Effect of different fractions of Hibiscus rosa sinensis leaf extract on islets of Langerhans and antioxidant activity in non-obese diabetic (NOD) mouse
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Abstract
The present investigation was undertaken to study the effect of Hibiscus rosa sinensis leaf extract fractions on islets of Langerhans, total antioxidant activity in non-obese diabetic (NOD) mouse. Two sets of experiments were conducted. First experiment was designed to test the hypoglycemic properties of different isolated fractions. Five fractions were screened for hypoglycemic property. Fractions F3, F4 and F5 showed hypoglycemic in NOD mice. Second set of experiment was conducted to assess the effects of above fractions on the diameter of islets of Langerhans and on the â-cells number and total antioxidant capacity (TAC). All the fractions had a significant effect in the enhancement of diameter of islets of Langerhans and the â-cell number and antioxidant active (total antioxidant capacity) compared with control NOD mice.
Article Details
Article Details
Keywords
Blood glucose, Islets of langerhans, Total antioxidant capacity, Non-obese diabetic mice
References
Abuaisha, B., Kumar, S. and Malik, R. (1998). Relationship of elevated urinary albumin excretion to components of the metabolic syndrome in non-insulin-dependent diabetes mellitus. Diabetes Res clin pract., 39: 93-99.
Acha-Orbea, H. and McDevitt, H. O. (1990). The role of class II molecules in development of insulin-dependent diabetes mellitus in mice, rats and humans. Curr. Top. Microbiol. Immunol., 156: 103-119.
Alberti, K.G.M.M., Zimmet, P. and DeFronzo, R.A. (1997). International Textbook Diabetes Mellitus, 2nd ed. John Wiley and Sons, New York.
Berker, K. I., Guclu, K., Top, I., Demirata, B. and Apak, R. (2009). Total antioxidant capacity Assay Using Optimized Ferricyanide/Prussian Blue Method. Food Anal. Methods. DOI 10. 1007/s 12161-9117-9.
Cahill, GF. and McDevitt, H.O. (1981). Insulin-dependent diabetes mellitus: the initial lesion. N. Engl. J. Med., 304 : 1454-1465.
Castano, L. and Eisenbarth, G. S. (1990). Type-I diabetes: a chronic autoimmune disease of human, mouse, and rat. Annu. Rev. Immunol., 8: 647-679.
Chandavar, V. R. and Naik, P. R. (2004).Variation in plasma glucose and pancreatic â-cell in the turtle Lissemys punctata (order: Chelonia; Family: Trionycnidae). Acta Zoologica (Stockholm), 85: 113-118.
Dor, Y., Brown, J., Martinez, Ol. and Melton, DA. (2004). Adult pancreatic â-cells are formed by self-duplication rather than stem cell differentiation. Nature, 429: 41-46.
Ganong, W. (1999). Review of medical physiology. Stamford, Connecticut, Appleton and Lange, 324-328.
Gauthaman, K. K., Saleem, M. T., Thanislas, P. T., Prabhu, V. V., Krishnamoorthy, K. K., Devaraj, N. S. and Somasundaram, J. S. (2006). Cardioprotective effect of the Hibiscus rosa-sinensis flowers in an oxidative stress model of myocardial ischemic reperfusion injury in rat. BMC Complementary and Alternative Medicine, 6:32.
Gorsuch, A. N., Spencer, K. M., Lister, J., McNally, J.M., Dean, B.M., Tottazzo, G. F. and Cudworth, A. G. (1981). Evidence for a long prediabetic period in type 1 insulindependent) diabetes mellitus. Lancet, 2:1363-1365,
Makino, S., Kunimoto, K., Muraoka, Y., Mizushima, Y., Katagiri, K. and Tochino, Y. (1980). Breeding of a nonobese diabetic strain of mice. Jikken Dobutsu., 29: 1-13.
Masaki, H. S., Sakaki, S., Atsumi, T. and Sakurai, H. (1995). Active oxygen scavenging activity of plant extracts. Biol. Pharmacol. Bull., 18: 162-166.
