Genotoxicity evaluation of pesticide profenofos by applying dominant lethal test on Culex quinquefasciatus
Article Main
Abstract
The present paper deals with the genotoxicity evaluation of profenofos by applying dominant lethal test (DLT) on Culex quinquefasciatus taken as an experimental model. For this, the males hatched from the larvae treated with LC20 of pesticide were crossmated with normal females and the results were based on the number of hatched and unhatched eggs laid by these females. Mean percentage frequency of unhatched eggs was as low as 3.97 in the normal stocks as compared to treated stocks in which the frequency of unhatched eggs had increased
to 9.50. The statistical analysis of the data from treated groups gave values of 9.50±1.35 as against 3.97± 0.38 in the control groups. Profenofos induced significant (p<0.05) dominant lethality.
Article Details
Article Details
Keywords
Profenofos, Dominant lethal test, Cx. quinquefasciatus
References
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Atienzar, F.A. and Jha, A.N. (2006). The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: A critical review. Mutat. Res., 613(2-3) : 76-102.
Bender, M.A., Bedford, J.S. and Mitchell, J.B. (1973). Mechanisms of chromosome aberration production II. Aberrations induced by 5-bromodeoxyuridine and visible light. Mutat. Res., 20 : 403-416.
Bender, M.A., Griggs, H.G. and Bedford, J.S. (1974). Mechanism of chromosomal aberration production III. Chemicals and ionizing radiation. Mutat. Res., 23 : 197-212.
Chaudhry, A. and Anand, P.K. (2004). Assessment of dominant lethal effects of chlorpyrifos (CPF) using mosquito genetics. Poll. Res., 23 (4) : 767-771.
Chaudhry, A., Barna, B. and Sharma, M. (2007). rDNA ITS 2 sequence based genotoxicity evaluation of imidacloprid using mosquito genome (Culicidae: Diptera). J. Cytol. Genet., 8 : 85-92.
Chaudhry, A., Bansal, M. and Kaura, T. (2009). Dominant lethal test based genotoxicity evaluation of glyphosate in Culex quinquefasciatus. J. Cytol. Genet., 11: 23-30.
Chaudhry, A. and Bhinder, P. (2009). Cypermethrin induced mutations in rDNA internal transcribed spacers 1 and 2 of Culex quinquefasciatus (Diptera: Culicidae). J. Appl. Biosci., 35 (1) : 7-12.
Chaudhry, A. and Lovleen (2008). Evaluation of mutagenic potential of carbaryl by dominant lethal test on Culex quinquefasciatus. J. Cytol. Genet., 9(N.S): 37-44.
Chaudhry, A. and Lovleen (2010). Chromosomal aberrations and dominant lethal test based genotoxicity assessment of dicofol by using mosquito genome. J. Cytol. Genet., 11: 11-20.
Clements, A.N. (1996). The biology of mosquitoes. London: Chapman and Hall.
Crumpton, T.L., Seidler, F.J. and Slotkin, T.A. (2000). Developmental neurotoxicity of chlorpyrifos in vivo and in vitro: effects on nuclear transcription factors involved in cell replication and differentiation. Brain Res., 857 (1-2): 87-98.
De Wolf, H., Blust, R. and Backeljau, T. (2004). The use of RAPD in ecotoxicology. Mutat. Res., 556: 249-262.
Evan, H.S. (1977). Molecular mechanism in the induction of chromosome aberrations. In: D. Scott, B.A. Bridges and F.H. Sobels (Eds.). Progress in Genetic Toxicology (pp 57-74), Amsterdam: Elseviser/North Holland Biomedical Press.
Fahmy, M.A. and Abdalla, E.F. (1998). Genotoxicity evaluation of buprofezin, petroleum oil and profenofos in somatic and germ cells of male mice. J. Appl. Toxicol., 18(5): 301-305.
Farrag, A.R.H. and Shalby, S.E.M. (2007). Comparative histopathological and histochemical studies on IGR, Lufenuron and Profenofos insecticide albino rats. J. Appl. Sci. Res., 3 (5): 377-386.
Finney, D.J. (1971). Probit analysis. Cambridge: Cambridge University Press.
Gaulden, M.E. and Liang, J.C. (1982). Insect cells for testing clastogenic agents. In: T.C. Hsu (Ed.). Cytogenetic Assays of Environmental Mutagens (pp107-135), NJ: Allanheld, Osmum.
Kavitha, P. and Venkateswara Rao, J. (2009). Sub-lethal effects of profenofos on tissue-specific antioxidative responses in a Euryhyaline fish, Oreochromis mossambicus. Ecotoxicol. Environ. Saf., 72: 1727–1733.
Krishnan, K.S. (1964). A note on colonization of Culex. Bull. WHO, 31: 455-456.
