Simranjit Singh Upasana Rani U.S. Tiwana Devinder Pal Singh Asmita Sirari


Guar gum (Galactomannan) is extracted from Guar (Cluster bean), which is extensively used in petroleum, food and pharmaceutical industry. Root rot of guaris caused by Fusarium solani EGY1 under Punjab, having sub-tropical climatic conditions. This study was undertaken to evaluate different culture media, grain substrates (sorghum, maize, cowpea, guar and pearl millet), temperatures (20, 25, 30, 35oC), pH levels (5.0, 6.0, 7.0, 8.0), light and darkness for the identification of optimum conditions for the growth and sporulation of the fungus. Czapek’s dox media was found to be best for growth (84.65 mm) and sporulation (1.8 x 104microconidia and 3.0 x 104 macro conidia) of fungus. For mass multiplication of the fungus, sorghum grains proved to be the best substrate. The fungus showed maximum radial growth at temperature of 25oC (84.36 mm) and pH of 6.0 (84.43 mm) whereas sporulation was highest at 30oC (2.0 x 104 microconidia and 3.2 x 104 macroconidia) and pH of 8.0 (1.8 x 104 microconidia and 3.1 x 104 macroconidia) respectively. Continuous light favoured radial growth (84.62 mm) whereas sporulation (1.8 x 104 microconidia and 3.1 x 104 macroconidia) was favoured by darkness.


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Fusarium solani EGY1, Light, Media, pH, Temperature

Adeniyi, D.O., Orisajo, S.B., Fademi, O.A., Adenuga, O.O. and Dongo, L.N. (2011). Physiological studies of fungi complexes associated with cashew diseases. Journal of Agricultural and Biology Science, 6:34-38
Anonymous (2014). An analysis of performance of guar crop in India. Pp. 8-11. CCSNIAM Jaipur. www.ccsniam.gov.in
Ayub, M., Khalid, M., Tariq, M., Nadeem, M.A. and Naeem, M. (2011) Effect of different seedling densities and nitrogen levels on growth, forage yield and quality attributes of cluster (Cyamopsis tetragonoloba Tuab). Journal of Agricuture Technology, 7(5):1409-1417
Bhale, U. N. (2012). Physiological studies of fungicide resistant and sensitive Fusarium oxysporum f.sp. spinaciae. International Journal of Ayurvadic and Herbal Medicine 2:171-75
Chaturvedi, V. C., Gupta, S., Gupta, R. and Shukla, D. N. (2003).Effect of temperature on growth and sporulation of some Fusarium species. Bioved, 14:33-35
Chittem, K. and Kulkarni, S. (2008). Effect of media on the growth of Fusarium oxysporum f.sp. gerberae and Fusarium oxysporum f.sp. dianthi. Karnataka Journal Agricultural Sciences, 21:303-304
Choi, Y.W., Hyde, K.D. and Ho, W.H. (1999). Single spore isolation of fungi. Fungal Diversity, 3:29-38
Diarra, C., Ciotola, M., Hallett, S.G., Hess, D.E. and Watson, A.K. (1996). Mass production of Fusarium oxysporum(M12-4A), a biocontrol agent for Strigahermonthica. Proc 9thIntSymp “Biological Control of weeds”. pp 149-52. Nebraska, America
El-Sayed, A. F., Yasser, M. S., and Nasser (2008) Effect of enviornmental conditions on wilting and root rot fungi pathogenic to solanaceous plants. Plant Pathology Journal 7:27-33
Gangadhara, N. B., Nagaraja, R., Basavaraja, M. K. and Krishna, N. R. (2010). Variability studies of Fusarium oxysporum f.sp. Vanilla isolates. International Journal of Science and Nature,1:12-16
Gupta, V.K., Misra, A.K. and Gaur, R.K. (2010). Growth characteristics of Fusarium spp. causing wilt disease in Psidium guajava L. in India. Journal of Plant Protection Research, 50:452-462
Kausar, P., Chohan, S. and Parveen, R. (2009). Physiological studies on Lasiodiplodia theobromae and Fusarium solani, the cause of Shesam decline. Mycopathology, 7:35-38
Khilare, V.C. and Ahmed, R. (2012).Effect of different media, pH and temperature on the growth of Fusarium oxysporum f.sp. ciceri causing chickpea wilt. International Journal of Advances in Biology, l2:99-102
Kornerup, A. and Wancher, J.H. (1978). Handbook of Colour. 3rd ed. Eyre Methuen Ltd; London, Methuen
Luckew, A., Cianzio, S. and Leandro, L. (2012). Screening method for distinguishing soybean resistance to Fusarium virguliforme in resistant x resistant crosses. Crop Science,52:1-9
Merlin, J.N., Christhudas, I.V.S.N., Kumar, P.P. and Agastian, P. (2013). Optimization of growth and bioactive metabolite production: Fusarium solani. Asian Journal of Pharmaceutical and Clinical Research. 6: 98-103
Pareek, V. and Varma,R. (2015). Fusarium solani a dominant seed borne pathogen in seeds of cluster bean grown in Rajasthan. Bioscience and Biotechnology Research Communication, 8(1): 29-34
Pathak, V.N. (1984) Laboratory Manual of Plant Pathology. Second edition. Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi: 11–12
Singh, S., Rani, U., Sirar,i A. and Tiwana, U. S. (2016). Identification of the pathogen associated with the wilt of guar (Cyamopsis tetragonoloba). Plant Disease Research, 31(1): 6-9
Sortino, O. and Gresta F. (2007) Growth and yield Grain yield response and chilopartillus infestation performance of five guar cultivars in a Mediterranean diverse sorghum crop intercrop management. South environment. Italian Journal of Agronomy, 4:359-364
Velmurugan, P., Lee, Y.H., Venil, C.K., Lakshmanaperumalsamy, P., Chae, J.C. and Oh, B.T. (2009). Effect of light on growth, intracellular and extracellular pigment production by five pigment-producing filamentous fungi in synthetic medium. Journal of Bioscience and Bioengineering, 20:230-231
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Singh, S., Rani, U., Tiwana, U., Singh, D. P., & Sirari, A. (2017). Investigation of optimum conditions for the growth of Fusarium solani EGY1 causing root rot of guar (Cyamopsis tetragonoloba L.). Journal of Applied and Natural Science, 9(4), 2249–2254. https://doi.org/10.31018/jans.v9i4.1519
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