Tacazzea barteria against carbon tetrachloride induced hepatotoxicity in albino rat. Int. Res. J. Pharm. 4: 60-65.
Wasser, S. P., Weis, A. (1999). Medicinal properties of substances occurring in higher basidiomycetes mushrooms : Current perspectives (review). Int. J. Med. Mushrooms 1:31-62.
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Hepatoprotective efficacy of edible macrofungi Dacryopinax spathularia (Schwein) and Schizophyllum commune (Fries) against Carbon tetrachloride induced hepatotoxicity in albino Wistar rats. (2019). Journal of Applied and Natural Science, 11(1), 62-65. https://doi.org/10.31018/jans.v11i1.1959