Moqbel, F.S., Naik, P.R., Najma, H.M. and Selvaraj, S. (2011). Antidiabetic properties of Hibiscus rosa sinensis L. leaf extract fraction on non-obese diabetic (NOD) mouse. Ind. J. Exp. Biol., 49 : 24-29.
Nazirog¡lu, M. and Butterworth, P. (2005). Protective effects of moderate exercise with dietary vitamin C and E on blood antioxidative defense mechanism in rats with streptozotocin induced diabetes. Can J Appl Physiol., 30 :172-185.
Obi, F. O. and Uneh, E, PH. (2003). dependant prevention of carbontetrachloride-induce lipoperoxidation in rats by ethanolic extract of Hibiscus rosa-sinensis petals. Biokemistri, 13 : 42-50.
Packer, L., Rfsen, P., Tritschler, H.J., King, G.L. and Azzi, A. (2000). Antioxidants diabetes management. Marcel Dekker, New York, pp. 1–338.
Philippe, J. (1991). Structure and pancreatic expression of the insulin and glucagon genes. Endocrine Reviews. 12 : 252-271.
Polidori, M.C., Mecocci, P., Stahl, W, Parente, B., Cecchetti, R., Cherubini, A., Cao P., Sies, H. and Senin, U. (2000). Plasma levels of lipophilic antioxidants in very old patients with type II diabetes. Diabetes Metab Res Rev, 16:15-19.
Rahbani-Nobar, M. E., Rahimi-pour, A., Rahbani-Nobar, M., Adi-Beig and Mirhashemi, S. M. (1999). Total antioxidant capacity, superoxide dismutase and glutathion peroxidase in diabetic patients. Medical Journal of Islamic Academy of Sciences, 12: 109-114.
Rossini, A., Mordes, J. P. and Greiner, D. L. (1989). The pathogenesis of autoimmune diabetes mellitus. Curr. Opinion Immunol., 2: 598-603.
Sachdewa, A. and Khemani, L. D. (2003). Effect of Hibiscus rosa-sinensis Linn. ethanol flower extract on blood glucose and lipid profile in streptozotocin induced diabetes in rats. Journal of Ethnopharmacology, 89: 61–66.
Sharma, S., Khan, N. and Sultana, S. (2004). Study on prevention of twostage skin carcinogenesis by Hibiscus rosa sinensis extract and the role of its chemical constituent, gentisic acid,
in the inhibition of tumor promotion response and oxidative stress in mice. Euro J Cancer Prevent, 13: 53-63.
Shinn, S.H. (1998). Oxidative stress and diabetic vascular complications. In: Recent advances on pathogenesis and management of diabetes mellitus. 1st ed, Elsevier Science Co, Singapore, pp 3-8.
Acha-Orbea, H. and McDevitt, H. O. (1990). The role of class II molecules in development of insulin-dependent diabetes mellitus in mice, rats and humans. Curr. Top. Microbiol. Immunol., 156: 103-119.
Alberti, K.G.M.M., Zimmet, P. and DeFronzo, R.A. (1997). International Textbook Diabetes Mellitus, 2nd ed. John Wiley and Sons, New York.
Berker, K. I., Guclu, K., Top, I., Demirata, B. and Apak, R. (2009). Total antioxidant capacity Assay Using Optimized Ferricyanide/Prussian Blue Method. Food Anal. Methods. DOI 10. 1007/s 12161-9117-9.
Cahill, GF. and McDevitt, H.O. (1981). Insulin-dependent diabetes mellitus: the initial lesion. N. Engl. J. Med., 304 : 1454-1465.
Castano, L. and Eisenbarth, G. S. (1990). Type-I diabetes: a chronic autoimmune disease of human, mouse, and rat. Annu. Rev. Immunol., 8: 647-679.
Chandavar, V. R. and Naik, P. R. (2004).Variation in plasma glucose and pancreatic â-cell in the turtle Lissemys punctata (order: Chelonia; Family: Trionycnidae). Acta Zoologica (Stockholm), 85: 113-118.
Dor, Y., Brown, J., Martinez, Ol. and Melton, DA. (2004). Adult pancreatic â-cells are formed by self-duplication rather than stem cell differentiation. Nature, 429: 41-46.
Ganong, W. (1999). Review of medical physiology. Stamford, Connecticut, Appleton and Lange, 324-328.
