Hyaluronic acid, is extract by different procedures from various sources like pig, rabbit, oxes and human are available, but these processes have certain imitations like low yield, and also it requires the killing of these animals which is against the experimental ethics. In the present study, we have carried out the extraction of hyaluronic acid from cockâ€™s comb which was further analyzed with qualitative test, viscosity, UV absorption, endotoxin detection assay. Also, the protein contamination of extracted hyaluronic acid was determined by using SDS-PAGE of hyaluronic acid was studied for checking the protein contaminants and it was noted that there were no bands observed in the well loaded with extracted hyaluronic acid sample indicating that the final extract of hyaluronic acid is not contaminated with the protein. The extraction and purification of hyaluronic acid by using the method reported here give pure hyaluronic acid. The viscosity of extracted hyaluronic acid was found to be 2.55 poise which is economical and can be used for industrial production of hyaluronic acid having clinical applications
Hyaluronic acid, Osteoarthritis, protein contamination Rooster combs, SDS-PAGE
Cassaro, C.M. and Dietrich, C.P. (1977). Distribution of sulfated mucopolysaccharides in invertebrates. J Biol Chem., 252(7):2254â€“2261.
Chester, T. Stafford, William, Niedermeier, Howard, Holley, L. and Ward Pigman (1964). Studies on the Concentration and Intrinsic Viscosity of Hyaluronic Acid in Synovial Fluids of Patients with Rheumatic Diseases Ann. rheum. dis. 23:152-157.
Csoka T.B., Frost, G.I., Stern, R. (1997). Hyaluronidases in tissue invasion. Invasion Metastasis. 17:297- 311.
Eiji Shimada and Go Matsumura. (1995). Viscosity and Molecular Weight of Hyaluronic Acids. J Biochem 78 (3): 513-517.
Lapcik, L., Ch. Dammer and M. Valko. (1992). Hyaluronic acid copper(II) complexes: spectroscopic characterization. Journal colloid and polymer science, 27(10):1049-1052.
Mason, R.M., d'Arville, C., Kimura JH, Hascall VC. (1982). Absence of covalently linked core protein from newly synthesized hyaluronate. Biochem J., 207(3):445â€“457.
Prehm P. (1983). Synthesis of hyaluronate in differentiated teratocarcinoma cells. Mechanism of chain growth. Biochem J., 211(1):191â€“198.
Ramalingam, K. and Ravindranath, M. H. (1970). Histochemical significance of green metachromasia to Toluidine blue. Histochemistry and cell biology. 24(4): 322-327.
Swann, D.A. (1968). Studies on hyaluronic acid. I. The preparation and properties of rooster comb hyaluronic acid. Biochim Biophys Acta., 156(1):17â€“30.
Swann, D.A. (1968). Studies on hyaluronic acid. II. The protein component(s) of rooster comb hyaluronic acid. Biochim Biophys Acta., 160 (1):96â€“105.
Weiss, C, Balazs, E.A., St. Onge, R., Denlinger, J.L. (1981). Clinical studies of the intra articular injection of HealonR (sodium hyaluronate) in the treatment of osteoarthritis of human knees. Osteoarthritis symposium. Palm Aire, Fla., 20-22.
West, D.C., Chen, H. (2000). Is hyaluronan degradation an angiogenic/ metastatic switch? New Frontiers in Medicinal Sciences: Redefining Hyaluronan; Elsevier Science, 77-86.
Balazs EA, Watson D, Duff IF, Saul R (1967) Hyaluronic acid in synovial fluid. I. Molecular parameters of hyaluronic acid in normal and arthritic human fluids. Arthritis Rheum, 10: 357-376.
Moreland LW (2003) Intra-articular hyaluroran and hylans for the treatment of osteoarthritis: mechanisms of action. Arthritis Res. Ther., 5: 54-67.
Goldberg, V.M., Buckwalter, J.A. (2005). Hyaluronans in the treatment of osteoarthritis of the knee: evidence for disease modifying activity. Osteoarthritis Cartilage, 13: 216- 224.
Pelletier, J.P., Martel-Pelletier, J. (1993). The pathophysiology of osteoarthritis and the implication of the use of hyaluron and hylan as therapeutic agents in viscosupplementation. J. Rheumatol Suppl., 39: 19-24.
He, F. (2011). Laemmli-SDS-PAGE. Bio-protocol Bio101: e80. DOI:10.21769/BioProtoc.80.
Meyer, K., Palmer, J. W. (1979). Extraction from rooster combs and purification: EP 138 572.
Prescott A. L. (2003). Method for purifying high molecular weight hyaluronic acid. USP 6660853.
This work is licensed under Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Â© Author (s)