The effect of three different extraction methods, namely supercritical CO2, soxhlet and solvent methods on the yield, efficiency, physico-chemical properties, nutritional, anti-nutritional composition, antimicrobial and antioxidant activities of moringa (Moringa oleifera Lam.) seed kernel oil was investigated in this study. Oil extraction for SC-CO2 were 37.76 g/100g and 98.43%, observed to be higher than those of soxhlet extraction (29.12 g/100g and 76.29%), and significantly lower than solvent extraction (22.12 g/100g and 57.99%). The physico-chemical composition of the oils showed considerable variation among the extraction methods. The SC-CO2 extracted oil was found to be of superior quality, showing negligible thermal degradation and exhibited significantly (p<0.01) higher nutritional and antioxidant activity and lower anti-nutritional composition than the soxhlet and solvent extracted oils. Oils produced by SC-CO2 and soxhlet extraction methods had antimicrobial activities higher than solvent extracted oil. SCCO2 extracted oil was found to have maximum number of bioactive compounds (14 compounds) followed by solvent (8 compounds) and soxhlet (4 compounds) extracted oil. The results of the study demonstrated that SC-CO2 would be a promising process for the extraction of moringa seed kernel oil of premium quality.
Bioactive compounds, Essential oil, Extraction, Moringa seeds, SC-CO2
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