Isolation of newer probiotic microorganisms from unconventional sources
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Abstract
Probiotics are live microbes in the form of dried or fermented cells that are highly beneficial for human health. The food industry has been revolutionised due to introduction of potential probiotic organisms in a varied formulations derived from mostly dairy products. Isolation of probiotic microbes from unexplored non-dairy sources is gaining attention these days. In the present study, six potential probiotic isolates from non-dairy sources were obtained that are also biocompatible with each other. Out of these, five isolates were gram positive rod shaped and one was gram negative rod shaped. These isolates were able to grow in presence of lysozyme, low pH and bile salts with good adherence ability. The market for probiotic microorganisms from unconventional products is accelerating to deal with lactose intolerant people. These probiotic attribute studies revealed their potential to be exploited at industrial scale.
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Keywords
Antimicrobial activity, bile tolerance, fermented cells, hydrophobicity, probiotics
References
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Tokat, M. Gulgor, G. Elmac, S.B. Isleyen, N.A. and Ozcelik, F. (2015). In Vitro properties of potential probiotic indigenous lactic acid bacteria originating from traditional pickles. BioMed Res. Int. Volume 2015: Article ID 315819, 8 pages.
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Vasiljevic, T. and Shah, N.P. (2008). Review: Probiotics-from metchnikoff to bioactives. Int. Dairy J. 18:714-728.
Voges, D. and Proskauer, B. (1898) Beitrag zur Ernahrungsphysiologie and zur Differential diagnose der Bakterien der hamorrhagischen Septicemia. Z. Hyg. 28, 20-32.
Christensen, W. B. (1946). Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. J. Bacteriol. 52: 461-466.
Colbey, C. Cox, A.J. Pyne, D.B. Zhang, P. Cripps, A.W. and West, N.P. (2018). Upper respiratory symptoms, gut health and mucosal immunity in athletes. Sports Med. 48: 65-77.
Collado, M.C. Meriluoto, J. and Salminen, S. (2007). Role of commercial probiotic strains against human pathogen adhesion to intestinal mucus. Lett. Appl. Microbiol. 45: 454-460.
Das, D. and Goyal, A. (2014). Characterization of a non-cytotoxic bacteriocin from probiotic Lactobacillus plantarum DM5 with potential as a food preservative. Food Funct. 5: 2453-2462.
Ezendam, J. and Loveren, H. (2006). Probiotics: Immunomodulation and evaluation of safety and efficacy. Nutr. Rev. 64:1-14.
Isenberg H. D. and Sundheim, L. H. (1958) Indole reactions in bacteria. J. Bacteriol. 75, 682–690.
Markowiak, P. and Slizewska, K. (2017). Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients 9:1021.
Merenstein, D. and Salminen, S. (2017). Probiotics and prebiotics.
Munoz-Quezada, S. Chenoll, E. Vieites, J.M., Genoves, S. Maldonado, J. Bermudez-Brito, M. Gomez-Llorente, C. Matencio, E. Bernal, M.J. Romero, F. Suarez, A. Ramon, D. and Gil, A. (2013). Isolation, identification and characterization of three novel probiotic strains (Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036) from the faeces of exclusively breast-fed Infants. Br. J. Nutr. 109:S51-S62.
O’Hara, A.M. and Shanahan, F. (2007). Gut microbiota: Mining for therapeutic potential. Clin. Gastroenterol. Hepatol. 5:274-284.
Parvez, S. Malik, K.A. Kang, K.A. and Kim, H.Y. (2006). Probiotics and their fermented food products are beneficial for health: Review. J. Appl. Microbiol. 100:1171-1185.
Prawan, K. and Bhima, B. (2017). Isolation and characterization of lactic acid bacteria for probiotic application from plant sources. Int. J. Adv. Res. 5(4): 869-876.
Sanders, M.E. Morelli, L. and Tompkins, T.A. (2003). Spore formers as human probiotics: Bacillus, Sporolactobacillus and Brevibacillus. Compr. Rev. Food Sci. Food Safety. 2:101-110.
Saran, S. Bisht, M.S. Singh, K. and Teotia, U.S. (2012). Analyzing probiotic attributes to assess comparatively two isolates of Lactobacillus acidophilus in prebiotics, honey and inulin. DHR IJBLS. 2: 26-34.
Shokryazdan, P. Sieo, C.C. Kalavathy, R. Liang, J.B. Alitheen, N.B. Jahromi, M.F. and Ho, Y.W. (2014). Probiotic potential of Lactobacillus Strains with antimicrobial activity against some human pathogenic strains. BioMed Res. Int. Volume 2014: Article ID 927268, 16 pages.
Simmons, J.S. (1926). A culture media for differentiating organisms of typhoid colon aerogenes groups and for isolation of certain fungi. J. Infect. Dis. 39:209.
Sornplang, P. and Piyadeatsoontorn, S. (2016). Probiotic isolates from unconventional sources: A Review. J. Ani. Sci. Technol. 58:26.
Taylor, W. I. and Achanzar, D. (1972). Catalase test as an aid to the identification of Enterobacteriaceae. Appl. Microbiol. 24: 58-61.
Tokat, M. Gulgor, G. Elmac, S.B. Isleyen, N.A. and Ozcelik, F. (2015). In Vitro properties of potential probiotic indigenous lactic acid bacteria originating from traditional pickles. BioMed Res. Int. Volume 2015: Article ID 315819, 8 pages.
Trikha, R. Sharma, A. Guglani, V. Rishi, P. and Tewari, R. (2015). Isolation of probiotic Lactobacilli from human infants stool samples exhibiting antimicrobial activity against pathogenic microorganisms. J. Pure Appl. Microbiol. 9(1):377-390.
Valgas, C. De-Souza, S.M. Smania, E.F. and Smania, Jr A. (2007). Screening methods to determine antibacterial activity of natural products. Braz. J. Microbiol. 38:369-380.
Vasile, N. Ghindea, R. and Vassu, T. (2011). Probiotics-An alternative treatment for various diseases. Roum. Arch. Microbiol. Immunol. 70:54-59.
Vasiljevic, T. and Shah, N.P. (2008). Review: Probiotics-from metchnikoff to bioactives. Int. Dairy J. 18:714-728.
Voges, D. and Proskauer, B. (1898) Beitrag zur Ernahrungsphysiologie and zur Differential diagnose der Bakterien der hamorrhagischen Septicemia. Z. Hyg. 28, 20-32.
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How to Cite
Isolation of newer probiotic microorganisms from unconventional sources. (2018). Journal of Applied and Natural Science, 10(3), 847-852. https://doi.org/10.31018/jans.v10i3.1724