Li, X., Li, S., Liu, S. and Zhu, G. (2010). Lethal effect and in vivo genotoxicity of profenofos to Chinese native amphibian (Rana spinosa) tadpoles. Arch. Environ. Contam. Toxicol., 59: 478-483.
Loomis, T.A. and Hayes, A.W. (1996). Essentials of toxicology. 4th ed. London: Academic Press.
Manna, G.K. and Sarkar, C.S. (1998). Mutagenic potential of the antifungal antibiotic grisefulvin to orally administered experimental mice and its follow-up in F1 and F2 generations. Perspectives in Cytology and Genetics. AICCG-publi., Kalyani univ., Kalyani, India. p.383-398.
Moustafa, G.G., Ibrahim, Z.S, Hashimoto, Y., Alkelch, A.M., Sakamoto, K.Q., Ishizuka, M. and Fujita, S. (2007). Testicular toxicity of profenofos in matured male rats. Arch. Toxicol., 81(12): 875-81.
Muro, L.A. and Goyer, R.A. (1969). Chromosome damage in experimental lead poisoning. Arch. Pathol., 87: 660-663.
Naravaneni, R., Suman, G. and Jamil, K. (2006). In vitro cytogenetic studies of cypermethrin on human lymphocytes. Indian J. Exp. Biol., 44(3): 233-239.
Reddy, N.C. and Venkateswara Rao, J. (2008). Biological response of earthworm, Eisenia foetida (Savigny) to an organophosphorous pesticide, profenofos. Ecotoxicol. Environ. Saf., 71: 574–582.
Sharma, G.P., Sobti, R.C., Chaudhry, A. and Ahluwalia, K.K. (1989). Chromosomal aberrations and dominant lethal in Culex fatigans due to mercuric chloride. Cytobios, 59: 131-135.
Singh, K.R.P., Patterson, R.S., La-Brecque, G.C. and Razdan, R.K. (1975). Mass rearing of Culex pipiens fatigans Weid. J. Com. Dis., 7: 31-53.
Suter, K.E. (1975). Studies on the dominant lethal and fertility effects of the heavy metal compounds methylemercuric hydroxide, mercuric chloride and cadmium chloride in the male and female mice. Mutat. Res., 30: 365-374.
Venkateswara Rao, J., Begum, G., Jakka, N.M., Srikanth, K. and Nageswara Rao, R. (2006). Sublethal effects of profenofos on locomotor behavior and gill architecture of the mosquito fish, Gambusia affinis. Drug Chem. Toxicol., 29 (3) : 255-267.
Venkateswara Rao, J., Shilpanjali, D., Kavitha, P. and Madhavendra, S.S. (2003). Toxic effects of profenofos on tissue acetylcholinesterase and gill morphology in a euryhaline fish, Oreochromis mossambicus. Arch. Toxicol., 77(4): 227-232.
Atienzar, F.A. and Jha, A.N. (2006). The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: A critical review. Mutat. Res., 613(2-3) : 76-102.
Bender, M.A., Bedford, J.S. and Mitchell, J.B. (1973). Mechanisms of chromosome aberration production II. Aberrations induced by 5-bromodeoxyuridine and visible light. Mutat. Res., 20 : 403-416.
Bender, M.A., Griggs, H.G. and Bedford, J.S. (1974). Mechanism of chromosomal aberration production III. Chemicals and ionizing radiation. Mutat. Res., 23 : 197-212.
Chaudhry, A. and Anand, P.K. (2004). Assessment of dominant lethal effects of chlorpyrifos (CPF) using mosquito genetics. Poll. Res., 23 (4) : 767-771.
Chaudhry, A., Barna, B. and Sharma, M. (2007). rDNA ITS 2 sequence based genotoxicity evaluation of imidacloprid using mosquito genome (Culicidae: Diptera). J. Cytol. Genet., 8 : 85-92.
Chaudhry, A., Bansal, M. and Kaura, T. (2009). Dominant lethal test based genotoxicity evaluation of glyphosate in Culex quinquefasciatus. J. Cytol. Genet., 11: 23-30.
Chaudhry, A. and Bhinder, P. (2009). Cypermethrin induced mutations in rDNA internal transcribed spacers 1 and 2 of Culex quinquefasciatus (Diptera: Culicidae). J. Appl. Biosci., 35 (1) : 7-12.
Chaudhry, A. and Lovleen (2008). Evaluation of mutagenic potential of carbaryl by dominant lethal test on Culex quinquefasciatus. J. Cytol. Genet., 9(N.S): 37-44.
Chaudhry, A. and Lovleen (2010). Chromosomal aberrations and dominant lethal test based genotoxicity assessment of dicofol by using mosquito genome. J. Cytol. Genet., 11: 11-20.
Clements, A.N. (1996). The biology of mosquitoes. London: Chapman and Hall.
