Increase in intracellular proline content in Anabaena variabilis during stress conditions
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Abstract
The present study deals with the effects of stress due to varying concentrations of sodium chloride, cadmium, pH and exposure to pesticides on intracellular proline accumulation in Anabaena variabilis in laboratory conditions. The stresses induced due to these changes in their growth media were expressed as decrease in growth (by 40% at pH 5; 30% at 50 mM NaCl; 80% at 50 ?M endosulphan and 55% at 10 ?M CdSO4) as measured in terms of chlorophyll a concentrations in the test organism. Low pH (5, 6) had stronger negative effect on growth
than increasing alkaline pH. The cyanobacterium showed varying degree of susceptibility to increasing salt, cadmium and endosulphan concentrations. Under all these altered conditions, their intracellular proline concentration was found to increase linearly in the test organism with increase in its amount of stress inducing substances in its vicinity. Proline appears to be a stress provoked substance in A. variabilis. However, there seemed to be a breakdown in the proline synthesis mechanism when concentrations of such compounds reached toxic proportions.
Article Details
Article Details
Cyanobacteria, NaCl, Cadmium, pH, Endosulphan, Intracellular proline
Alia and Saradhi, P. P. (1991). Proline accumulation under heavy metal stress. J Plant Physiol., 138:554–558.
Alia, Mohanty, P. and Matysik, J. (2001). Effect of Proline on the production of singlet oxygen. Amino Acid, 21: 195-200.
Bates, L.S., Waldren, R.P. and Teare, I. D. (1973). Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-207.
Delauney, A. and Verma, D.P.S. (1993). Proline biosynthesis and osmoregulation in plants. Plant J., 4: 215-223.
Farago, M.E. and Mullen, W.A. (1979). Plants which accumulate metals. Part IV. A possible copper-Proline complex from the roots of Armeria maritima. Inorg Chim Acta, 32: 93-94.
Fatma, T., Khan, M.A. and Choudhary, M. (2007). Impact of environmental pollution on cyanobacterial proline content. J Appl Phycol., 19: 625-629.
Hanson, A.D. and Burnet, M. (1994). Evolution and metabolic engineering of osmoprotectant accumulation in higher plants. In: Cherry JH (ed) Biochemical and cellular mechanisms of stress tolerance in plants. Springer, Berlin, 291–302.
Hare, P.D., Cress, W.A. and Van Staden, J. (1998). Dissecting the roles of osmolyte accumulation during stress. Plant Cell Environ, 21:535–553.
Inabha, M., Sakamato, A. and Murata, N. (2001). Functional expression in E.coli of low affinity and high affinity Na+ (Li) +/H+ antiporters of Synechocystis. J. Bacteriol., 183: 1376-1384.
Jurgensen, M.F. and Davey, C.B. (1968). Nitrogen fixating blue-green algae in acid forest and nursery soils. Can. J. Microbiol., 14: 1179.
Leena, T. and Fatma, T. (2000). Potential use of Phormidium for bioremediation of copper. Ind. J. Appl. Pure Biol., 15: 83-86.
Mackinney, G. (1941). Absorption of light by chlorophyll solutions. J.Biol.Chem., 140: 315-322.
Mansour, M.M.F., Salama, K.H.A., Ali, F.Z.M. and Abou Hadid, A.F. (2005). Cell and plant responses to NaCl in Zea mays L. Cultivars differing in salt tolerance. Gen Appl Plant Physiol., 31:29–41.
Mehta, S. and Gaur, J.P. (1999). Heavy metal induced proline accumulation and its role in ameliorating metal toxicity in chlorella vulgaris. New Phytol., 143: 253-259.
Rippka, R., Dervelles, J., Waterbury, J.B., Herdman, M. and Stanier, R.Y. (1979). Genetic assignment, strain histories and properties of pure culture of cyanobacteria. J.Gen. Microbiol., 111: 1-61.
Rosko, J. J. and Rachlin, J. W. (1975). The effect of copper, zinc, cobalt and manganese on the growth of marine diatom Nitzchia closterium. Bull Torr Bot Club. 102:100–106.
Schubert, H., Fulda, S. and Hagemann, M. (1993). Effects of adaptation to different salt concentrations on photosynthesis and pigmentation of the cyanobacterium Synechocystis sp. PCC 6083. J Plant Physiol., 142:291-295.
Shah, K. and Dubey, R.S. (1998). Effect of Cadmium elevates the protein level and alters the activity of proteolytic enzymes in germinating rice seeds. Acta Physiologiae Plantarum., 20: 189-196.
Smirnoff, N. and Cumbes, Q.J. (1989). Hydroxyl radical scavenging activity of compatible solute. Phytochemistry, 28: 1057-1060.
Syiem, M.B., Nongbri, B.B., Pinokiyo, A., Bhattacharjee, A., Nongrum, N.A. and Hynniewta, L. (2010). Significance of cyanobacterial diversity in different ecological conditions of Meghalaya, India. J. Appl. & Nat. Sci., 2(1):134-139.
Watanabe, S., Kojima, K., Ide, Y., and Sasaki, S. (2000). Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. Plant Cell Tissue Organ Cult., 63:199–206.
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