The Molecular identification of root nodule bacteria from edible crops of Fabaceae family, Kerala
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Abstract
The root nodule bacteria were isolated from three different leguminous plants belonging to the Fabaceae family such as Cajanus cajan, Lablab purpureus and Vigna unguiculata using YEMA medium and identified. The isolates were submitted to 16S rRNA and PCR–RFLP typing. A representative sample was further submitted to sequence analysis of 16S rRNA. Isolates were assigned to two genera and were related to Rhizobium spp. (75%) and Bacillus spp. (25%) respectively. This study opens the doors to researchers to do specific studies on this root nodule bacteria which are symbiotic with these legume plants in this area for their antibacterial activity, medicine and food application, and sustainable agriculture. Rhizobium and other beneficial microbes may be used as biofertilizers as a substitute for chemical fertilizers.
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Keywords
Fabaceae, Isolation, Leguminous, PCR-RFLP, Root nodule bacteria, 16S rRNA
References
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Ramezanpour, M.R. (2011). Biochemical characteristics and genetic diversity of fluorescent pseudomonads isolated from rice rhizosphere in north of Iran. Am. Eurasian J. Agric. Environ. Sci., 10:180–185.
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Sa?di S, Mnasri B, Mhamdi R (2011). Diversity of nodule endophytic agrobacteria-like strains associated to different grain legumes in Tunisia. Syst. Appl. Microbiol., 34: 524–530.
Vessey, J.K. and Chemining'wa, G.N. (2006). The genetic diversity of Rhizobium leguminosarum bv. viciae in cultivated soils of the eastern Canadian prairie. Soil Biol. Biochem., 38 : 153-163.
Wadhwa1 Z., Srivastava V., Rani R., Tanvi, Makkar K. and JangraS.(2017) Isolation and Characterization of Rhizobium from Chickpea (Cicer arietinum) Int. J. Curr. Microbiol. App. Sci., 6 (11): 2880-2893
Younes, R.E, et al., (2005). Identification of Rhizobium-specific intergenic mosaic elements within an essential two-component regulatory system of Rhizobium species. Journal of Bacteriology, 177 : 5485- 5494.
Dariel, B. and John, G. (2003). Rhizobium, A Manual for Basic Practical Microbiology, Society for General Microbiology, London.
Lajudie, P., Willems, A., Nick, G., Mohamed, T.S., Torck, U., Filali- Maltouf, A., Kersters, K., Dreyfus, B., Lindstrom, K. and Gillis, M. (1999). Agrobacterium bv.1 strains isolated from nodules of tropical legumes. Syst. Appl. Microbiol., 22:119–132.
Erman, M., Demir, S., Ocak, E., Tufenkci, S., Oguz, F. & Akkopru, A. (2011). Effects of Rhizobium, arbuscular mycorrhiza and whey applications on some properties in chickpea (Cicer arietinum L.) under irrigated and rainfed conditions: Yield, yield components, nodulation and AMF colonization. Field Crops Res., 122(1), 14-24.
FAO.(2012). Grassland species index. Cajanus cajan.
Ibanez F, Angelini J, Taurian T, Tonelli ML, Fabra A (2009). Endophytic occupation of peanut root nodules by opportunistic Gamma proteobacteria. Syst. Appl. Microbiol., 32:49–55
Kurlovick, B.S, et al., (1997). Stress tolerance in Rhizobium and Bradyrhizobium, and nodulation under adverse soil conditions. Canadian Journal of Microbiology, 38, 475-484.
Maass, B.L., M.R. Knox, S.C. Venkatesha, T.T. Angessa, S. Ramme, and B.C. Pengelly. (2010). L. purpureus-a crop lost for Africa? Trop. Plant Biol., 3(3):123–135. doi:10.1007/s12042-010-9046-1
Mhamdi R, Laguerre G, Aouani ME, Mars M, Amarger N (2002). Different species and symbiotic genotypes of field rhizobia can nodulate Phaseolus vulgaris in Tunisian soils. FEMS Microbiol. Ecol., 41:77–84.
Muresu R, Maddau G, Delogu G, Cappuccinelli P, Squartini A (2010). Bacteria colonizing root nodules of wild legumes exhibit virulence-associated properties of mammalian pathogens. Antonie Van Leeuwenhoek, 97:143–153.
Phatak, S.C., R.G. Nadimpalli, S.C. Tiwari, and H.L. Bhardwaj. (1993). Pigeonpeas: potential new crop for the southeastern United States. In: J. Janick and J.E. Simon, editors, New Crops. Wiley, New York. p.597–599.
Rajeswari P., Aishwaryaalakshmi B. Jeyagowri C. (2017). Isolation, identification and screening of Rhizobium for plant growth promotion. Int. J. of App. Res., 2017; 3(1): 732-733.
Ramezanpour, M.R. (2011). Biochemical characteristics and genetic diversity of fluorescent pseudomonads isolated from rice rhizosphere in north of Iran. Am. Eurasian J. Agric. Environ. Sci., 10:180–185.
Robertson, G and Groffman P.(2015). Nitrogen transformations In: Soil microbiology, ecology and biochemistry, E. A. Paul, editor. Fourth edition. Academic Press, Burlington, Massachusetts, USA. Pages 421-446.
Sa?di S, Mnasri B, Mhamdi R (2011). Diversity of nodule endophytic agrobacteria-like strains associated to different grain legumes in Tunisia. Syst. Appl. Microbiol., 34: 524–530.
Vessey, J.K. and Chemining'wa, G.N. (2006). The genetic diversity of Rhizobium leguminosarum bv. viciae in cultivated soils of the eastern Canadian prairie. Soil Biol. Biochem., 38 : 153-163.
Wadhwa1 Z., Srivastava V., Rani R., Tanvi, Makkar K. and JangraS.(2017) Isolation and Characterization of Rhizobium from Chickpea (Cicer arietinum) Int. J. Curr. Microbiol. App. Sci., 6 (11): 2880-2893
Younes, R.E, et al., (2005). Identification of Rhizobium-specific intergenic mosaic elements within an essential two-component regulatory system of Rhizobium species. Journal of Bacteriology, 177 : 5485- 5494.
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How to Cite
The Molecular identification of root nodule bacteria from edible crops of Fabaceae family, Kerala. (2018). Journal of Applied and Natural Science, 10(3), 1063-1065. https://doi.org/10.31018/jans.v10i3.1864