Crumpton, T.L., Seidler, F.J. and Slotkin, T.A. (2000). Developmental neurotoxicity of chlorpyrifos in vivo and in vitro: effects on nuclear transcription factors involved in cell replication and differentiation. Brain Res., 857 (1-2): 87-98.
De Wolf, H., Blust, R. and Backeljau, T. (2004). The use of RAPD in ecotoxicology. Mutat. Res., 556: 249-262.
Evan, H.S. (1977). Molecular mechanism in the induction of chromosome aberrations. In: D. Scott, B.A. Bridges and F.H. Sobels (Eds.). Progress in Genetic Toxicology (pp 57-74), Amsterdam: Elseviser/North Holland Biomedical Press.
Fahmy, M.A. and Abdalla, E.F. (1998). Genotoxicity evaluation of buprofezin, petroleum oil and profenofos in somatic and germ cells of male mice. J. Appl. Toxicol., 18(5): 301-305.
Farrag, A.R.H. and Shalby, S.E.M. (2007). Comparative histopathological and histochemical studies on IGR, Lufenuron and Profenofos insecticide albino rats. J. Appl. Sci. Res., 3 (5): 377-386.
Finney, D.J. (1971). Probit analysis. Cambridge: Cambridge University Press.
Gaulden, M.E. and Liang, J.C. (1982). Insect cells for testing clastogenic agents. In: T.C. Hsu (Ed.). Cytogenetic Assays of Environmental Mutagens (pp107-135), NJ: Allanheld, Osmum.
Kavitha, P. and Venkateswara Rao, J. (2009). Sub-lethal effects of profenofos on tissue-specific antioxidative responses in a Euryhyaline fish, Oreochromis mossambicus. Ecotoxicol. Environ. Saf., 72: 1727–1733.
Krishnan, K.S. (1964). A note on colonization of Culex. Bull. WHO, 31: 455-456.
Li, X., Li, S., Liu, S. and Zhu, G. (2010). Lethal effect and in vivo genotoxicity of profenofos to Chinese native amphibian (Rana spinosa) tadpoles. Arch. Environ. Contam. Toxicol., 59: 478-483.
Loomis, T.A. and Hayes, A.W. (1996). Essentials of toxicology. 4th ed. London: Academic Press.
Manna, G.K. and Sarkar, C.S. (1998). Mutagenic potential of the antifungal antibiotic grisefulvin to orally administered experimental mice and its follow-up in F1 and F2 generations. Perspectives in Cytology and Genetics. AICCG-publi., Kalyani univ., Kalyani, India. p.383-398.
Moustafa, G.G., Ibrahim, Z.S, Hashimoto, Y., Alkelch, A.M., Sakamoto, K.Q., Ishizuka, M. and Fujita, S. (2007). Testicular toxicity of profenofos in matured male rats. Arch. Toxicol., 81(12): 875-81.
Muro, L.A. and Goyer, R.A. (1969). Chromosome damage in experimental lead poisoning. Arch. Pathol., 87: 660-663.
Naravaneni, R., Suman, G. and Jamil, K. (2006). In vitro cytogenetic studies of cypermethrin on human lymphocytes. Indian J. Exp. Biol., 44(3): 233-239.
Reddy, N.C. and Venkateswara Rao, J. (2008). Biological response of earthworm, Eisenia foetida (Savigny) to an organophosphorous pesticide, profenofos. Ecotoxicol. Environ. Saf., 71: 574–582.
Sharma, G.P., Sobti, R.C., Chaudhry, A. and Ahluwalia, K.K. (1989). Chromosomal aberrations and dominant lethal in Culex fatigans due to mercuric chloride. Cytobios, 59: 131-135.
Singh, K.R.P., Patterson, R.S., La-Brecque, G.C. and Razdan, R.K. (1975). Mass rearing of Culex pipiens fatigans Weid. J. Com. Dis., 7: 31-53.
Suter, K.E. (1975). Studies on the dominant lethal and fertility effects of the heavy metal compounds methylemercuric hydroxide, mercuric chloride and cadmium chloride in the male and female mice. Mutat. Res., 30: 365-374.
Venkateswara Rao, J., Begum, G., Jakka, N.M., Srikanth, K. and Nageswara Rao, R. (2006). Sublethal effects of profenofos on locomotor behavior and gill architecture of the mosquito fish, Gambusia affinis. Drug Chem. Toxicol., 29 (3) : 255-267.
Venkateswara Rao, J., Shilpanjali, D., Kavitha, P. and Madhavendra, S.S. (2003). Toxic effects of profenofos on tissue acetylcholinesterase and gill morphology in a euryhaline fish, Oreochromis mossambicus. Arch. Toxicol., 77(4): 227-232.
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Genotoxicity evaluation of pesticide profenofos by applying dominant lethal test on Culex quinquefasciatus. (2011). Journal of Applied and Natural Science, 3(2), 224-227. https://doi.org/10.31018/jans.v3i2.183
