Effect of Aloe vera juice on the hepatotoxicity induced by isoniazid drug
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Abstract
Aloe vera leaves were gently pressed and the juice obtained was collected in a sterile container. The yield was calculated based on weight of the extract compared to the weight of the leaves. Twenty (20) Wistar strain rats (average weight 120-150 g) were divided into four (4) groups containing five (5) rats in each group. Each group was fed on a different diet like the control rats were fed on commercial rat pellet; experimental rats fed orally with Aloe vera juice 1.0 ml/kg body weight and isoniazid drug (LD) 50ml/kg body weight. Blood samples from each group were taken after 30th day through cardiac puncture for estimation of liver function test. The extent of liver damage was assessed by quantitative estimation of serum alanine aminotransferase (serum ALT), serum aspartate aminotransferase (serum AST), serum alkaline phosphatase (serum ALP), serum acid phosphatase (serum ACP), total serum protein (albumin and globulin) and serum bilirubin. Our investigations showed that the level of bilirubin was found to be higher in aloe vera juice and isoniazid drug administered groups. The level of serum ALT was found to be highest in rat fed with isoniazid drug, whereas the level of serum AST was found lowest in rats administered with isoniazid drug. The levels of serum ALP and serum ACP were found higher in concentration in rats administered with isoniazid drug. While the level of total proteins (albumin and globulin) was found to be low in group administered with isoniazid drug. Histopathological assessment of liver revealed that the animal exposed to isoniazid drug alone showed multifocal mild degree periportal mononuclear cell infiltration. Histological lesions ranged from hepatocellar disintegration and vacuolation in the peri-central vein area to marked proliferation of the rough endoplasmic reticulum.
The remaining groups, however showed normal lobular pattern of liver.
Article Details
Article Details
Aloe vera, Hepatotoxicity, Isoniazid drug, Liver
Dagne, E., Bisrat, D., Viljoen, A. and Vanwyk, B. E. (2000). Chemistry of aloe species. Curr. Org. Chem., 1055-1078
Dhuley G. P. and Naik, S. R. (1997). Protective effect of Rhinax, a herbal formulation against CCl4 induced liver injury and survival in rats. J. Ethnopharmacol., 56: 159-164
Friedman, Scott, Grendell, E., James, H., McQuaid and Kenneth, R. (2003). Current diagnosis and treatment in gastroenterology. New York .Lang Medical books / McGraw- Hill. pp. 664-679.
Karthikeyan, S. (2004). Hepatotoxicity of isoniazid: A study on the activity of marker enzymes of liver toxicity in serum and liver tissue of rabbits, Indian J.Pharmacol., 36 (4): 247-249.
Latha, U., Rajesh, M.G. and Latha, M.S. (1999). Hepatoprotective effect of an ayurvedic medicine. Indian drugs,36: 470- 473
Mitchell, J.R., Long, M.W., Thoregeirsson, U.P. and Jollow, D.J. (1974). Acetylation rates and monthly liver function tests during one year of isoniazid preventive therapy. Chest. 68: 181-190.
Mitchell, J.R., Zimmerman, H.J.,Ishak, K.G.,Thorgeirsson, U.P.,Timbrell, J.A. and Snodgrass, W.R. (1976). Isoniazid liver injury: Clinical spectrum, Pathology and probable pathogenesis. Ann. Intern. Med., 84:181-92.
Mitra, S.K. and Seshadri, S.J. (2000). Effect of HD-03-a herbal formulation in galactosamine-induced hepatopathy in rats. Indian J. Physiol. Pharmacy, 44:82-86.
Ni, Y. and Tizard, I.R. (2004). Analytical methodology: The gel analysis of aloe.pulp and its derivatives. In aloes. The genus Aloe; Reynolds, T., ED.; CRC Press: Bock Raton, pp 111-126
Ni, Y., Turner,D., Yates, K. and Tizard, I. (2004). Isolation and characterization of structural components of Aloe vera L. leaf pulp. Int. Immunopharmacol, 4: 1745-1755
Parthasarathy, R., Sarma, G. R., Janardhanam, B., Ramachandran, P., Santha, T. and Sivasubramanian, S. (1986). Hepatic toxicity in South Indian patients during treatment of tuberculosis with short-course regimens containing isoniazid, rifampicin and pyrazinamide. Tubercle, 67 : 99-108.
Rao, R.R. (1973). Mechanism of drug induced hepatotoxicity. I.J.Pharmacol., 5: 313-318.
Shakun, N.P. and Tabachuk, O.P. (1992). The comparative action of isoniazide,rifampicin and ethambutol on liver function. EKSP.Klin. Farmakol., 55 : 45.
Singh, .B, Saxena, A.K., Chandan, B.K., Anand, K.K., Suri, O.P., Suri, K.A. and Satti, N.K. (1998). Hepatoprotective activity of verbenalin on experimental liver damage in rodents. Fitoterapia., 69: 135-140.
Snodgrass, W., Potter, W. Z., Timbrell, J. A., Jollow, D. J. and Mitchell, J.R. (1974). Possible mechanism of isoniazid related hepatic injury. Clin. Res., 22: 323.
Sodhi, C.P., Rana, S.,Mehta, S., Vaiphe, K., Altari, S. and Mehta S. (1997). Study of oxidative stree in rifampicin-Induced hepatic injury in young rats. Drug Chem. Toxicol., 20: 255-69.
Venkateswaran, S., Pari, L., Viswanathan, P. and Menon, V.P. (1997). Protective effect a herbal formulation against erythromycin estolate -induced hepatotoxicity in rats. J. Ethnopharmacol., 57:161-167.
Vinson, J.A., Al Kharrat, H. and Andreoli, L.(2005). Effect of Aloe vera preparations on the human bioavailability of vitamins C and E. Phytomedicine, 12:760-765.
Wolf, P.L. (1999). Biochemical diagniosis of liver diseases. Indian J. Cli. Biochem., 14:59-90.
Yamamoto, I. (1973). Aloe ulcin, a new principle of Cape aloe and gastrointestinal function, especially experimental ulcer in rats. J. of the Medical Society of Toho University. 17:243-347.
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