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Harshavardhan D. Joshi V. K. Tiwari Rupam Sharma Subodh Gupta W. S. Lakra Upasana Sahoo

Abstract

The aim of present work was to develop a new Fadrozole (FDZ)-loaded Poly (D,L-lactide-co– glycolide) lactide:glycolide (50:50)(PLGA) nanoparticles for effective delivery of the masculinization drug, Fadrozole, as an alternative to commercially available masculinization agents like testosterone (dietary supplementation of 17 ?- methyltestosterone) which are steroids and banned in most EU countries. The FDZ-loaded PLGA NPs were pre-pared by solvent displacement technique. The particle size of FDZ-loaded PLGA NPs was analyzed using LICOMP particle size analyser. It was found to be in the range of 60±66.7 nm to 560±66.7 nm with average size of 201.4±66.7 nm, where the Zeta potential was estimated to be about -20.82 mV, a series of experiments were carried out to induce masculinization using FDZ-loaded PLGA nanoparticles during the sex differentiation period. Tilapia, Oreochromis niloticus fry were treated with FTZ-loaded PLGA nanoparticles at dosages 5, 25, 50 and 100ppm/kg diet for 10, 15 and 30 days. The results indicated an increase in the proportion of males with dosage and duration of treatment. The male percentage was 92.35±0.86 for T7(50 ppm) at 10 days, 97.76±1.12 for T7 (100 ppm) at 15 days and 100 % for both T6 (50ppm) and T7 (100 ppm) at 30 days. This is first time done by using nanotechnology efficiently in Tilapia species which is very important Fresh water aquaculture species in present era. Which showed increase the male population with lesser dose of nano-encapsulated Fadrozole (FDZ) loaded PLGA nanoparticles drug as compared with naked control Fadrozole (FDZ) drug delivery.

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Keywords

Bio-dégradable, Fadrozole, Masculinisation, Nanoparticle, PLGA, Oreochromis niloticus

References
Brannon-Peppas, L. (1995). Recent advances on the use of biodegradable micro particles and nanoparticles in controlled drug delivery. Int. J. Pharm. 116: 1–9.
Brigger, I., Dubernet, C., Couvreur, P. (2002). Nanoparticles in cancer therapy and diagnosis. Adv. Drug Deliv. Rev. 54: 631–651.
Chiang E.F.L., Yan Y.L., Guiguen Y., Postlethwait J. and Chung B. (2001). Two Cyp19 (P450 Aromatase) Genes on Duplicated Zebrafish Chromosomes Are Expressed in Ovary or Brain. Mol. Biol. Evol. 18 (4): 542-550.
Das,T., Chattopadhyay,R., Ghosh,S., Goswami, S., Chattopadhyay D., Chakravarty, B. and Kabir,S. N. (2007). Role of an estrogen-upregulated 64.0-kDa uterine fluid glycoprotein in improving fertility in women. American Society for Reproductive Medicine, Published by Elsevier Inc. 87 (2): 343-350.
Hughes, G.A., (2005). Nanostructure mediated drug delivery. Nanomedicine 1: 22–30.
Komatsu, T., Nakamura, S., Nakamura, M. (2006). Masculinization of female golden rabbitfish Siganus guttatus using an aromatase inhibitor treatment during sex differentiation. Comp. Biochem. Physiol. C. Toxicol. Pharmacol. 143: 402–409
Khawa H. L., Ponzonia R.W., Dantingb M. J. C. (2008). Estimation of genetic change in the GIFT strain of Nile tilapia (Oreochromis niloticus) by comparing contemporary progeny produced by males born in 1991 or in 2003.Aquaculture 275, 64-69.
Kwon, J.Y., Hoghponah, H., Kagson-Hurtodo, L.M., MC Andrew, B.J. and Penman, D.J. (2000). Masculinization of genetic female Nile tilapia (O.nioticlus) by dietary administration of an aromatase inhibitor during sexual differentiation. J.Exp.Zool., 287:46-53
Kwon, J.Y., MC Andrew, B.J. and Penman, D.J. (2001). Cloning of brain aromatase gene and expression of brain and ovarian aromatase gene during sexual differentiation in genetic male and female Nile tilapia (O.niloticus). Mol.Reprod. Dev., 59:359-370.
Kwon, J.Y., MC Andrew, B.J. and Penman, D.J. (2002). Treatment with an aromatase inhibitors suppresses high temperature feminization of genetic male (YY) Nile tilapia. J.Fish.Biol. 60:625-636.
Mondal, N., PAL, T. K.,Ghosal S. K. (2008). Development, physical characterization, micromeritics and in vitro release kinetics of letrozole loaded biodegradable nanoparticles. Pharmazie 63: 361–365.
Mondal, N., Halder, K. K., Kamila, M. M., Debnath, M. C., Pala, T. K., Ghosal, S.K., Sarkar, B. R. and Ganguly, S. (2010). Preparation, characterization, and biodistribution of letrozole loaded PLGA nanoparticles in Ehrlich Ascites tumor bearing mice. International Journal of Pharmaceutics. 397: 194–200.
Otilia M. K., Rubinstein I., Onyuksel H. (2005).Role of nanotechnology in targeted drug delivery and imaging: a concise review. Nanomedicine 1: 193-212.
Park, J. S., Yang, H. N., Jeon, S. Y., Woo, D. J., Kim, M. S. and Park, K. H. (2012). The use of anti-COX2 siRNA coated onto PLGA nanoparticles loading dexamethasone in the treatment of rheumatoid arthritis. Biomaterials. 33: 8600-8612.
Pandian, T.J. and Varadaraj, K. (1987). Techniques to regulate sex ratio and breeding in tilapia. Curr. Sci., 56: 337– 343.
Piferrer, F. (2001). Endocrine sex control strategies for the feminization of teleost fish. Aquaculture., 197: 229– 281.
Tchoudakova A. and Callard G. V. (2011). Identification of Multiple CYP19 Genes Encoding Different Cytochrome P450 Aromatase Isozymes in Brain and Ovary. Endocrinology. 139: 2179-2189.
NABARD (2016). The National Bank for Agricultural and Rural Development (NABARD) retrieved from https://www.nabard.org/search-result.aspx?S=aquaculture
Tzchori, I., Zak, T., and Saches, O. (2004). Masculinization of genetic females of the common carp (Cyprinus carpio) by dietary administration of an aromatase inhibitor. The.Israel. J.Aqua, 56(4): 239-246.
Vauthier, C., Dubernet, C., Chauvierre, C., Brigger, I., Couvreur, P. (2003). Drug delivery to resistant tumors: the potential of poly (alkyl cyanoacrylate) nanoparticles. J. Control. Release 93:151–160.
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Joshi, H. D., Tiwari, V. K., Sharma, R., Gupta, S., Lakra, W. S., & Sahoo, U. (2017). Nanotechnological innovation for the production of daughter less Tilapia, Oreochromis niloticus (Linnaeus, 1758). Journal of Applied and Natural Science, 9(4), 1921–1925. https://doi.org/10.31018/jans.v9i4.1464
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