The relative availabilities of nitrogen and sulphur can modulate seed storage protein composition in grain legumes and cereals. Soybean contains two major seed storage proteins, glycinins and β-conglycinins that account for approximately 70% of total protein and their composition is affected by nitrogen and sulphur supplies. The present study demonstrated the effect of sulphur (Gypsum @ 20 kg S ha-1 ) and recommended a dose of nitrogen (Urea @ 31.25 kg N ha-1 ) alone or in combination on accumulation patterns of various protein fractions and protein quality in soybean seeds under agro-climatic conditions of Punjab. Application of nitrogen or sulphur alone decreased the relative proportion of β-subunit of purified β-conglycinin fraction than control. The acidic polypeptides of glycinin fraction of globulin and 11S/7S ratio were increased in all the treatments in comparison to control, and the maximum increase was reported in the combined application of gypsum with the recommended dose of urea. The lower proportion of total 7S, increased acidic polypeptides and improved 11S:7S ratio by combined treatment of nitrogen and sulphur suggests that gypsum @ 20 kg S ha-1 can be beneficial when applied along the recommended nitrogen dose to improve soybean protein quality.
Nitrogen, Protein quality, Soybean, Sulphur
Arslanoglu, F., Aytac, S. and Oner, E.K. (2011). Effect of genotype and environment interaction on oil and protein content of soybean (Glycine max (L.) Merrill) seed. African J Biotechnol, 10(80): 18409-18417.
Awazuhara, M., Kim, H. and Hayashi, H. (2002). Composition of seed storage proteins changed by glutathione treatment of soybean. Biosci Biotechnol Biochem, 66: 1751-1754.
Basha, S.M.M. and Beevers, L. (1975). The development of proteolytic activity and protein degradation during germination of Pisum sativum (L.). Planta, 124: 77-87.
Beilinson, V., Chen, Z., Shoemaker, R.C., Fischer, R.L., Goldberg, R.B. and Nielsen, N.C. (2002). Genomic organization of glycinin genes in soybean. Theor Appl Gen, 104: 1132-1140.
Bennett, J.O., Krishnan, A.H., Weibold, W.J. and Krishnan, H.B. (2003). Positional effect on protein and oil content and composition of soybeans. J Agric Food Chem, 51: 6882-6886.
Fehr, W.R., Hoeck, J.A., Johnson, S.L., Murphy, P.A., Nott, J.D., Padilla, G.I. and Welke, G.A. (2003). Genotype and environment influence on protein components of soybean. Crop Sci, 43: 511-514.
Granvogl, M., Wieser, H., Koehler, P., Von, T.S. and Schieberle, P. (2008). Influence of sulphur fertilization on the amounts of free amino acids in wheat. Correlation with baking properties as well as with 3-aminoproprionamide and acrylamide generation during baking. J Agric Food Chem, 55: 4271-4277.
Jez J.M. and Krishnan, H.B. (2009). Sulphur assimilation and cysteine biosynthesis in soybean seeds: towards engineering sulphur amino acid content. Modification of seed composition to promote health and nutrition, ASA-CSSA-SSSA Publishing, Madison, pp 249–261.
Kaur, M. (2009). Influence of nitrogen application on various biochemical parameters of different soybean (Glycine max (L.) Merril) genotypes. M.Sc. Thesis, Punjab Agricultural University, Ludhiana.
Kim, W., Jez, J.M. and Krishnan, H.B. (2014). Effects of proteome rebalancing and sulphur nutrition on the accumulation of methionine rich δ-zein in transgenic soybeans. Frontiers Plant Sci, 5: 1-12.
Kopriva, S. and Rennenberg, H. (2004). Control of sulphate assimilation and glutathione synthesis: interaction with N and C metabolism. J Exp Bot 55(404): 1831-1842.
Krishnan, H.B., Bennett, J.O., Kim, W., Krishnan, A.H. and Mawhinney, T.P. (2005). Nitrogen lowers the sulphur amino acid content of soybean (Glycine max [L.] Merr.) by regulating the accumulation of Bowman-Birk protease inhibitor. J Agric Food Chem, 53: 6347-6354.
Laemmli, U.K. (1970). Cleavage of structural protein during the assembly of the heads of bacteriophage T4. Nature, 227: 680-685.
Lowry, O.H., Rosbrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the folin phenol reagent. J Biol Chem, 193: 265-275.
Poysa, V., Woodrow, L. and Yu, K. (2006). Effect of soy protein subunit composition on tofu quality. Food Res Intern, 39: 309-317.
Siddiqui, M.H., Mohammad, F., Khan, M.N. and Khan, M.M.A. (2008). Cumulative effect of soil and foliar application of nitrogen, phosphorus and sulphur on growth, physico-biochemical parameters yield attributes and fatty acid composition in oil of erucic acid-free rapeseed-mustard genotypes. J Plant Nutr, 31: 1284-1298.
Tabe, L., Hagan, N. and Higgins, T.J.V. (2002). Plasticity of seed protein composition in response to nitrogen and sulphur availability. Curr Opin Plant Biol, 5: 212-217.
Taski Hajdukovic, K., Djordjevic, V., Vidic, M., Vujakovic, M., Milosevi, M. and Miladinovic, J. (2008). The main seed storage proteins among high-protein genotypes. Genetika, 40: 9-16.
Tea, I., Genter, T., Naulet, N., Marie, L.M. and Kleiber, D. (2007). Interaction between nitrogen and sulphur by foliar application and its effects on flour bread-making quality. J Agric Food Chem, 87: 2853-2859.
Thanh, V.H. and Shibasaki, K. (1978). Major proteins of soybean seeds. Subunit structure of β-conglycinin. J Agric Food Chem, 26: 692-695.
Yaklich, R.W. (2001). β-conglycinin and glycinin in high protein soybean seeds. J Agric Food Chem, 49: 729-735.
Varier, A. and Dadlani, M. (1992). Effect of ageing on profiles of soluble protein of cotton and esterase isoenzymes of pearl millet seeds. Ind J Plant Physiol, 352: 145-57.
Yi, H., Ravilious, G.E., Galant, A., Krishnan, H.B. and Jez, J.M. (2010). From sulphur to homoglutathione: thiol metabolism in soybean. Amino Acids, 39: 963-978.
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