Gauthaman, K. K., Saleem, M. T., Thanislas, P. T., Prabhu, V. V., Krishnamoorthy, K. K., Devaraj, N. S. and Somasundaram, J. S. (2006). Cardioprotective effect of the Hibiscus rosa-sinensis flowers in an oxidative stress model of myocardial ischemic reperfusion injury in rat. BMC Complementary and Alternative Medicine, 6:32.
Gorsuch, A. N., Spencer, K. M., Lister, J., McNally, J.M., Dean, B.M., Tottazzo, G. F. and Cudworth, A. G. (1981). Evidence for a long prediabetic period in type 1 insulindependent) diabetes mellitus. Lancet, 2:1363-1365,
Makino, S., Kunimoto, K., Muraoka, Y., Mizushima, Y., Katagiri, K. and Tochino, Y. (1980). Breeding of a nonobese diabetic strain of mice. Jikken Dobutsu., 29: 1-13.
Masaki, H. S., Sakaki, S., Atsumi, T. and Sakurai, H. (1995). Active oxygen scavenging activity of plant extracts. Biol. Pharmacol. Bull., 18: 162-166.
Moqbel, F.S., Naik, P.R., Najma, H.M. and Selvaraj, S. (2011). Antidiabetic properties of Hibiscus rosa sinensis L. leaf extract fraction on non-obese diabetic (NOD) mouse. Ind. J. Exp. Biol., 49 : 24-29.
Nazirog¡lu, M. and Butterworth, P. (2005). Protective effects of moderate exercise with dietary vitamin C and E on blood antioxidative defense mechanism in rats with streptozotocin induced diabetes. Can J Appl Physiol., 30 :172-185.
Obi, F. O. and Uneh, E, PH. (2003). dependant prevention of carbontetrachloride-induce lipoperoxidation in rats by ethanolic extract of Hibiscus rosa-sinensis petals. Biokemistri, 13 : 42-50.
Packer, L., Rfsen, P., Tritschler, H.J., King, G.L. and Azzi, A. (2000). Antioxidants diabetes management. Marcel Dekker, New York, pp. 1–338.
Philippe, J. (1991). Structure and pancreatic expression of the insulin and glucagon genes. Endocrine Reviews. 12 : 252-271.
Polidori, M.C., Mecocci, P., Stahl, W, Parente, B., Cecchetti, R., Cherubini, A., Cao P., Sies, H. and Senin, U. (2000). Plasma levels of lipophilic antioxidants in very old patients with type II diabetes. Diabetes Metab Res Rev, 16:15-19.
Rahbani-Nobar, M. E., Rahimi-pour, A., Rahbani-Nobar, M., Adi-Beig and Mirhashemi, S. M. (1999). Total antioxidant capacity, superoxide dismutase and glutathion peroxidase in diabetic patients. Medical Journal of Islamic Academy of Sciences, 12: 109-114.
Rossini, A., Mordes, J. P. and Greiner, D. L. (1989). The pathogenesis of autoimmune diabetes mellitus. Curr. Opinion Immunol., 2: 598-603.
Sachdewa, A. and Khemani, L. D. (2003). Effect of Hibiscus rosa-sinensis Linn. ethanol flower extract on blood glucose and lipid profile in streptozotocin induced diabetes in rats. Journal of Ethnopharmacology, 89: 61–66.
Sharma, S., Khan, N. and Sultana, S. (2004). Study on prevention of twostage skin carcinogenesis by Hibiscus rosa sinensis extract and the role of its chemical constituent, gentisic acid,
in the inhibition of tumor promotion response and oxidative stress in mice. Euro J Cancer Prevent, 13: 53-63.
Shinn, S.H. (1998). Oxidative stress and diabetic vascular complications. In: Recent advances on pathogenesis and management of diabetes mellitus. 1st ed, Elsevier Science Co, Singapore, pp 3-8.
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Effect of different fractions of Hibiscus rosa sinensis leaf extract on islets of Langerhans and antioxidant activity in non-obese diabetic (NOD) mouse. (2011). Journal of Applied and Natural Science, 3(2), 206-210. https://doi.org/10.31018/jans.v3i2.180
