N. Malini C. R. Anandakumar R. Gnanam S. Hari Ramakrishnan


Callus induction from explants is a critical process in regeneration, micropropagation and transformation of maize (Zea mays L.) plants. Formation of callus from plant tissues on culture is affected by several factors. This study revealed to establish the effect of genotype, source of explants and auxin concentration on callus induction from five genotypes UMI 757 (G1), UMI 615 (G2), UMI 112 (G3), UMI 285 (G4) and CO 1 (G5) and one hybrid CO H (M) 5 (G6). Callus induction of the six maize varieties was investigated using immature embryos (E1), leaf bits (E2), root tips (E3), hypocotyls (E4) and seeds (E5) as explants with different concentrations of hormones. In this study, immature embryo was taken from 10 to 12 days after pollination (DAP) to get maximum response. The highest percentage of callus induction was observed (99.10) in immature embryo culture and seed culture gave the highest percentage of rhizogenic callus formation when compare to immature embryo. Among the genotypes tested, CO H (M) 5 recorded the highest callus induction percentage on (2D2K2) medium composition.




Callus induction, Hormones, Immature embryo, Maize, Seed

Abebe, Z. D., Teffera, W., and Machuka, J. S. (2008). Regeneration of tropical maize lines (Zea mays L.) from mature zygotic embryo through callus initiation. African Journal of Biotechnology Vol. 7 (13): 2181-2186.
Agarwal, P. K., Gosal, S. S., and Sidhu, G. S. (2006). Sequential reduction of 2, 4-D improves whole plant regeneration from long term maintained calli in some indica cultivars of rice. Oryza, 43 (1): 10-15.
Ahmadabadi, M., Ruf, S., and Bock, R.(2007). A leaf-based regeneration and transformation system for maize. Transgenic Res., 16: 437-448.
Al-Abed, D., S. Rudrabhatla, R. Jalla and S. Goldman. 2006. Spilt – seed: a new tool for maize researchers. Planta. 223 : 1355-1360.
Ansari, N. A., (1997). Tissue culture studies in maize (Zea mays L.) Ph.D. (Ag.) Thesis, TNAU, Coimbatore.
Benson, E. E.,(2000). In vitro plant recalcitrance: An introduction. In vitro Cell Dev. Biol. 26: 141-148.
Bijy. K. R., (2002). In vitro screening for drought tolerance in rice (Oryza sativa L.) M.Sc., (Ag.) Thesis, TNAU, Coimbatore.
Binott, J. J., Songa, J. M., Ininda, J., Njagi, E. M. and Machuka, J. (2008). Plant regeneration from immature embryos of Kenyan maize in bread lines and their respective single cross hybrids through somatic emrbyogenic. African Journal of Biotechnology, Vol. 7(8): 981-987.
Bronsema, F. B. B., Van Oostveen, W.J.F. and Van Lammeren, A.A.M. (1997). Comparative analysis of callus formation and regeneration on cultured immature maize embryos of the in bred lines A188 and A 632. Plant Cell, Tissue and Organ Culture., 50: 57-65.
Chand, S. and Sahrawat, A. K. (2000). Efficient plant regeneration from root callus tissues of barley (Hordeum vulgare L.). J. Plant Physiol., 156 : 401-407.
Delporte, F., Mostade, O. and Jacquemin, J.(2001). Plant regeneration through callus initiation from thin mature embryo fragments of wheat. Plant Cell Tissue Organ Cult., 67: 73-80.
Devi, S., PArimala, K., Sravanthi, K. (2016). Gene action and combining ability analysis for yield and its component traits in maize and its component traits in maize (Zea mays L.) Bioscan. (2): 1043 – 1047.
Gomez, K. A. and Gomez, A. A. (1984). Statistical procedures for Agricultural Research. John Wiley and Sons. Inc., New York. pp. 680.
Green, C.E. and Philips, R. C. (1975). Plant regeneration from tissue cultures of maize. Crop Sci., 15: 417-421.
Hodges, T. K., Kamo, K. K., Imbrie, C. W., and Backwar, M. R.(1986). Genotype specificity of somatic embryogenic and regeneration in maize. Biotechnol., 4:219-223.
Huang, X. Q and Wei, Z. M. (2004). High frequency plant regeneration through callus initiation from mature embryos of maize. Plant Cell Rep., 22: 793-800.
Inoue, M and Maeda, E. (1982). Control of organ formation in rice callus using two step culture method. In : Plant Tissue Culture (ed.) Fujiwara, A., Maruxen, Tokyo, pp. 183-184.
Khaleda, L. and Forkan, M.Al. (2006). Genotypic variability in callus induction and plant regeneration through somatic embryogenesis of five deepwater rice (Oryza sativa L.) cultivars of Bangladesh. African J. Biotech., (5): pp-1435-1440.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plantarum., 15: 473-497.
Oduor, R. O., Njagi, E. N. M., Ndung’u, S. and Machuka, J. S. (2006). Invitro regeneration of Dryland Kenyan Maize Genotypes through somatic embryogenesis. International Journal of Botany., 2(2): 146-151.
Omer, R. A., Ali, A. M., Matheka, J. M. and Machuka, J. (2008). Regeneration of Sudanese maize in bred lines and open pollinated varieties. African Journal of Biotechnology, Vol. 7(11): 1759-1764.
Shohael, A. M., Akanda, M. A. L., Parvez, S. and Mahfuja, S.(2003). Somatic embryogenesis and plant regeneration from immature embryo derived callus of inbred maize (Zea mays L.). Biotech., 2 (2): 154-161.
Sikandar, W., Ali, Khan, I. and Munir, I. (2007). Optimization of invitro conditions for Callus induction, proliferation and regeneration in wheat (Triticum aestivum L.) Cultivars. Biotech., 6(3): 420-425.
Tomes, D. T., and Smith, O. S. (1985). The effects of parental genotype on isolation of embryogenic callus from elite maize germplasm. Theor. Appl. Genet., 70:505-509.
Vinothini, S., (2004). Enhancement of variability in drought tolerant varieties of rice (Oryza sativa L.) Through in vitro mutagenesis. M.Sc. (Ag.) Thesis, A. C & R. I., Killikulam, TNAU, Coimbatore.
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Malini, N., Anandakumar, C. R., Gnanam, R., & Ramakrishnan, S. (2018). Effect of harmones on callus induction in Maize (Zea mays L.). Journal of Applied and Natural Science, 10(1), 202-209. Retrieved from https://journals.ansfoundation.org/index.php/jans/article/view/1605
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